Analysis of expression pattern of the gene which is related to the radiation sensitivity in urothelial cancer and its clinical application

尿路上皮癌放射敏感性相关基因表达模式分析及临床应用

基本信息

批准号：
15390482
负责人：
SHINOHARA Nobuo
金额：
$ 5.12万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

From the present project, the following results were obtained.1.Initially, we evaluated radiation sensitivity in the nude mice transplanted with human bladder cancer cell lines. Utilizing this model, we set up the radiation exposure condition (single time irradiation of 5 Gy) and confirmed that tumor growth delayd reaction could be evaluated as an endpoint of radiation sensitivity.2.The establishment of Xenogarft model : Tumor growth was observed in 18 mice (60%, 18/30). Histologically, 15 of the18 were epithelial carcinomas similar to the original tumors, resulting in a 50% (15/30) take rate. No correlation was found between the tumor take rate and the clinicopathologic features, TP53 mutational status, or Ki67 LI of the patients' tumors. Of the 15 xenografts, 11 xenografts were passed from 3 to 10 generations. TP53 mutational status remained stable during the passages, though the Ki67 LI slightly increased in the majority of xenografts. Specific growth delay after irradiation in the 4 xenografts was observed independent of the original tumor growth speed and Ki67 LI.3.Radiological growth delay assay : To assess the usefulness of these xenografts for therapeutic simulation, we performed radiological growth-delay assay in 4 xenografts and examined the relation between the responses and the biological parameters of xenograft tumors. The radiation response considerably varied among the 4 models. Absolute growth delay ranged from 20.3+2.4 (mean+standard error) to 76.4±7.4 days. Specific growth delay (SGD) was not associated with in vivo tumor growth speed or the Ki67 Il. The smallest SGD value was observed in the No.3 model which harbored TP53 gene mutation.

从本项目中获得以下结果。1。在本质上，我们评估了用人膀胱癌细胞系移植的裸鼠中的辐射敏感性。利用该模型，我们设置了辐射暴露条件（单个时间照射为5 Gy），并确认可以将肿瘤生长延迟反应评估为辐射敏感性的终点。2。Xenogarft模型的建立：在18个鼠标中观察到肿瘤的生长（60％，18/30）。从组织学上讲，这18个中的15个是类似于原始肿瘤的上皮癌，导致50％（15/30）的速率。在肿瘤接受率与临床病理学特征，TP53突变状态或患者肿瘤的Ki67 LI之间没有发现相关性。在15个Xenographictic中，有11张Xenographictics从3到10代中传递。 TP53突变状态在通道期间保持稳定，尽管大多数异种移植物中的Ki67 li略有增加。观察到4种异种移植后的特定生长延迟独立于原始肿瘤生长速度和Ki67 li.3.-Fradiologoly延迟测定法：为了评估这些异种移植物在治疗模拟中的有用性，我们在4个内属移植物中进行了放射线生长 - 延迟分析，并检查了Xeneraft和Biological Parmeters the Biologic parameraft之间的关系。这4个模型之间的辐射响应差异很大。绝对生长延迟范围从20.3+2.4（平均+标准误差）到76.4±7.4天。特异性生长延迟（SGD）与体内肿瘤生长速度或Ki67 IL无关。在藏有TP53基因突变的第3号模型中观察到了最小的SGD值。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Prediction of lymph node metastasis by analysis of gene expression profiles in non-small cell lung cancer.

DOI：
10.1016/j.jss.2004.06.002
发表时间：
2004-11
期刊：
The Journal of surgical research
影响因子：
0
作者：
M. Takada;M. Tada;Eiji Tamoto;Akiko Kawakami;Katsuhiko Murakawa;G. Shindoh;Ken-ichi Teramoto;A. Matsunaga;K. Komuro;M. Kanai;Y. Fujiwara;K. Shirata;Norihiro Nishimura;M. Miyamoto;S. Okushiba;S. Kondo;J. Hamada;H. Katoh;T. Yoshiki;T. Moriuchi
通讯作者：
M. Takada;M. Tada;Eiji Tamoto;Akiko Kawakami;Katsuhiko Murakawa;G. Shindoh;Ken-ichi Teramoto;A. Matsunaga;K. Komuro;M. Kanai;Y. Fujiwara;K. Shirata;Norihiro Nishimura;M. Miyamoto;S. Okushiba;S. Kondo;J. Hamada;H. Katoh;T. Yoshiki;T. Moriuchi

進行性尿路上皮癌に対するTIN(Paclitaxel, Ifosfamide, Nedaplatin)療法

TIN（紫杉醇、异环磷酰胺、奈达铂）治疗晚期尿路上皮癌