Molecular recognition mechanism on biomembrane surface in primary biological functioning

初级生物功能中生物膜表面的分子识别机制

• 批准号: 07408018
• 负责人: HATTA Ichiro
• 金额: $ 17.73万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07408018/
• 关键词: biomembrane; phospholipid; phase transition; X-ray diffraction; calorimetry; interaction; molecular recongnition; interdigitated structure; 分子認識; インターディジテイテッド構造

Microscopic structure and thermodynamic approach is quite important to investigate biological functioning on biomembrane surface. From this point of view, we developed a method to perform simultaneous X-ray diffraction and differential scanning calorimetry measurements. We applied this method to studies on aqueous dispersions of phospholipid and phospholipid/phosphatidylglycerol. In the former, we obtained the results that the X-ray diffraction intensity is proportional to the transition enthalpy. In the latter, we found the existence of H_<II> structure that plays an important role in biological functioning. As examples for interaction between a phospholipid molecule and a small molecule, we studied systems of phospholipid/alcohol and phospholipid/sugar. In the former, we analyzed the interdigitated structure and proposed a micro-structural model. In the latter, depending on sugars the condition that stabilizes liquid-crystalline phase was revealed. Furthermore, as an example of interaction between a phospholipid molecule and a small molecule, we studied a system of phospholipid/water by thermal analysis and X-ray diffraction. From this study, we found the number of water molecule incorporated in the interbilayer. Furthermore, we studied the structure of a monolayer of fatty acids formed on a graphite surface by scanning tunneling microscopy with atomic resolution and this method offers possibility to carry out a study on interaction at atomic scale.

微观结构和热力学方法对于研究生物膜表面的生物功能非常重要。从这个角度来看，我们开发了一种同时进行 X 射线衍射和差示扫描量热测量的方法。我们将该方法应用于磷脂和磷脂/磷脂酰甘油的水分散体的研究。在前者中，我们得到了X射线衍射强度与跃迁焓成正比的结果。在后者中，我们发现H_<II>结构的存在在生物功能中发挥着重要作用。作为磷脂分子和小分子之间相互作用的例子，我们研究了磷脂/醇和磷脂/糖的系统。在前者中，我们分析了叉指结构并提出了微观结构模型。在后者中，根据糖，揭示了稳定液晶相的条件。此外，作为磷脂分子和小分子之间相互作用的例子，我们通过热分析和 X 射线衍射研究了磷脂/水系统。从这项研究中，我们发现了双层间结合的水分子的数量。此外，我们通过原子分辨率的扫描隧道显微镜研究了石墨表面形成的单层脂肪酸的结构，该方法为在原子尺度上进行相互作用研究提供了可能性。

项目成果

I.Hatta: "High Precision Heat Capacity Measurement by Dynamic Differential Scanning Calorimetry" Jpn.J.Appl.Phys.35・7A. L858-L860 (1996)

I.Hatta：“通过动态差示扫描量热法进行高精度热容量测量”Jpn.J.Appl.Phys.35・7A（1996）。

H.Takahashi: "Structural and Thermotropic Properties of Calcium-Dimyristoylphosphatidic Acid Complexes at Acidic and Neutral pH Conditions" Biophysical Journal. 69. 1464-1472 (1995)

H.Takahashi：“酸性和中性 pH 条件下钙-二肉豆蔻酰磷脂酸复合物的结构和热致性质”生物物理学杂志。

H.Takahashi: "On exothermic transformation from metastable gel phase to stable crystalline phase of fully hydrated dimyristoylphosphatidylethanolamine in heating scan" Chem.Phys.Lipids. 89. 83-89 (1997)

H.Takahashi：“关于在加热扫描中完全水合的二肉豆蔻酰磷脂酰乙醇胺从亚稳态凝胶相到稳定结晶相的放热转变”Chem.Phys.Lipids。

I.Hatta: "Simultapeous differential scanning calorimetry and time-resolved x-ray diffraction of lipid-water system. I.Relationship between chain melting and endothermic heat at the main transition of a dipalmitoylphosphatidylcholine-water system" Chem.Phy

I.Hatta：“脂质-水系统的同步差示扫描量热法和时间分辨 X 射线衍射。I.二棕榈酰磷脂酰胆碱-水系统主转变时链熔化与吸热之间的关系” Chem.Phy

H.Takahashi: "Cubic Phases in Hybrated 1:1 and 1:2 Dipalymitoyl-phosphatidylcholine-Dipalmitoylglycerol Mixtures" Biophys.J.70・3. 1407-1411 (1996)

H. Takahashi：“混合 1:1 和 1:2 二棕榈酰-磷脂酰胆碱-二棕榈酰甘油混合物中的立方相”Biophys.J.70・3（1996）。