Identification of membrane proteins participating myogenesis

参与肌生成的膜蛋白的鉴定

• 批准号: 15390089
• 负责人: SEHARA Atsuko
• 金额: $ 8.83万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390089/
• 关键词: Myogenesis; cellular differentiation; si RNA; GFP; membrane proteins; 組織形成; 骨格筋; 形態形成; 細胞増殖; siRNA

Coordinated development of multi-cellular organisms depends on intercellular communications and adhesions. Our research has been focused on identification of membrane proteins participating such cell-cell interactions during myogenesis. Inititially, we made a cDNA library of mRNA prepared from fusing muscle cells, which was subtracted with mRNA of myoblasts. From the screening of this library, we could identify a couple of novel membrane proteins. We are now trying to know functions of these proteins in myogenesis. We also succeeded to isolate a number of monoclonal antibodies from fusing muscle cells. Finally we studied roles of ADAM proteases in myogenesis, which we had cloned as membrane proteins activated by myogenic transcription factors. Based on the analyses of Meltrin α -/- and Meltrin β -/- mice, we concluded that these ADAM proteins indeed participate in myogenesis in vivo.

多细胞生物体的协调发育依赖于细胞间通讯和粘附，我们的研究重点是鉴定膜参与蛋白，例如肌生成过程中的细胞间相互作用。通过对这个文库的筛选，我们可以鉴定出一些新的膜蛋白。我们现在正在尝试了解这些蛋白在肌生成中的功能。我们还成功地分离出了一些单克隆抗体。最后，我们研究了 ADAM 蛋白酶在肌生成中的作用，我们将其克隆为由肌生成转录因子激活的膜蛋白，基于 Meltrin α -/- 和 Meltrin β -/- 小鼠的分析，我们得出结论： ADAM 蛋白确实参与体内的肌生成。

项目成果

Watabe-Uchida M.: "A Novel Metalloprotease-Disintegrin, Meltrin e (ADAM35), Expressed in Epithelial Tissues during Chick Embryogenesis"Dev.Dynamics. (in press).

Watabe-Uchida M.：“一种新型金属蛋白酶解整合素，Meltrin e (ADAM35)，在鸡胚胎发生过程中在上皮组织中表达”Dev.Dynamics。

Kurohara, K.: "Essential Roles of Meltrinβ / ADAM19 in Heart Development"Developmental Biol.. 267. 14-28 (2004)

Kurohara, K.：“Meltrinβ / ADAM19 在心脏发育中的重要作用”Developmental Biol.. 267. 14-28 (2004)

Wakatsuki S.: "Lipid rafts identified as locations of ectodomain shedding mediated Meltrin β/ADAM19"J.Neurochemistry. (in press).

Wakatsuki S.：“脂筏被确定为胞外域脱落介导的 Meltrin β/ADAM19 的位置”J.Neurochemistry（出版中）。

Roles of Meltrin α (ADAM12) in obesity induced by hish-fat diet.

Meltrin α (ADAM12) 在高脂肪饮食引起的肥胖中的作用。

• 发表时间: 2005
• 期刊: Endocrinology 146(4)
• 作者: Masaki;M.;et al.
• 通讯作者: et al.

Roles of Meltrin α (ADAM12) in obesity induced by high-fat diet.

Meltrin α (ADAM12) 在高脂饮食引起的肥胖中的作用。

• 发表时间: 2005
• 期刊: Endocrinology 146(4)
• 作者: Masaki;M.;et al.
• 通讯作者: et al.