Analyses of action of a fruit component on the proliferation, invasion and metastasis of cancer cells and on cancerous hyperlipidemia.

分析水果成分对癌细胞增殖、侵袭和转移以及癌性高脂血症的作用。

基本信息

批准号：
11460058
负责人：
YAGASAKI Kazumi
金额：
$ 7.74万
依托单位：
TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Effect of a fruit component, resveratrol, on the proliferation, invasion and metastasis of hepatoma cells, AH1 09A, and on cancerous hyperlipidemia in the hepatoma-bearing rats was studied in vitro and in vivo. Resveratrol significantly inhibited the proliferation and invasion of AH 109A in vitro. This food component was orally given to fasted rats, blood was collected at appropriate time intervals after administration, serum was prepared and subjected to the in vitro proliferation and invasion assays. Serum obtained 1 hr after oral administration most strongly suppressed the invasion but serum obtained 3 hr after oral administration lost the suppressive effect. In contrast, sera failed to suppress the hepatoma proliferation at any time points. Resveratrol-loaded rat sera dose-dependently suppressed the invasion, while they exerted no influence on the hepatoma proliferation even at a high dose of 100 mg/100 g of body weight. Resveratrol and resveratrol-loaded serum canceled the reactive oxygen species (ROS)-induced increase in the invasive capacity by scavenging ROS. The inhibitory activity of resveratrol on the invasion of the hepatoma cells was canceled by addition of prostaglandin (PG) E_2 to experimental media, indicating a possible involvement of PG in the invasion of AH109A. Reveratro dose-dependently decreased the hepatoma-induced hyperlipidemia and serum lipid peroxide concentration in hepatoma-bearing rats.

体外和体内研究了水果成分白藜芦醇对肝癌细胞 AH1 09A 的增殖、侵袭和转移以及对肝癌大鼠癌性高脂血症的影响。白藜芦醇在体外显着抑制AH 109A的增殖和侵袭。将该食物成分口服给禁食的大鼠，给药后每隔适当时间采血，制备血清并进行体外增殖和侵袭测定。口服后1小时获得的血清最强烈地抑制侵袭，但口服后3小时获得的血清失去抑制作用。相反，血清在任何时间点都未能抑制肝癌增殖。负载白藜芦醇的大鼠血清剂量依赖性地抑制侵袭，而即使在100 mg/100 g体重的高剂量下，它们对肝癌增殖也没有影响。白藜芦醇和白藜芦醇负载血清通过清除活性氧 (ROS) 消除活性氧 (ROS) 诱导的侵袭能力增加。在实验培养基中添加前列腺素(PG)E_2可消除白藜芦醇对肝癌细胞侵袭的抑制活性，表明PG可能参与了AH109A的侵袭。 Reveratro 剂量依赖性地降低肝癌大鼠中肝癌诱导的高脂血症和血清脂质过氧化物浓度。