Biological, Epidemiological and Clinical Studies on Amebic Infection among Institutionalized Populations

收容人群中阿米巴感染的生物学、流行病学和临床研究

• 批准号: 14370085
• 负责人: TAKEUCHI Tsutomu
• 金额: $ 7.23万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370085/
• 关键词: Entamoeba histolytica; Entamoeba dispar; amebiasis; infection among institutional population; axenic cultivation; genetic polymorphism; 赤痢アメーバ症; 無症候性感染; 赤痢アメーバ; 病原性; Entasmoeba dispar; Emtamoeba dispar; Entamoeba histolytica; Entamoeba dispar; amebiasis; infection among institutional population; axenic cultivation; genetic polymorphism; 赤痢アメーバ症; 無症候性感染; 赤痢アメーバ; 病原性; Entasmoeba dispar; Emtamoeba dispar

Epidemiological and clinical studies were attempted for residents in institutions, who shoed high infection rates with Entamoeba histolytica. Biological analyses were also conducted on the ameba isolates from such individuals. The epidemiological analyses demonstrated as high as 30% positive rate by the specific antigen detection method and the conventional stool examination. However, most of the infected seemed to be due to the persistent asymptomatic infections with E.histolytica. Several strains of E.histolytica isolated from such individuals with the persistent infection showed genetic and antigenic properties consistent with those of E.histolytica ; however, they did show virtually no virulence against tissue-cultured cells as examined with a sensitive fluorescent method. Based on the base sequence polymorphism of 4 different genes like chitinase gene, identification of genetic types of subpopulations of E.histolytica was developed, and applied to the isolates from several institu … More tions. The analyses demonstrated that the isolates from a single institution showed entirely the same genetic type, suggesting that only single infected individual is responsible for expansion of the amebic infection.Further studies indicated that the gene type at a few institutions with rather high infection rates was exactly the same as that detected in the fist outbreak in Kanagawa Prefecture, and demonstrated that an infected individuals had moved from the institute in Kanagawa to these new institutions. This method was also applied to the ameba isolates from foreign countries lice Thailand. However, virtually no coincidence was detected between these foreign isolates and domestic strains. Trials of axenic cultivation of Entamoeba dispar, nonpathogenic ameba, was also actively conducted, which finally resulted in a newly designed YIGADHA-S medium, which allowed complete axenic cultivation of this ameba. Biological characterization of ameba isolates was also attempted by examining the process of excystation and sulfur containing amino acid metabolism, which resulted in discovery of several novel inhibitors. The possibility of these inhibitors as lead compounds for drug development will be investigated. The experimental model for abscess formation was produced by using a Syrian golden hamster cheek pouch. Less

试图为居民机构进行流行病学和临床研究，这些机构使用Entamoeba Histolytica散发出高感染率。还对此类个体的Ameba分离株进行了生物分析。通过特定的抗原检测方法和常规粪便检查，流行病学分析证明了高达30％的正率。然而，大多数感染似乎是由于e.hastolotica的持续不对称感染。从具有持续感染的个体中分离出的几种菌株，显示了与E.hastolodica一致的遗传和抗原特性。但是，它们确实显示出用敏感荧光法检查的组织培养细胞对组织培养细胞的病毒。基于4种不同基因（例如几丁质酶基因）的碱基序列多态性，开发了遗传性亚群的遗传类型的鉴定，并应用于来自多个机构的分离株……更多。 The analyses demonstrated that the isolates from a single institution showed entirely the same genetic type, suggesting that only single infected individual is responsible for expansion of the amebic infection.Further studies indicated that the gene type at a few institutions with rather high infection rates was exactly the same as that detected in the fist outbreak in Kanagawa Prefecture, and demonstrated that an infected individuals had moved from the institute in Kanagawa to these new机构。该方法还应用于来自外国泰国虱子的阿米巴分离株。但是，这些外国分离株和家庭菌株之间几乎没有发现巧合。还积极地进行了非对病毒性ameba的Entamoeba Dispar的轴承培养的试验，最终导致了新设计的Yigadha-S培养基，该培养基允许对此Ameba进行完整的轴承培养。 Biological characterization of ameba isolates was also attempted by examining the process of excystation and sulfur containing amino acid metabolism, which resulted in discovery of several novel inhibitors.这些抑制剂作为药物开发的铅化合物的可能性将被研究。脓肿形成的实验模型是通过使用叙利亚金仓鼠脸颊袋产生的。较少的