Effect of aging and physical training on hydroxyl radical generation and scavenging capacity.

衰老和体能训练对羟自由基产生和清除能力的影响。

基本信息

批准号：
07680110
负责人：
OHKUWA Tetsuo
金额：
$ 1.41万
依托单位：
Nagoya Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

The effects of exercise on the generation of active oxygen species and radicalscavenging capacity were studied in physically active and sedentary young and old rats. Exercise increased the hydroxyl radical content in all tissues of physically active young rats, except in the plasma. In old rats, the basal level of the radical increased significantly in plasma, heart, and skeletal muscles, but decreased in liver ; and physical activity decreased it to that of young ones in most cases. With exercise, the content of reduced glutathione increased in plasma, heart, and skeletal muscles of young rats, whereas that of oxidized glutathione markedly decreased in liver and increased in brain and white gastrocnemius muscle. The total glutathione levels in these tissues changed in a similar way, indicating that glutathione was released from the pool in the liver. In rats allowed to run voluntarily for 5 weeks, the effects were more pronounced than in the sedentary ones. The ratio of reduced to total glutathione, which indicates the capacity to reduce glutathione, increased in plasma, heart, and soleus muscle of sedentary young rats after exercise, and increased further in those with the physical activity. In old rats, the reduced glutathione level increased in plasma, heart, liver, and brain, even though the total decreased. These results suggest that physical activity enhances the endogenous ability to defend against oxidative stress. In old rats, even though glutathione synthesis is decreased, the regenerating capacity seems to be increased in order to compensate for the increased oxidative stress.

研究了运动对活跃和久坐的年轻和年老大鼠活性氧生成和自由基清除能力的影响。运动增加了体力活跃的幼鼠所有组织中的羟自由基含量，血浆除外。在老年大鼠中，血浆、心脏和骨骼肌中自由基的基础水平显着升高，而肝脏中自由基的基础水平降低；在大多数情况下，体力活动会使其下降至年轻人的水平。随着运动的进行，幼鼠血浆、心脏和骨骼肌中还原型谷胱甘肽的含量增加，而肝脏中氧化型谷胱甘肽的含量明显减少，脑和白色腓肠肌中氧化型谷胱甘肽的含量增加。这些组织中的总谷胱甘肽水平也以类似的方式变化，表明谷胱甘肽从肝脏的库中释放出来。在允许自愿跑步 5 周的老鼠中，效果比久坐的老鼠更明显。运动后久坐的幼鼠的血浆、心脏和比目鱼肌中还原型谷胱甘肽与总谷胱甘肽的比率（表明还原型谷胱甘肽的能力）增加，并且在进行体力活动的大鼠中进一步增加。在老年大鼠中，血浆、心脏、肝脏和大脑中还原型谷胱甘肽的水平有所增加，尽管总量有所下降。这些结果表明体力活动增强了抵御氧化应激的内源能力。在老年大鼠中，尽管谷胱甘肽合成减少，但再生能力似乎有所增加，以补偿增加的氧化应激。