Development of contimous cell separation system using polymeric adsorbent with surface micro-structures

使用具有表面微结构的聚合物吸附剂开发连续细胞分离系统

基本信息

批准号：
05558118
负责人：
KATAOKA Kazunori
金额：
$ 3.33万
依托单位：
Science University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The interaction of rat lymphocyte subpopulations with the surfaces of poly (2-hydroxyethyl methacrylate) -graft-polyamine copolymer (HA) and poly (2-hydroxyethyl methacrylate) -graft-partially quaternized polyamine copolymer (HQA) was evaluated under a controlled shear stress by using a plane-parallel type channel equipped with a polymer-coated bottom wall. The cells introduced into the channel were settled for a prescribed time period, followed by the exposure to a shear stress resulting from the parabolic flow in the channel. When the shear stress exceeded the strength of cell/surface interaction, the cells were able to be detached from the polymer surface. By this way, quantitative evaluation of cell/surface interaction was done in time-dependent manner to clarify the effect of quaternization of polyamine portion in graft copolymer on lymphocyte attachment. Extended settling time (Tsf) caused a progressive increase in the attachment force between lymphocyte and copolymer surface for HA channel, resulting in a considerable decrease in the recovery of both B and T cells from the channel. On the contrary, recovery of T cells from HQA channel showed only a slight decrease with a settling time, allowing to obtain T cell population as effluent in a good yield even under the condition with prolonged settling time. Time-dependent increase in cell/surface interaction observed for HA channel might be due to a progressive contribution of hydrophobic interaction caused by an increase in hydrophobicity of HA copolymer through the deprotonation of ethylenediamine units in polyamine grafts at cellular interface. These results indicated that lymphocyte attachment is able to be controlled by changing the amount and the nature (fixed our unfixed) of charge in polyamine graft copolymers.

大鼠淋巴细胞亚群与聚（2-羟基乙基甲基丙烯酸酯） - 晶型聚胺共聚物（HA）和聚（2-羟基甲基丙烯酸甲基甲基甲基酯）的表面的相互作用聚合物涂层的底壁。引入通道中的细胞被定为规定的时间段，然后暴露于通道中的抛物线流动引起的剪切应力。当剪切应力超过细胞/表面相互作用的强度时，细胞可以从聚合物表面脱离。通过这种方式，以时间依赖性的方式对细胞/表面相互作用进行定量评估，以阐明聚胺部分在移植物共聚物中对淋巴细胞附着的偶四分化的影响。扩展的沉降时间（TSF）导致HA通道的淋巴细胞和共聚物表面之间的附着力逐渐增加，从而导致B和T细胞从通道的回收率大幅下降。相反，从HQA通道中恢复T细胞随着沉降时间仅略有减少，即使在延长的沉降时间下，即使在这种情况下，也可以使T细胞种群以良好的收率获得废水。对于HA通道观察到的细胞/表面相互作用的时间依赖性增加可能是由于疏水相互作用的逐渐贡献是由HA共聚物在细胞界面上多胺移植物中的乙二胺单位的疏水性增加而引起的。这些结果表明，淋巴细胞的附着可以通过改变多胺移植物共聚物中的量和性质（固定我们的未固定）来控制。