Two intercellular lamellar structures in stratum corneum and their functional regulation mechanism

角质层两种细胞间层状结构及其功能调节机制

基本信息

批准号：
15540397
负责人：
HATTA Ichiro
金额：
$ 2.24万
依托单位：
Fukui University of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

The outermost layer of skin, stratum corneum (SC), is composed of corneocytes and intercellular lipid matrix. The matrix works as not only the main barrier but also the pathway of water, drug, etc. In mammalian SCs, for the longitudinal arrangement of the lipid molecules, long and short lamellar structures with a repeat distance of about 13 nm and about 6 nm, respectively, have been observed by small-angle X-ray diffraction. With increasing water content in SC, the diffraction peak position for the diffraction of long lamellar structure (LLS) is almost unchanged. On the other hand, the position of the diffraction of short lamellar structure (SLS) markedly shifts towards lower angle, suggesting that SLS exhibits swelling with the increase of the water content. Furthermore, we found that the widths of the diffraction peaks of both LLS and SLS become narrow simultaneously at a water content of 20-30 wt%. The present results indicate that LLS and SLS interact with each other, swelling of SLS takes place, and as a result at the water content of 20-30 wt% the both LLS and SLS are stabilized simultaneously. Besides, for the lateral arrangement of the lipid molecules, hexagonal and orthorhombic hydrocarbon chain packings have been observed by wide-angle X-ray diffraction. From the temperature dependence of the simultaneous small- and wide-angle X-ray diffraction, we revealed that the intercellular lipid matrix forms two domains which, at room temperature, are composed of the long lamellar structure with the hexagonal hydrocarbon-chain packing and the short lamellar structure with the orthorhombic hydrocarbon-chain packing.

皮肤最外层的角质层（SC）由角膜细胞和细胞间脂质基质组成。对于哺乳动物SCS中的水，药物等的途径，基质的作用不仅是脂质分子的纵向排列，在哺乳动物SC中的途径，长和短的层状结构分别重复约为13 nm和约6 nm，而小型角X射线X射线扩散已被观察到。随着SC中水分含量的增加，长层状结构（LLS）衍射的衍射峰位置几乎没有变化。另一方面，短层状结构（SLS）的衍射位置明显转移到较低角度，这表明SLS随水含量的增加而表现出肿胀。此外，我们发现，LLS和SLS的衍射峰的宽度同时在20-30 wt％的水含量下同时变窄。目前的结果表明LLS和SLS相互相互作用，SLS的肿胀发生，因此，在20-30 wt％的水含量下，LLS和SLS同时稳定。此外，对于脂质分子的横向排列，通过广角X射线衍射可以观察到六边形和矫正碳氢化合物链填料。从同时的小角度和广角X射线衍射的温度依赖性来看，我们揭示了细胞间脂质基质形成两个结构域，在室温下，这些结构域由长层状结构组成，由长层状结构组成，具有六边形的碳氢化合物包装，并带有短层填料和短层结构，并带有带有矫正水合物的扁平式水容的固定包装。