Production of hypoxic adaptation model in rat

大鼠缺氧适应模型的制作

基本信息

批准号：
12558002
负责人：
OHKUWA Tetsuo
金额：
$ 7.36万
依托单位：
Nagoya Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

All experiments (hybrid, pregnancy, nurse and exercise) were carried out in the sterile, hypoxia chamber. At the age of 4 weeks, the Progenitor's group (PI) Wistar male and female rats were randomly assigned to the following groups : male rats : hypoxia and exercise (MHE), hypoxia and sedentary (MHS). The male and female exercising groups were allowed free access to the running wheel. The numbers of revolution of the wheel (1.16m, circumference) were recorded by using a mechanical counter that was read daily. Male and female rats who did the highest total amount of running distance during 8 weeks were selected. The selected male and female rats were hybridized. The children (the first of high-altitude native rats ; F1) were separated from their mother after postnatal day 21. The male rats of F1 were randomly divided into three groups : MHE-F1, MHS-F1 and normoxic and sedentary low-altitude native rats, as control group. At the age of 18 weeks, the P1, F1, F3 (the third high-altitude na … More tive rats), F5(the fifth) and F7(the seventh) were anesthetized by ether and sacrificed by sampling of blood from the aorta, and brain, heart, liver, diaphragm, skeletal muscle (soleus and gastrocnemius white muscle). The whole brain was divided into the following four regions : cerebellum, frontal lobe, striatum, hippocampus.Study (1) : Monoamines levels of the hypoxic exercise group in high-altitude native significantly decreased in the frontal lobe, cerebellum and striatum, compare with the other groups. These study suggest that hypoxic exercise decreased monoamine levels in whole brain, and that sensitivity to hypoxia and/or exercise differed according to brain regions.Study (2) No significant differences were found in the norepinephrine levels for the diaphragm and soleus muscle among the three groups. This study clarified that hypoxia elicits a decreased sympathetic response in the lover, However, it cause an increase in the response in the heart and gastrocnemius white muscle. These results suggest that the sympathetic response to long -term hypoxia changes with the rat tissues.Study (3) The present study confirmed that moderate hypoxia and hypoxic training induce attenuate the liver DNA damage and decrease the liver GSH level and GR activity. These results indicate that moderate hypoxia and hypoxic training result in deceased oxidative stress. Less

所有实验（混合，怀孕，护士和运动）均在无菌的缺氧室内进行。在4周的时候，将祖先（PI）Wistar雄性和雌性大鼠随机分配给以下组：雄性大鼠：缺氧和运动（MHE），缺氧和久坐不动（MHS）。允许男性和女性运动组自由进入跑步轮。通过使用每天读取的机械计数器记录车轮（116m，圆周）的革命数量。选择了在8周内进行总跑步距离最高的男性和女性大鼠。选定的雄性和雌性大鼠杂交。产后第21天后，儿童（高海拔天然大鼠的第一大鼠； F1）与母亲分离。F1的雄性大鼠被随机分为三组：MHE-F1，MHS-F1以及作为对照组的MHE-F1，MHS-F1和Normoxic和Normoxic和Normoxic和Normoxic和Normoxic and久坐的低海拔天然大鼠。在18周的年龄，P1，F1，F3（第三个高高度NA…更加较长的大鼠），F5（第五）和F7（第七）（第七个）通过以太进行麻醉，并通过主动脉，大脑，心脏，肝脏，肝脏，skeletal uscle uscle（Selemus and selemus and salemus and coscecle uscle ands serclious ands serclius sakcly and the sarde sar来牺牲血液。整个大脑都分为以下四个区域：小脑，额叶，纹状体，海马。（1）：高蓝色天然中低氧运动组的单胺水平在额叶，小脑和纹状体中显着降低，与其他组相比。这些研究表明，缺氧运动降低了整个大脑的单胺水平，并且根据大脑区域对缺氧和/或运动的敏感性。研究（2）在三组中，在去甲肾上腺素水平上发现了去甲肾上腺素水平的显着差异。这项研究澄清说，缺氧会导致情人的交感神经反应降低，但是，这会导致心脏和腹腔白色肌肉的反应增加。这些结果表明，对长期缺氧的交感神经反应随大鼠组织的变化。这些结果表明，中等缺氧和缺氧训练会导致死亡的氧化应激。较少的