Biomedical approach of new materials with well-organized interfaces

具有组织良好的界面的新材料的生物医学方法

基本信息

批准号：
11694129
负责人：
KATAOKA Kazunori
金额：
$ 3.14万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

1) Nanostructure fabrication from block copolymers was carried out in this study. Particularly, novel approaches for the construction of functionalized poly(ethylene glycol) (PEG) layers on surfaces were focused to attain the specific adsorption of a target protein through a PEG-conjugated ligands with a minimal non-specific adsorption of other proteins. Further, surface organization of block copolymer micelles with cross-linking core was studied from a standpoint of the preparation of a new functional surface-coating with a unique macromolecular architecture. The micelle-attached surface and the thin hydrogel layer made by layered micelles exhibited nonfouling properties and worked as the reservoir for hydrophobic reagents. These PEG-functionalized surface in brush form or in micelle form can be used in diverse fields of medicine and biology to construct high-performance medical devices including scaffolds for tissue engineering and matrices for drug delivery systems.2) Recently, coll … More oidal carrier systems have been receiving much attention in the field of drug targeting because of their high loading capacity for drugs as well as of their unique disposition characteristics in the body. This research project was aimed at the utility of polymeric micelles formed through the multimolecular assembly of block copolymers as novel core-shell typed colloidal carriers for drug and gene targeting. The process of micellization in aqueous milieu is studied in detail based on the differences in the driving force of core segregation, including hydrophobic interaction, electrostatic interaction, metal complexation, and hydrogen bonding of constituent block copolymers. The segregated core embedded in the hydrophilic palisade is shown to function as a reservoir for genes, enzymes, and a variety of drugs with diverse characteristics. Functionalization of the outer surface of the polymeric micelle to modify its physicochemical and biological properties is investigated from the standpoint of designing micellar carrier systems for receptor-mediated drug delivery. Further, the body distribution of polymeric micelles is studied to demonstrate their long-circulating characteristics and significant tumor accumulation, emphasizing their promising utility in tumortargeting therapy. As an important perspective on carrier systems based on polymeric micelles, their feasibility as nonviral gene vectors is also demonstrated in this study. Less

1) 本研究特别关注在表面构建功能化聚乙二醇 (PEG) 层的新方法，以通过嵌段共聚物制造纳米结构，以通过 PEG 缀合实现目标蛋白质的特异性吸附。此外，从制备具有独特功能的新型功能性表面涂层的角度研究了具有交联核心的嵌段共聚物胶束的表面组织。胶束附着的表面和由层状胶束制成的薄水凝胶层具有防污特性，可作为疏水性试剂的储存库，这些刷状或胶束形式的 PEG 功能化表面可用于医学和生物医学的各个领域。生物学来构建高性能医疗设备，包括用于组织工程的支架和用于药物输送系统的基质。2）最近，Coll … More 球形载体系统在药物靶向领域受到了广泛关注，因为该研究项目旨在利用嵌段共聚物多分子组装形成的聚合物胶束作为药物和基因的新型核壳型胶体载体。基于核心分离驱动力的差异，包括疏水相互作用、静电相互作用、金属络合和构成嵌段的氢键，详细研究了水环境中的胶束化过程。嵌在亲水栅栏中的分离核心被证明可以充当基因、酶和具有不同特性的各种药物的储存库，研究了聚合物胶束外表面的功能化以改变其物理化学和生物特性。设计用于受体介导的药物递送的胶束载体系统的观点此外，研究了聚合物胶束的体内分布，以证明其长循环特性和显着的肿瘤积累，强调了它们在治疗中的应用前景。作为基于聚合胶束的载体系统的一个重要视角，其作为非病毒基因载体的可行性在本研究中也得到了证明。