Development of ESR・MRI Dual Imager for Visualization of Reactive Oxygens

开发用于活性氧可视化的 ESR/MRI 双成像仪

基本信息

批准号：
15390363
负责人：
FUJII Hirotada
金额：
$ 8.38万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390363/
关键词：
visualization MRI ESR active oxygen free radical superoxide 活性酵素画像化

项目摘要

Electron Paramagnetic Resonance Imaging (EPRI) technique has been developed to obtain the spatial distribution of the paramagnetic materials, particularly on a microscopic scale. A large number of biological studies were successfully carried out, especially for the visualization of bio-radicals generated in in vivo system. Typical example is the detection and visualization of nitric oxide (NO) in septic-shocked animals. The strength of EPRI is that only free radicals are visible in its image. On the contrary, weakness of EPRI is that nothing can be visible except paramagnetic materials on its image. Therefore, it seems very hard to obtain in which organs the bioradicals were generated in the small animals. In order to overcome this problem, there are three possible methods to visualize free radicals on the anatomical map of the object. 1: PEDRI (Proton Electron Double Resonance Imaging), 2: conventional MRI, and 3: EPR+MRI system. In this study, we have tried developing EPR/MRI dual imaging system to visualize free radicals on the anatomical map of the object.EPR/MRI dual imaging system was constructed by using the permanent magnet (Nb-Fe-B material) rather than electromagnets or superconductive magnets because it is easier to assemble and combine EPR and MRI components. EPR spectrometer in this dual imaging system is working at 1.2 GHz at 0.04T, and MRI spectrometer in this dual system is working at 22 MHz at 0.5T. The most surprising feature is that the center of MRI magnet is only 20 cm apart from that of EPR magnet. Each spectrometer is working well without any magnetic or electric interference.

电子顺磁共振成像 (EPRI) 技术已被开发用于获取顺磁性材料的空间分布，特别是在微观尺度上。大量的生物学研究已成功开展，特别是体内系统中产生的生物自由基的可视化。典型的例子是感染性休克动物中一氧化氮 (NO) 的检测和可视化。 EPRI 的优势在于其图像中仅可见自由基。相反，EPRI 的弱点是图像上除了顺磁材料之外什么都看不见。因此，似乎很难获知小动物的生物自由基是在哪些器官中产生的。为了克服这个问题，存在三种可能的方法来在对象的解剖图上可视化自由基。 1：PEDRI（质子电子双共振成像），2：传统MRI，3：EPR+MRI系统。在本研究中，我们尝试开发 EPR/MRI 双成像系统，以在对象的解剖图上可视化自由基。EPR/MRI 双成像系统是使用永磁体（Nb-Fe-B 材料）而不是电磁体构建的或超导磁体，因为它更容易组装和组合 EPR 和 MRI 组件。该双成像系统中的EPR光谱仪工作在1.2 GHz、0.04T，而该双系统中的MRI光谱仪工作在22 MHz、0.5T。最令人惊讶的特点是MRI磁体的中心与EPR磁体的中心相距仅20厘米。每个光谱仪都工作良好，没有任何磁或电干扰。