THE PRODUCTION OF THE NEW POLYAMIDE TYPE POLYMER MATERIALS FROM LIGNIN USING GENE RECOMBINATION OF MICRO ORGANISM

利用微生物基因重组从木质素生产新型聚酰胺类高分子材料

• 批准号: 11556030
• 负责人: KATAYAMA Yoshihiro
• 金额: $ 8.7万
• 依托单位: TOKYO UNIVERSITY OF AGRICULTURE & TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11556030/
• 关键词: liignin; biotransformation; Sptiingomoas paucimobilis SYK-6.; genelJc engineering; 2-Pyrone-4,6-dicarboxylicacid; bio-reactor; polyamidetype polymer; interfacialpolymerizatiou reaction; 2-ピロン4, 6-ジカルボン酸; バイオリアクター; 2-ピロン4,6-ジカルボン酸

This project has been investigating the biotransformation of lignin based on the catabolism of ligninolytic bacterium Sphing omonas paucimobilis SYK-6. 2-Pyroae-4 , 6-dicarboxylicacid (PDC) is a major and characteristic intermediate of protocatecuate meta cleavage pathway of Sphinomonas paucimobilis SYK-6 in lignin degradation. We have preparedthe new recombinant plasmid vector which was based on the Escherichia coli.-Pseudomonas shutU.5 vector PKT-230 and composed of Lig A, B genes (protocatechuate 4,4-dioxygenase gene), the modified VanA, B gene (Vanillate, Syringate demethylase gene) and Lig C gene (PDC synthase). In this project research, we have succeeded in producing PDC in the high yield (ca.90%) from the low-molecular weight lignin by the transformant of Pseodoaiowsputicla PpYllOO having the new reconbinantplasmid vector.Since PDC has the unique molecular structure and the polymer made froin PDC is expectedto have the new function as optical material, we prepared new polyamide type polymer material by the inteifacial polymerizationreaction with hexamethylenediamine.

该项目一直在研究木质素分类细菌的分解代谢Omonas paucimobilis syk-6的生物转化。 2-Pyroae-4，6-二羧酸（PDC）是木质素降解中paucimobilis syk-6的prodocatecuate元裂解途径的主要且特征的中间体。我们已经准备了新的重组质粒载体，该质粒载体基于大肠杆菌 - 假单胞菌shutu.5载体PKT-230，由LIG A，B基因（Protocatechuate 4,4-二氧酶基因），修改后的VANA，B基因，B基因（vanillate，Anillate，nimenate demethys Gene）和lig conne conte conte conte contem contem contem and b gene（Protocatechuate 4,4-二激酶基因）。在这项项目的研究中，我们成功地从低分子重量木质素中以高产量（Ca.90％）生产了Pseodoaiowoaiowsputicla ppylloo具有新的recombinantplasmid载体的ppyoaiowsptipicla ppylloo。六甲基二胺的深度聚合反应。

项目成果

Masai,E., Katayama,Y., Nishikawa,S., Fukuda,M.: "Characterization of Sphingomonas paucimobilis SYK-6 genes involved in degradation of lignin-related compounds"Journal of Industrial Microbiology & Biotechnology. 23. 364-373 (1999)

Masai,E.、Katayama,Y.、Nishikawa,S.、Fukuda,M.：“少动鞘氨醇单胞菌 SYK-6 基因参与木质素相关化合物降解的特征”工业微生物学杂志

Nishikawa S., Shigehara K., Kalayama Y.: "Hie development of the new polyamide material.-The production of the nylon with biodegradability from lignin-"Chemistry and Chemical Industry (Japanese). 52(8). 988-991 (1999)

Nishikawa S.、Shigehara K.、Kalayama Y.：“新型聚酰胺材料的开发。-用木质素生产具有生物降解性的尼龙-”化学和化学工业（日语）。

Hara,F.,Masai,E.,Katayama,Y.Fukuda,M,: "The 4-Oxalomesaconate Hydratase Gene, Involved in the Protocatechuate 4, 5-CleavagePathway, Is Essential to Vanillate and Syringate Degradation in Sphingomonas paucimobilis SYK-6"J.Bac.. 24. 6950-6957 (2000)

Hara,F.,Masai,E.,Katayama,Y.Fukuda,M,：“参与原儿茶酸 4, 5-裂解途径的 4-草酰中康酸水合酶基因对于少动鞘氨醇单胞菌 SYK-6 中香草酸和注射器降解至关重要

Sonoki K., Ohtsuka Y., Ikeda S., Masai E., Kajita K., Katayama, Y: "Close Association between the Enzymeslnvolved in the Ligin Metabolic Pathway of SphingomonapaucimobilisSYK-6"Journal of Wood Science. (印刷中). (2002)

Sonoki K.、Ohtsuka Y.、Ikeda S.、Masai E.、Kajita K.、Katayama, Y：“参与鞘氨醇单胞菌 SYK-6 木质素代谢途径的酶之间的密切关联”《木材科学》杂志（正在出版）。 (2002)

Sonoki,K., Ohtsuka, Y., Ikeda,S.,Masai, E., Kajita,K., Katayama, Y: "Close Association between the Enzymes Involved in the Lignin Metabolic Pathway of Sphingomonas paucimobilis SYK-6"Journal of Wood Science. 48. 250-252 (2002)

Sonoki,K.、Ohtsuka, Y.、Ikeda,S.、Masai, E.、Kajita,K.、Katayama, Y：“少动鞘氨醇单胞菌 SYK-6 木质素代谢途径中涉及的酶之间的密切关联”杂志