Biomedical approach of new materials with well-organized interfaces

具有组织良好的界面的新材料的生物医学方法

基本信息

批准号：
11694129
负责人：
KATAOKA Kazunori
金额：
$ 3.14万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

1) Nanostructure fabrication from block copolymers was carried out in this study. Particularly, novel approaches for the construction of functionalized poly(ethylene glycol) (PEG) layers on surfaces were focused to attain the specific adsorption of a target protein through a PEG-conjugated ligands with a minimal non-specific adsorption of other proteins. Further, surface organization of block copolymer micelles with cross-linking core was studied from a standpoint of the preparation of a new functional surface-coating with a unique macromolecular architecture. The micelle-attached surface and the thin hydrogel layer made by layered micelles exhibited nonfouling properties and worked as the reservoir for hydrophobic reagents. These PEG-functionalized surface in brush form or in micelle form can be used in diverse fields of medicine and biology to construct high-performance medical devices including scaffolds for tissue engineering and matrices for drug delivery systems.2) Recently, coll … More oidal carrier systems have been receiving much attention in the field of drug targeting because of their high loading capacity for drugs as well as of their unique disposition characteristics in the body. This research project was aimed at the utility of polymeric micelles formed through the multimolecular assembly of block copolymers as novel core-shell typed colloidal carriers for drug and gene targeting. The process of micellization in aqueous milieu is studied in detail based on the differences in the driving force of core segregation, including hydrophobic interaction, electrostatic interaction, metal complexation, and hydrogen bonding of constituent block copolymers. The segregated core embedded in the hydrophilic palisade is shown to function as a reservoir for genes, enzymes, and a variety of drugs with diverse characteristics. Functionalization of the outer surface of the polymeric micelle to modify its physicochemical and biological properties is investigated from the standpoint of designing micellar carrier systems for receptor-mediated drug delivery. Further, the body distribution of polymeric micelles is studied to demonstrate their long-circulating characteristics and significant tumor accumulation, emphasizing their promising utility in tumortargeting therapy. As an important perspective on carrier systems based on polymeric micelles, their feasibility as nonviral gene vectors is also demonstrated in this study. Less

1）在本研究中进行了块共聚物的纳米结构制造。特别是，以表面上的功能化聚（乙二醇）（PEG）层构建的新型方法集中在通过PEG偶联的配体中获得靶蛋白的特异性添加方法，其非特异性非特异性添加吸收其他蛋白质。此外，从制备具有独特的大分子结构的新功能表面涂层的角度，研究了具有交联芯的块共聚物胶束的表面组织。由分层胶束暴露于非冻结特性制成的胶束附着的表面和薄水凝胶层，并作为疏水试剂的储层。这些以刷子形式或胶束形式的PEG功能化的表面可以在医学和生物学的潜水领域中使用，以构建高性能的医疗设备，包括用于组织工程的脚手架和药物输送系统的脚手架。2）最近，Coll Coll Idal载体系统在药物范围内引起了更多的注意力，因为它们在药物范围内引起了人们对药物的高负荷能力的关注，因为它们的药物能力及其独特的特征及其独特的特征。该研究项目的目的是通过块共聚物的多分子组装形成的聚合物胶束，作为用于药物和基因靶向的新型核壳型胶体载体。根据核心隔离的驱动力（包括疏水相互作用，静电相互作用，金属络合物以及组成块共聚物的氢键），对水环中的胶束化过程进行了详细研究。嵌入在亲水性栅栏中的隔离核心被证明是基因，酶和各种具有不同特征的药物的储层。从设计用于受体介导的药物输送的胶束载体系统的角度研究了聚合物胶束外表面以修饰其物理和生物学特性的功能化。此外，研究了聚合物胶束的身体分布，以证明其长循环特征和大量肿瘤积累，强调了它们在肿瘤疗法中的承诺效用。作为基于聚合物胶束的载体系统的重要视角，本研究也证明了它们作为非病毒基因载体的可行性。较少的