Production of hypoxic adaptation model in rat

大鼠缺氧适应模型的制作

基本信息

批准号：
12558002
负责人：
OHKUWA Tetsuo
金额：
$ 7.36万
依托单位：
Nagoya Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

All experiments (hybrid, pregnancy, nurse and exercise) were carried out in the sterile, hypoxia chamber. At the age of 4 weeks, the Progenitor's group (PI) Wistar male and female rats were randomly assigned to the following groups : male rats : hypoxia and exercise (MHE), hypoxia and sedentary (MHS). The male and female exercising groups were allowed free access to the running wheel. The numbers of revolution of the wheel (1.16m, circumference) were recorded by using a mechanical counter that was read daily. Male and female rats who did the highest total amount of running distance during 8 weeks were selected. The selected male and female rats were hybridized. The children (the first of high-altitude native rats ; F1) were separated from their mother after postnatal day 21. The male rats of F1 were randomly divided into three groups : MHE-F1, MHS-F1 and normoxic and sedentary low-altitude native rats, as control group. At the age of 18 weeks, the P1, F1, F3 (the third high-altitude na … More tive rats), F5(the fifth) and F7(the seventh) were anesthetized by ether and sacrificed by sampling of blood from the aorta, and brain, heart, liver, diaphragm, skeletal muscle (soleus and gastrocnemius white muscle). The whole brain was divided into the following four regions : cerebellum, frontal lobe, striatum, hippocampus.Study (1) : Monoamines levels of the hypoxic exercise group in high-altitude native significantly decreased in the frontal lobe, cerebellum and striatum, compare with the other groups. These study suggest that hypoxic exercise decreased monoamine levels in whole brain, and that sensitivity to hypoxia and/or exercise differed according to brain regions.Study (2) No significant differences were found in the norepinephrine levels for the diaphragm and soleus muscle among the three groups. This study clarified that hypoxia elicits a decreased sympathetic response in the lover, However, it cause an increase in the response in the heart and gastrocnemius white muscle. These results suggest that the sympathetic response to long -term hypoxia changes with the rat tissues.Study (3) The present study confirmed that moderate hypoxia and hypoxic training induce attenuate the liver DNA damage and decrease the liver GSH level and GR activity. These results indicate that moderate hypoxia and hypoxic training result in deceased oxidative stress. Less

所有实验（杂交、妊娠、哺乳和运动）均在无菌、缺氧室中进行。4周龄时，祖细胞组（PI）Wistar雄性和雌性大鼠被随机分配至以下组：雄性大鼠：缺氧和运动（MHE）、缺氧和久坐（MHS）允许男性和女性运动组自由进入跑轮（1.16m，周长）。通过使用每天读取的机械计数器记录，选择8周内跑动总距离最高的雄性和雌性大鼠进行杂交。；F1)在出生后21天后与母亲分离。F1的雄性大鼠随机分为三组：MHE-F1、MHS-F1和常氧、久坐的低海拔本地大鼠，作为对照组。 18周后，将P1、F1、F3（第3只高原实验鼠）、F5（第5只）、F7（第7只）乙醚麻醉后处死，取主动脉、脑部血液，心脏、肝脏、膈肌、骨骼肌（比目鱼肌和腓肠肌白肌）整个大脑分为以下四个区域：小脑、额叶。研究(1)：与其他组相比，低氧运动组的额叶、小脑和纹状体的单胺水平显着降低。这些研究表明，低氧运动降低了高原人的单胺水平。研究（2）去甲肾上腺素水平没有发现显着差异这项研究阐明，缺氧会导致爱人的交感神经反应减弱，但会导致心脏和腓肠肌白肌的反应增加。这些结果表明，交感神经反应对长 -研究(3)本研究证实，适度缺氧和低氧训练可减轻肝脏DNA损伤，降低肝脏GSH水平和GR活性。中度缺氧和低氧训练会导致氧化应激减少。