Studies on new molecular therapeutic approach for radiation pneumonitis

放射性肺炎分子治疗新方法的研究

基本信息

批准号：
15390256
负责人：
SONE Saburo
金额：
$ 7.55万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390256/
关键词：
radiation pneumonitis CD13 aminopeptidase N macrophages bestatin Aminopeptidase N

项目摘要

The cell-surface glycoprotein CD13 first identified in leukocytes of the myeloid series is identical to aminopeptidase N (EC 3.4.11.2). In this study, we examined here the significance of CD13/aminopeptidase N in radiation-induced pulmonary fibrosis using an animal model. The activity of aminopeptidase in bronchoalveolar lavage fluid (BALF) was significantly higher in rats with radiation pneumonitis at 2-4 weeks after the irradiation than in control rats. CD13/aminopeptidase N protein, which has a molecular mass of approximately 150 kD, was detectable in alveolar macrophages (AM) from rats with radiation pneumonitis at higher levels than in those from control rats. Higher chemotactic activity for lymphocytes was detected in the BALF from rats with radiation pneumonitis than that from control rats, and the activity was significantly decreased by the treatment with bestatin, a specific aminopeptidase inhibitor, indicating that CD13/aminopeptidase N expressed in AM may have a role in T lymphocyte involvement in radiation pneumonitisn and the pathogenesis of alveolitis in this disorder. Our results suggest that bestatin may be useful for the treatment of radiation pneumonitis. We are now testing the effect of oral administration of bestatin in animal model of radiation pneumonitis.

在髓样系列的白细胞中首次鉴定的细胞表面糖蛋白CD13与氨基肽酶N相同（EC 3.4.11.2）。在这项研究中，我们在这里检查了CD13/氨基肽酶N在辐射诱导的肺纤维化中使用动物模型的重要性。在辐射后2-4周患有放射性肺炎的大鼠，支气管肺泡灌洗液（BALF）中氨基肽酶的活性明显高于对照大鼠。 CD13/氨基肽酶N蛋白的分子质量约为150 kD，在肺泡巨噬细胞（AM）中可检测到与对照大鼠相比，患有辐射肺炎的大鼠的肺泡巨噬细胞（AM）可检测到。 Higher chemotactic activity for lymphocytes was detected in the BALF from rats with radiation pneumonitis than that from control rats, and the activity was significantly decreased by the treatment with bestatin, a specific aminopeptidase inhibitor, indicating that CD13/aminopeptidase N expressed in AM may have a role in T lymphocyte involvement in radiation pneumonitisn and the pathogenesis of这种疾病中的肺泡炎。我们的结果表明，Bestatin可能对辐射肺炎的治疗可能很有用。现在，我们正在测试口服肺炎动物模型中Bestatin的作用。