Mechanisms of postmenstrual endometrial remodeling and intercellular signalings during embryo implantation

胚胎植入过程中经后子宫内膜重塑和细胞间信号传导的机制

基本信息

批准号：
17591751
负责人：
UMESAKI Naohiko
金额：
$ 2.24万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591751/
关键词：
endometrium embryo implantation cytokine endometriosis regeneration medicine

项目摘要

For mouse hepatitis virus pollution of university animal center in spring of 2006, all our mice were killed and the animal experiments were ceased. Since virus infection was re-appeared half a year after disinfection, however, the animal experiment center has been closed yet. We, therefore, had to switch plans of this study.(1) Analysis of mouse endometrial remodeling process and identification of murine endometrial stem cells:Short-term endometrial rebuilding was succeeded in by mixed culture transplant of plural endometrial cell strains, but animal experiment is stopped by the above reason, and long-term observation cannot be finished.(2) Analysis of human endometrial remodeling process and identification of human endometrial stem cells:<1> Cellular characterization of human endometrial stromal cells(i) Influence of various bioactive substances: Influence such as various cytokines, bioactive substances, cell death inducers such as anticancer drugs, siRNA experiments were examined (pr … More eparation in submission). Involvement of TRAIL and TRAIL receptor was made clear by DAPK knockdown experiments (submitted).(ii) Apoptosis sensitivity: Endometrial stem-like cells had resistance to various apoptotic signals such as anticancer drugs and Fas stimulants.(iii) Differentiation activity: Partial differentiation was confirmed by mixed culture experiments.<2> Establishment of in vitro differentiation of human endometrial stem-like cells to make differentiation pedigree of human endometrial cellsPartial differentiation phenomenon was confirmed by mixed culture experiments, but the experiments are ongoing now. Establishment of endometrial reproduction medical treatment by mouse intrauterine transplantation of two human endometrial stem-like cells. Animal experiments have been stopped because of the above reasons. Three human endometrial stem-like cell-specific antigen were identified. In order to establish hybridomas producing anti-endometrial stem cell monoclonal antibodies balb/c mice were immunized with human endometrial stem-like cells. However, immunized mice were killed due to contamination of the animal center. When the animal experiments were allowed, the plan will be re-started. Less

2006年春季，学校动物中心出现小鼠肝炎病毒污染，我们将小鼠全部杀死，动物实验停止，但消毒半年后又出现病毒感染，动物实验中心至今仍处于关闭状态。因此，我们不得不改变本研究的计划。(1)小鼠子宫内膜重塑过程分析及小鼠子宫内膜干细胞鉴定：通过多种子宫内膜细胞混合培养移植，成功实现短期子宫内膜重建。（2）人子宫内膜重塑过程分析及人子宫内膜干细胞鉴定：<1>人子宫内膜基质细胞的细胞表征（i））各种生物活性物质的影响：检查了各种细胞因子、生物活性物质、抗癌药物等细胞死亡诱导剂、siRNA实验等的影响（TRAIL和TRAIL的参与）。通过DAPK敲低实验明确了TRAIL受体（已提交）。（ii）凋亡敏感性：子宫内膜干细胞样细胞对抗癌药物和Fas刺激剂等多种凋亡信号具有抵抗力。（iii）分化活性：通过混合法证实部分分化。 <2>建立人子宫内膜干细胞体外分化的体系，制作人子宫内膜细胞的分化谱系，通过混合培养实验证实了部分分化现象，但目前正在进行的通过小鼠子宫内移植两种人类子宫内膜干细胞进行子宫内膜生殖治疗的实验由于上述原因已被停止。为了建立产生抗子宫内膜干细胞单克隆抗体的杂交瘤，用人子宫内膜干细胞样细胞对balb/c小鼠进行免疫，但免疫小鼠因动物污染而被处死。当动物实验被允许时，该计划将重新启动。