Effect of proton pump inhibitor and COX2 inbitor upon Barrett's intestinal metaplasia.

质子泵抑制剂和COX2抑制剂对Barrett肠化生的影响。

• 批准号: 15590664
• 负责人: ENDO Takao
• 金额: $ 2.24万
• 依托单位: First department of internal medicine, Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590664/
• 关键词: Barrett's esophagus; PPI; chemo prevention; proton pump inhibitor(PPI); Barrett粘膜; 細胞増殖能; 酸分泌抑制剤

We examined whether proton pump inhibitor(PPI) may diminish the risk of carcinogenesis. in Barrett's esophagus (BE). In our study, endoscopic biopsy specimens of BE was analyzed for change in the expression of Ki-67, COX-2, CDX-2, sulfo-Lewisa by immunohistochemistry before and after treatment with PPI.6 months after treatment with PPI, there was a significant decrease in the expression of Ki-76 (cell proliferation maker) and COX-2 (proliferation-related agent). And there was a tendency to decrease in the expression of CDX-2(intestinal transcription factor) and sulfo-Lewisa (intestinal mucin).In contrast, treatment with histamin2 receptor antagonist (H2RA) showed no significant decrease in the expression of Ki-67, COX-2, CDX-2, sulfo-Le^a.We evaluated that treatment with PPI would reduce the expression of COX-2 and consequently cell proliferation would decrease.It was reported that the expression of between COX-2 and CDX-2 would be associated with p38 MAPK pathway. Accordingly, we examined the expression of Phospho-p38 in BE before and after treatment with PPI.There was a significant decrease in the expression of -Phospho-p38 in both epithelial cells and stromal cells in BE before and-after treatment with- PPL.Therefore, we concluded- that PPI- would inhibit- the- expression of both COX-2 and CDX-2 via p38. MAPK pathway and that consequently cell proliferation and intestinal metaplasia. would. diminish.

我们研究了质子泵抑制剂（PPI）是否可以降低致癌风险。位于巴雷特食管 (BE)。本研究采用免疫组织化学方法分析BE内镜活检标本在PPI治疗前后Ki-67、COX-2、CDX-2、sulfo-Lewisa表达的变化。PPI治疗后6个月， Ki-76（细胞增殖标志物）和 COX-2（增殖相关剂）的表达显着下降。 CDX-2（肠转录因子）和sulfo-Lewisa（肠粘蛋白）的表达有下降趋势。相比之下，组胺2受体拮抗剂（H2RA）治疗后Ki-67的表达没有明显下降。 , COX-2, CDX-2, sulfo-Le^a。我们评估用PPI处理会降低COX-2的表达，从而减少细胞增殖。据报道，之间的表达COX-2 和 CDX-2 与 p38 MAPK 通路相关。因此，我们检测了用PPI治疗前后BE中Phospho-p38的表达。用-PPL治疗前后，BE中上皮细胞和基质细胞中-Phospho-p38的表达显着降低。因此，我们得出结论，PPI 将通过 p38 抑制 COX-2 和 CDX-2 的表达。 MAPK 通路进而导致细胞增殖和肠化生。会。减少。

项目成果

Hamamoto Y, Endo T: "Usefulness of narrow-band imaging endoscopy for diagnosis of Barrett's esophagus."J Gastroenterol. 39. 14-20 (2004)

Hamamoto Y，Endo T：“窄带成像内窥镜检查在巴雷特食管诊断中的有用性。”J Gastroenterol。

Satoh A, Toyota M, Endo T et al.: "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."Cancer Res. 63. 8606-8613 (2003)

Satoh A、Toyota M、Endo T 等人：“胃癌中 CHFR 的表观遗传失活和对微管抑制剂的敏感性。”Cancer Res。

Appearance of enhanced tissue features in narrow-band endoscopic imaging

• DOI: 10.1117/1.1695563
• 发表时间: 2004-05-01
• 期刊: JOURNAL OF BIOMEDICAL OPTICS
• 影响因子: 3.5
• 作者: Gono, K;Obi, T;Endo, T
• 通讯作者: Endo, T

Interplay of insulin-like growth factor-II, insulin-like growth factor-I, insulin-like growth factor-I receptor, COX-2, and matrix metalloproteinase-7, play key roles in the early stage of colorectal carcinogenesis

• DOI: 10.1158/1078-0432.ccr-04-0875
• 发表时间: 2004-12-01
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Nosho, K;Yamamoto, H;Imai, K
• 通讯作者: Imai, K

Endoscopic observation of tissue by narrowband illumination

• DOI: 10.1007/s10043-003-0211-8
• 发表时间: 2003-07-01
• 期刊: OPTICAL REVIEW
• 影响因子: 1.2
• 作者: Gono, K;Yamazaki, K;Endo, T
• 通讯作者: Endo, T