Analysis of genetic risk factors in periodontal diseses ― Effect of cellular aging on damage of cells ―

牙周病遗传危险因素分析―细胞衰老对细胞损伤的影响―

• 批准号: 12557178
• 负责人: HASEGAWA Tomokazu
• 金额: $ 6.85万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557178/
• 关键词: genetic risk factor; periodontal disease; Down syndrome; telomere DNA length; cellular aging; Interferon receptor; STAT1; Interleukin-6; 先天性リスクファクター; STA1; 特異的発現遺伝子; DNA chip; Microarray

1. Telomere DNA length of human fibroblasts derived from healthy and Down syndrome patients was analyzed by Southern blotting. After 5 times cell replication, it was shown that telomere DNA length was more short in fibroblasts from Down syndrome than from healty fibroblasts.2. Fibroblasts derived from Down syndrome patients expressed interferon alpha/ beta (IMF-α/β) receptor subunit, IFNAR1, stronger than fibroblasts from healthy patients.3. STAT1, one of transcriptional factors in the downstream of IMF receptor, was activated by the treatment of INF- γ. Western blotting demonstrated that activated STAT1 in Down fibroblasts was expressed stronger than in healthy fibroblasts after the treatment of INF-γ. However, in the presence of anti-INF-β antibody, activated STAT1 was reduced by the treatment of INF-γ.4. Interleukin-6 (IL-6), one of inflammatory cytokines, was induced by the treatment of INF -γ. ELISA demonstrated that IL-6 production in Down fibroblasts increased more abundantly than in healthy fibroblasts by the treatment of INF- γ.

1.Southern blotting分析健康人和唐氏综合症患者成纤维细胞的端粒DNA长度，经过5次细胞复制后发现，唐氏综合症成纤维细胞的端粒DNA长度比健康成纤维细胞短。2．源自唐氏综合症患者的成纤维细胞表达干扰素α/β(IMF-α/β)受体亚基IFNAR1，其表达强于健康患者的成纤维细胞。3． STAT1是已证实的IMF受体下游的转录因子之一，通过INF-γ处理而被激活，Western blotting显示，在INF-γ处理后，在Down成纤维细胞中激活的STAT1表达强于健康成纤维细胞。抗INF-β抗体的存在，活化的STAT1通过INF-γ.4的治疗而减少，IL-6是炎症细胞因子之一。 INF-γ处理表明，通过INF-γ处理，唐氏成纤维细胞中IL-6的产生量比健康成纤维细胞中增加得更多。

项目成果

Hasegawa, T. (et al.): "Expression of osteoprotegerin/osteoclastogenesis inhibitory factor and osteoprotegerin ligand/osteoclast differentiation factor in cultures of periodontal ligament cells derived from human permanent teeth"J. Periodontal Research. 3

Hasekawa, T.（等人）：“在源自人恒牙的牙周膜细胞培养物中保护骨素/破骨细胞生成抑制因子和骨保护素配体/破骨细胞分化因子的表达”J.

Hasegawa, T. (et al.): "Human periodontal ligament ells derived from deciduous teeth induce osteoclastogenesis In vitro"Tissue and Cell. 34 (in press). (2002)

Hasekawa, T.（等人）：“来自乳牙的人牙周韧带细胞在体外诱导破骨细胞生成”组织和细胞。

Hasegawa, T.: "Expression of osteoprotegerin/osteoclastogenesis inhibitory factor and osteoprotegerin ligand/osteoclast differentiation factor in cultures of periodontal ligament cells derived from human permanent teeth"Journal of Periodontal Research. 37

长谷川，T.：“来自人恒牙的牙周膜细胞培养物中骨保护素/破骨细胞生成抑制因子和骨保护素配体/破骨细胞分化因子的表达”牙周研究杂志。

Hasegawa, T.: "Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro"Tissue and Cell. 34(in press). (2002)

Hasekawa, T.：“源自乳牙的人牙周膜细胞在体外诱导破骨细胞生成”《组织与细胞》。