Biological, Epidemiological and Clinical Studies on Amebic Infection among Institutionalized Populations

收容人群中阿米巴感染的生物学、流行病学和临床研究

• 批准号: 14370085
• 负责人: TAKEUCHI Tsutomu
• 金额: $ 7.23万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370085/
• 关键词: Entamoeba histolytica; Entamoeba dispar; amebiasis; infection among institutional population; axenic cultivation; genetic polymorphism; 赤痢アメーバ症; 無症候性感染; 赤痢アメーバ; 病原性; Entasmoeba dispar; Emtamoeba dispar

Epidemiological and clinical studies were attempted for residents in institutions, who shoed high infection rates with Entamoeba histolytica. Biological analyses were also conducted on the ameba isolates from such individuals. The epidemiological analyses demonstrated as high as 30% positive rate by the specific antigen detection method and the conventional stool examination. However, most of the infected seemed to be due to the persistent asymptomatic infections with E.histolytica. Several strains of E.histolytica isolated from such individuals with the persistent infection showed genetic and antigenic properties consistent with those of E.histolytica ; however, they did show virtually no virulence against tissue-cultured cells as examined with a sensitive fluorescent method. Based on the base sequence polymorphism of 4 different genes like chitinase gene, identification of genetic types of subpopulations of E.histolytica was developed, and applied to the isolates from several institu … More tions. The analyses demonstrated that the isolates from a single institution showed entirely the same genetic type, suggesting that only single infected individual is responsible for expansion of the amebic infection.Further studies indicated that the gene type at a few institutions with rather high infection rates was exactly the same as that detected in the fist outbreak in Kanagawa Prefecture, and demonstrated that an infected individuals had moved from the institute in Kanagawa to these new institutions. This method was also applied to the ameba isolates from foreign countries lice Thailand. However, virtually no coincidence was detected between these foreign isolates and domestic strains. Trials of axenic cultivation of Entamoeba dispar, nonpathogenic ameba, was also actively conducted, which finally resulted in a newly designed YIGADHA-S medium, which allowed complete axenic cultivation of this ameba. Biological characterization of ameba isolates was also attempted by examining the process of excystation and sulfur containing amino acid metabolism, which resulted in discovery of several novel inhibitors. The possibility of these inhibitors as lead compounds for drug development will be investigated. The experimental model for abscess formation was produced by using a Syrian golden hamster cheek pouch. Less

对溶组织内阿米巴感染率较高的机构居民进行了流行病学和临床研究，并对这些个体的阿米巴分离株进行了生物学分析，通过特异性抗原检测方法，流行病学分析显示阳性率高达30%。然而，大多数感染者似乎是由于溶组织内阿米巴的持续无症状感染所致。持续感染的个体表现出与溶组织内阿米巴一致的遗传和抗原特性；然而，根据 4 个不同基因的碱基序列多态性检测，它们实际上对组织培养细胞没有毒力。几丁质酶基因，开发了溶组织内阿米巴亚群的遗传类型鉴定，并将其应用于来自多个机构的分离株。分析表明，来自单个机构的分离株表现出完全相同的结果。进一步的研究表明，少数感染率较高的机构的基因型与神奈川县首次暴发时检测到的基因型完全一致，并且感染者从神奈川县的研究所转移到了这些新的机构，这种方法也适用于来自泰国的外国虱子菌株，但实际上没有发现这些外国菌株与泰国国内菌株之间存在重合。还积极进行了迪斯帕内阿米巴（非致病性阿米巴原虫）的无菌培养，最终产生了新设计的 YIGADHA-S 培养基，该培养基允许对该阿米巴原虫进行完全的无菌培养，并通过检查阿米巴分离物的过程和过程进行了尝试。含硫氨基酸代谢，从而发现了几种新型抑制剂，将研究这些抑制剂作为药物开发的先导化合物的可能性。使用叙利亚金仓鼠颊囊制作而成。

项目成果

Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area

• DOI: 10.1128/jcm.40.11.4081-4090.2002
• 发表时间: 2002-11-01
• 期刊: JOURNAL OF CLINICAL MICROBIOLOGY
• 影响因子: 9.4
• 作者: Haghighi, A;Kobayashi, S;Nozaki, T
• 通讯作者: Nozaki, T

竹内 勤, 小林正規, ほか: "寄生虫の院内(施設内)感染対策"厚生労働省医薬局安全対策課;メジカルフレンド社. 184 (2003)

Tsutomu Takeuchi、Masanori Kobayashi 等：“医院内（设施）寄生虫感染的对策”厚生劳动省制药药房安全科第 184 号（2003 年）

Inhibition of excystation and metacystic development of Entamoeba invadens by the dinitroanilin herbicide oryzalin

二硝基苯胺除草剂安磺灵对入侵内阿米巴的排胞和后囊发育的抑制作用

• 发表时间: 2002
• 期刊: J Parasitol 88
• 作者: Makioka A;Takeuchi T et al.
• 通讯作者: Takeuchi T et al.

Makioka A, Takeuchi T et al.: "Effect of proteasoiae inhibitors on the growth, encystation and excystation of Entamoeba histolytica and Entamoeba invadens"Parasitol Res. 88・4. 454-459 (2002)

Makioka A、Takeuchi T 等人：“蛋白酶抑制剂对溶组织内阿米巴和侵入内阿米巴的生长、包囊和排泄的影响”Parasitol Res 88·4 (2002)。

Haghighi A, Takeuchi T et al.: "Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geogranphic area"J Clin Microbiol. 40・11. 4081-4090 (2002)

Haghighi A，Takeuchi T 等：“来自有限地理区域的溶组织内阿米巴分离物的显着遗传多态性”J Clin Microbiol 40・11（2002）。