New visualization technique of radiation effects on cancers

辐射对癌症影响的新可视化技术

• 批准号: 13670956
• 负责人: FUJII Hirotada
• 金额: $ 2.3万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670956/
• 关键词: free radicals; non-invasive visualization; ESR; oxygen; oximetry

It would be very desirable to be able to measure directly the oxygen tension in tissues to assess their state and response to therapy. This would be especially desirable for planning and evaluating the radiation therapy for tumors and vascular insufficiencies.The wide variety of techniques for measuring oxygen tensions in biological systems have been reviewed, but one of these methods has been demonstrated for the clinical use. Several NMR techniques have been studied recently, especially the use of an oxygen-dependent proton hyperfine line in myoglobin and oxygen-dependent relaxation of 19F. However, these approaches have not been demonstrated to have sufficient sensitivity and applicability for the real clinical use.Electron paramagnetic resonance (EPR) oximetry is a technique which is based on the effect of molecular oxygen on the line width of EPR spectra of some paramagnetic materials.In this project we used glucose char as the paramagnetic materials and examined its following characterizations : accuracy, sensitivity, ease to use, toxicity, and stability in vivo. From our results, the glucose char appears to have the very good properties than other reported paramagnetic materials.The EPR oximetry using the glucose char will be promising method to measure oxygen tensions in vivo.

非常希望能够直接测量组织中的氧张力以评估它们的状态和对治疗的反应。这对于规划和评估肿瘤和血管功能不全的放射治疗来说是特别理想的。 已经对测量生物系统中氧张力的各种技术进行了综述，但其中一种方法已被证明可用于临床。最近研究了几种NMR技术，特别是肌红蛋白中氧依赖性质子超细谱线的使用和19F的氧依赖性弛豫。然而，这些方法尚未被证明对实际临床应用具有足够的灵敏度和适用性。电子顺磁共振（EPR）血氧定量法是一种基于分子氧对某些顺磁材料的EPR光谱线宽的影响的技术在这个项目中，我们使用葡萄糖炭作为顺磁性材料，并检查了其以下特性：准确性、灵敏度、易用性、毒性和体内稳定性。从我们的结果来看，葡萄糖炭似乎比其他报道的顺磁材料具有非常好的特性。使用葡萄糖炭的 EPR 血氧测定法将是测量体内氧张力的有前途的方法。

项目成果

Fujii H., Berliner L.J., Yoshikawa: "In vivo fate of paramagnetic iron oxide during sepsis"Mag.Reson.Imaging. 20. 271-276 (2002)

Fujii H.、Berliner L.J.、Yoshikawa：“败血症期间顺磁性氧化铁的体内命运”Mag.Reson.Imaging。

Fujii, H. et al.: "In Vivo Fate of Superparamagnetic Iron Oxides During Sepsis"Magnetic Resonance Imaging. 20(印刷中). (2002)

Fujii, H. 等人：“脓毒症期间超顺磁性氧化铁的体内命运”磁共振成像 20（出版中）。

Fujii H.et al.: "Consequences of nitric oxide generation in epileptic-seizure rodent models as studied by in vivo EPR"Magn.Reson.Med.. 48. 1051-1056 (2002)

Fujii H.等人：“通过体内 EPR 研究的癫痫发作啮齿动物模型中一氧化氮生成的后果”Magn.Reson.Med.. 48. 1051-1056 (2002)

Fujii H.et al.: "In vivo fate of superparamagnetic iron oxides during sepsis"Magn.Reson.Imaging. 20. 271-276 (2002)

Fujii H.等人：“败血症期间超顺磁性氧化铁的体内命运”Magn.Reson.Imaging。