Mechanisms of endometrial tissue reconstitution and basic studies on establishment of endometrial tissue regeneration therapy

子宫内膜组织重建机制及子宫内膜组织再生疗法建立的基础研究

基本信息

批准号：
15591773
负责人：
UMESAKI Naohiko
金额：
$ 2.24万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591773/
关键词：
endometrium stem cell endometriosis adenomyosis growth factor cell differentiation 子宮内膜間質細胞

项目摘要

(1)Characterization of human endometrial stem-like cells : In the present study, cellular heterogeneity and functional subpopulations of human endometrial cells were identified. Results of the study are in preparation for submission.(2)In vitro cell proliferation and differentiation of human endometrial stem-like cells : We have discovered at least 2 types of novel differentiation pathways of human endometrial cells. And we examined in vitro differentiation of the sorted functional cell populations from endometrial cells in order to investigate make clear cellular relationships among the populations. The results are prepared for submission.(3)Re-constitution of murine endometrial tissue in vivo using green mouse-derived endometrial stem-like cells : We have been investigating in vivo re-constitution of murine endometrium in wild mouse with the sorted green mouse cells but have not completed it yet. These experiments will be continued even though the project period is over.(4)Endometrial re-generation in vivo in murine uterus with human endometrial stem-like cells : We have already proven that re-constitution of human endometrial tissue in vivo in mouse needs several cellular subpopulations but not one cell population. Moreover, we have investigated various bioactive substances that regulate re-constitution in vitro and re-generation of human endometrium in murine uteri in vivo. The results are prepared for submission.(5)Identification of specific antigens on human endometrial stem-like cells : We have established more than 5000 of hybridomas that produce anti-endometrial stem-like cells in order to sort the stem-like cells from human endometrial tissue and try to re-constitute human endometrial tissue in SCID or nude mouse uterus. However, we have not found any useful antibody to apply for further experiments yet.

(1)人子宫内膜干细胞样细胞的表征：在本研究中，鉴定了人子宫内膜细胞的细胞异质性和功能亚群。研究结果正在准备提交中。(2)人子宫内膜干细胞的体外细胞增殖和分化：我们发现了至少2种新的人子宫内膜细胞分化途径。我们检查了子宫内膜细胞中分选的功能细胞群的体外分化，以研究清楚细胞群之间的细胞关系。 (3)使用绿色小鼠来源的子宫内膜干细胞体内重建小鼠子宫内膜组织：我们一直在研究用分选的绿色小鼠在野生小鼠体内重建小鼠子宫内膜组织细胞，但尚未完成。即使项目期结束，这些实验仍将继续进行。(4)用人子宫内膜干细胞在小鼠子宫体内进行子宫内膜再生：我们已经证明，在小鼠体内重建人类子宫内膜组织需要多种细胞亚群，但不是一种细胞群。此外，我们还研究了调节体外重构和体内小鼠子宫内膜再生的各种生物活性物质。 (5)人子宫内膜干细胞特异性抗原的鉴定：我们已经建立了5000多个产生抗子宫内膜干细胞的杂交瘤，以便从人子宫内膜干细胞中分选干细胞。子宫内膜组织并尝试在SCID或裸鼠子宫中重建人类子宫内膜组织。然而，我们尚未找到任何有用的抗体可用于进一步的实验。