Molecular Epidemiology of Thyroid Diseases around Semiparatinsk, Kazakhstan and Altai region, Russia

哈萨克斯坦塞米帕拉金斯克和俄罗斯阿尔泰地区甲状腺疾病的分子流行病学

• 批准号: 12576020
• 负责人: YAMASHITA Shunichi
• 金额: $ 6.34万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576020/
• 关键词: Thyroid Cancer; Molecular Epidemiology; Chernobyl; Semipalatinsk; Genetic Diagnosis; Mitochondrial DNA; Cloning; Oncogene; 放射性発癌; 甲状腺がん; ret; β-catenin

【○!1】 Our first molecular biology study on thyroid carcinogenesis using the samples from Semipalatinsk indicated the necessity of research on clarification of radiation-associated signature genes in human thyroid cancers (Lancet 354 : 1528, 1999). Our preliminary data on urinary iodine measurement has been reported (Endocrine J 50 : 85, 2003), indicating the proper supplementation of iodine around Semipalatinsk.【○!2】 Our joint project on Chernobyl Thyroid Tissue Bank has been smoothly running (Science 289 : 2283, 2000). According to this idea, the establishment of Tissue Bank or Blood Bank has been now tried under the negotiation with domestic and international cooperation.【○!3】 The psychosocial problems in the local residents around radio-contaminated areas have been analyzed (Lancet 356 : 257, 2000). Separately the radiosensitivity of younger children compared to adults has been proved and the dramatic increase of childhood thyroid cancer is mostly likely to the short-lived radianucletids (radiation fallout) from Chernobyl (Lancet 358 : 1965, 2001).【○!4】 Genetic analysis of short statures in the former USSR has indicated the specific Prop 1 gene mutation pattern and given a clue of the epitology of combined pituitary hormone deficiency syndrome (Am J Roentogenol 174 : 555, 2000).【○!5】 Low-dose radiation inducible genes have been clarified using cultured human thyroid cells, among of which is serum-inducible kinase (BBRC 289 : 491, 2001). We have also clarified the characteristics of intracellular signal transduction in human thyroid cells (Oncogene 20 : 989, 2001, J Clin Endocrinol Metab 87 : 3433, 2002, Thyroid 12 : 95, 2002, Thyroid 12 : 435, 2002). Furthermore the large deletion of mitochondrial DNA is proved to be a kind of marker of radiation-associated thyroid carcinogenesis (Cancer Res 62 : 7031-7041, 2002)

【○！1】 我们使用塞米巴拉金斯克样本进行的第一项甲状腺癌发生的分子生物学研究表明有必要研究澄清人类甲状腺癌中与辐射相关的特征基因（Lancet 354：1528，1999）。据报道（Endocrine J 50：85，2003），表明适当补充碘塞米巴拉金斯克。【○！2】 我们的切尔诺贝利甲状腺组织库联合项目进展顺利（Science 289 : 2283, 2000） 根据这个想法，目前正在与国内协商建立组织库或血库。 【○!3】 分析了辐射污染地区周边居民的社会问题（Lancet 356 : 257, [○!4】前苏联身材矮小的遗传分析揭示了特定的Prop 1基因突变模式并给出了线索联合垂体激素缺乏综合征的表观学 (Am J Roentogenol 174 : 555, 2000)。【○!5】 使用培养的人甲状腺细胞阐明了低剂量辐射诱导基因，其中包括血清诱导激酶 (BBRC 289 : 491, 2001）我们还阐明了人甲状腺细胞内信号转导的特征（Oncogene 20）。 : 989, 2001, J Clin Endocrinol Metab 87 : 3433, 2002, Thyroid 12 : 95, 2002, Thyroid 12 : 435, 2002）此外，线粒体DNA的大量缺失被证明是辐射相关甲状腺的一种标志物。致癌作用（癌症研究 62：7031-7041， 2002）

项目成果

M.Hoshi et al.: "Radiocesium in children resideing in the western distinct of the Bryansk"Health Phys. 79. 182-186 (2000)

M.Hoshi 等人：“居住在布良斯克西部地区的儿童中的放射铯”健康物理学。

S.Yamashita, et at.: "Molecular epidemiology of childhood thyroid cancer around Chernobyl"Radiation and Homeostasis. 556 (2001)

S.Yamashita 等人：“切尔诺贝利周围儿童甲状腺癌的分子流行病学”辐射和稳态。

N.Mitsutake, et al.: "PKC delta mediates ionizing radiation-induced activation of c-Jun NH2-terminal kinase through MKK7 in human thyroid cells"Oncogene. 20巻・8号. 989-996 (2001)

N. Mitsutake 等人：“PKC delta 通过人甲状腺细胞中的 MKK7 介导电离辐射诱导的 c-Jun NH2 末端激酶激活”Oncogene 第 20 卷，第 8 期。989-996 (2001)

N.Mitsutake, et al.: "PKC _mediated ionizing radiation-induced activation of c-Jun NH2-terminal kinase through MKK7 in human thyroid cells"Oncogene. 20巻・8号. 989-996 (2001)

N. Mitsutake 等人：“人甲状腺细胞中通过 MKK7 PKC 介导的电离辐射诱导的 c-Jun NH2 末端激酶激活”Oncogene 第 20 卷，第 8 期。989-996 (2001)

Y.Sautin, et al.: "Ceramide-induced apoptosis of human thyroid cancer cells resistant to apoptosis"Thyroid. 10. 733-740 (2000)

Y.Sautin 等人：“神经酰胺诱导的抗细胞凋亡的人甲状腺癌细胞凋亡”甲状腺。