Molecular signaling mechanisms underlying cardiovascular and branchial morphogenesis

心血管和鳃形态发生的分子信号机制

• 批准号: 14370231
• 负责人: KURIHARA Hiroki
• 金额: $ 9.54万
• 依托单位: The University of Tokyo (2003)Kumamoto University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370231/
• 关键词: neural crest cells; cardiovascular system; branchial arch; endothelin; gene-manipulated mouse; cell differentiation; transcription factor; morphogenesis; 心大血管形成; パターン形成; 分化制御; 血管平滑筋; 胚発生

1.Endothelin-1 (ET-1) has emerged as a key factor that regulates dorsoventral branchial arch patterning through the induction of Dlx5/6 homoebox transcription factors, thereby ET-1 appears to be involved in basic program of morphogenesis in this region.2.dHAND, a basic-helix-loop-helix transcription factor downstream to the ET-1 signaling, proved to be involved in the intermediate transition of vascular smooth muscle differentiation.3.Calpain6 has been identified as a factor downstream to the ET-1/dHAND pathway, which may participate in cell morphogenesis and proliferation by modification of cytoskeleton.4.TAZ has been identified as a transcriptional coactivator of Pax3, a Paired-type transcription factor involved in neural crest development.5.Transgenic mice in which EGFP and TVA (a receptor for avian retrovirus) expression are driven by the ETA receptor gene promoter has been established and served as a useful tool for researches on the cardiovascular system and neural crest development.

1.内皮素-1（ET-1）已成为通过诱导Dlx5/6同源盒转录因子调节背腹鳃弓模式的关键因子，因此ET-1似乎参与了该区域形态发生的基本程序。 2.dHAND是ET-1信号下游的碱性螺旋环螺旋转录因子，被证明参与血管平滑肌的中间转变3.Calpain6已被鉴定为ET-1/dHAND通路下游因子，可能通过细胞骨架的修饰参与细胞形态发生和增殖。4.TAZ已被鉴定为Pax3（一种配对- 5.建立了由ETA受体基因启动子驱动EGFP和TVA（禽逆转录病毒受体）表达的转基因小鼠，并作为神经嵴发育的转录因子。研究心血管系统和神经嵴发育的有用工具。

项目成果

Yamaguchi Y: "Identification of multiple novel epididymis-specific -defensin isoforms in humans and mice"J.Immunol.. 169. 2516-2523 (2002)

Yamaguchi Y：“人类和小鼠中多种新型附睾特异性防御​​素亚型的鉴定”J.Immunol.. 169. 2516-2523 (2002)

Nishimatsu H: "Role of endogenous adrenomedullin in the regulation of vascular tone and ischemic renal injury : studies on transgenic/knockout mice of adrenomedullin gene."Circ.Res.. 90. 657-663 (2002)

Nishimatsu H：“内源性肾上腺髓质素在调节血管张力和缺血性肾损伤中的作用：对肾上腺髓质素基因转基因/敲除小鼠的研究。”Circ.Res.. 90. 657-663 (2002)

Nishimatsu H: "Role of endogenous adrenomedullin in the regulation of vascular tone and ischemic renal injury : studies on transgenic/knockout mice of"Circ.Res.. 90. 657-663 (2002)

Nishimatsu H：“内源性肾上腺髓质素在调节血管张力和缺血性肾损伤中的作用：对转基因/基因敲除小鼠的研究”Circ.Res.. 90. 657-663 (2002)

Ito T: "Inhibitory effect of natriuretic peptides on aldosterone synthase gene expression in cultured neonatal rat cardiocytes."Circulation.. 107. 807-810 (2003)

Ito T：“利钠肽对培养的新生大鼠心肌细胞中醛固酮合酶基因表达的抑制作用。”Circulation.. 107. 807-810 (2003)

Ieda M: "Endothelin-1 regulates cardiac sympathetic innervation in the rodent heart by controlling nerve growth factor expression."J.Clin.Invest.. 113. 876-884 (2004)

Ieda M：“Endothelin-1 通过控制神经生长因子的表达来调节啮齿类动物心脏的交感神经支配。”J.Clin.Invest.. 113. 876-884 (2004)