Identifying Regulation of Cell Cycle Gene Networks in E*

识别 E* 中细胞周期基因网络的调控

基本信息

批准号：
7173863
负责人：
UPINDER SINGH
金额：
$ 3.09万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-01 至 2009-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7173863
关键词：
Amebiasis Amebic colitis Biology Bispecific Antibody 2B1 Cell Cycle Cell Cycle Progression Cell Cycle Regulation Cell Nucleus Cell Proliferation Cell division Cell surface Colitis Collaborations Colon Colonic Diseases Condition Cyclins DNA Microarray Chip DNA Microarray format Data Disease Dominant-Negative Mutation Double-Stranded RNA Drug resistance Entamoeba dispar Entamoeba histolytica Enterobacteriaceae Enterocytes Environment Erythrocytes Erythrophagocytosis Eukaryota Eukaryotic Cell Facility Construction Funding Category Family Gene Expression Generations Genes Genetic Genome Genomics Genus Cola Goals Grant Hand Homologous Gene Human In Vitro India Infection Ingestion Institutes Invasive Laboratories Lead Ligand Binding Liver Abscess Medical Surveillance Methodology Methods Microarray Analysis Molecular Numbers Nutrient Parasites Parents Pathogenesis Pathway interactions Pattern Phagocytosis Phase Phenotype Quality Control Regulation Reproducibility Research Sequence Homology Signal Transduction Stimulus Stress Technology Transfer Testing Transcriptional Regulation United States National Institutes of Health Virulence Virulence Factors Work Writing base cdc Genes cohort deprivation genetic analysis in vivo insight interest member monolayer mouse model novel parent grant pathogen prevent research study response species difference tool

项目摘要

DESCRIPTION (provided by applicant): This research will be done primarily at the Bose Institute in Calcutta, India in collaboration with Anuradha Lohia as an extension of NIH grant R01AI 53724. The human pathogen Entamoeba histolytica is responsible for causing amoebic dysentery and liver abscesses. Infection of the human gut is quickly followed by proliferation and multiplication of the parasite, which may then lead to colonic disease. We are interested in identifying molecular factors regulating cell proliferation in this protist parasite, in an effort to devise new strategies to prevent amoebiasis. The goal of the parent proposal is to identify novel amoebic virulence factors by microarray based transcriptional profiling. The FIRCA supplement is written with Prof. Anuradha Lohia who has been studying the regulation of cell cycle progression Entamoeba histolytica. Results from Anuradha's laboratory in India have shown that in E. histolytica genome reduplication may occur without cell division and single nuclei may contain several genome contents during the proliferative phase (Gangopadhyay et al, 1997a; Das and Lohia, 2002). Therefore, unlike most eukaryotes the cell cycle of E. histolytica does not appear to be controlled by known checkpoint surveillance mechanisms. It would be important therefore to identify a global profile of genes that regulate cell cycle progression in E. histolytica. In this proposal we plan to use dsRNA interference (Kaur and Lohia, 2004) to down-regulate cell cycle homologues- Eh Cdks and cyclins, followed by transcriptional profiling using microarrays in order to identify groups of genes controlled by CDKs and cyclins in E. histolytica. This work has important implications for identifying novel aspects of cell cycle control in E. histolytica. Additionally, this project is an ideal collaborative environment in which a main goal is technology transfer (use and analysis of microarray data) to the Lohia lab in India. Arrays as a post-genomic tools are powerful yet highly specialized, thus the "hands-on" use of this application by Lohia's group is an important component of this work.

描述（由申请人提供）：这项研究将主要在印度加尔各答的 Bose 研究所与 Anuradha Lohia 合作进行，作为 NIH 拨款 R01AI 53724 的延伸。人类病原体溶组织内阿米巴负责引起阿米巴痢疾和肝脓肿。人体肠道感染后，寄生虫很快就会增殖和繁殖，从而可能导致结肠疾病。我们有兴趣确定调节这种原生寄生虫细胞增殖的分子因子，以努力制定预防阿米巴病的新策略。母提案的目标是通过基于微阵列的转录分析来识别新的阿米巴毒力因子。 FIRCA 增刊由 Anuradha Lohia 教授撰写，他一直在研究溶组织内阿米巴细胞周期进程的调节。印度 Anuradha 实验室的结果表明，在溶组织内阿米巴中，基因组重复可能在没有细胞分裂的情况下发生，并且在增殖期单个核可能包含多种基因组内容（Gangopadhyay 等，1997a；Das 和 Lohia，2002）。因此，与大多数真核生物不同，溶组织内阿米巴的细胞周期似乎不受已知的检查点监视机制控制。因此，确定调节溶组织内阿米巴细胞周期进程的基因的整体概况非常重要。在本提案中，我们计划使用 dsRNA 干扰（Kaur 和 Lohia，2004）下调细胞周期同源物 - Eh Cdks 和细胞周期蛋白，然后使用微阵列进行转录分析，以确定大肠杆菌中由 CDK 和细胞周期蛋白控制的基因组。溶组织病。这项工作对于识别溶组织内阿米巴细胞周期控制的新方面具有重要意义。此外，该项目是一个理想的协作环境，其主要目标是将技术转让（微阵列数据的使用和分析）到印度的 Lohia 实验室。阵列作为后基因组工具，功能强大但高度专业化，因此 Lohia 团队对该应用程序的“实际”使用是这项工作的重要组成部分。