Therapeutic effect of hematopoietic stem cell transplantation in combination with tissue regenerative factor on systemic sclerosis

造血干细胞移植联合组织再生因子治疗系统性硬化症的疗效观察

• 批准号: 15591071
• 负责人: IWASAKI Tsuyoshi
• 金额: $ 2.11万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591071/
• 关键词: systemic sclerosis; HGF; hematopoietc stem cell transplantation; gene therapy; HVJ-liposome; 骨髄移植; HVJリポゾーム

1)Transplantation of T-cell depleted bone marrow cells from BALB/c mice improved skin thickness by Tsk mice2)HGF gene transfection improved skin thickness by Tsk mice.3)Combination of bone marrow transplantation and HGF gene therapy significantly improved skin thickness by Tsk mice but did not improved pulmonary fibrosis.4)Transplantation of T-cell depleted bone marrow cells shifted immune responses from Th2 to Th1.5)Donor cells were engrafted at 2 weeks after transplantation, but were rejected at 4 weeks after transplantation.

1)移植来自BALB/c小鼠的T细胞耗尽的骨髓细胞改善了Tsk小鼠的皮肤厚度2)HGF基因转染改善了Tsk小鼠的皮肤厚度。3)骨髓移植和HGF基因治疗相结合显着改善了Tsk小鼠的皮肤厚度小鼠，但没有改善肺纤维化。4) 移植 T 细胞耗尽的骨髓细胞将免疫反应从 Th2 转移到 Th1。5) 供体细胞被植入移植后2周，但移植后4周被排斥。