Studies on actions of vegetable components on hyperlipidemia and tumor growth

植物成分对高脂血症和肿瘤生长作用的研究

基本信息

批准号：
08660149
负责人：
YAGASAKI Kazumi
金额：
$ 1.41万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

(1) Inhibition of Cancerous Hypercholesteromia by Cabbage Extract, Its mechanism and Effective Component (s) :Donryu rats subcutaneously implanted with AH109A,a rat ascites hepatoma cell line, were orally given either water (contrl, 1 ml of distillled water /day/100g body weight), cabbage exract solution (CJ,1 ml of 100% cabbage juice/day/100g body weight), or S-methyl-L-cysteine sulfoxide (SMCS,1 ml of 2.5% SMCS aqueous solution/day/100g body weight) solution once a day for 14 days. CJ lowered the serum total cholesterol (T-Ch) level, which was due to the reduction in the very low density + low density lipoprotein (VLDL+LDL) -Ch level, while it enhanced fecal bile acid excretion and cholesterol 7alpha-hydroxylase activity, the rate-limiting enzyme of bile acid synthesis. SMCS.a component of cabbage, could mmic the effect of Cj. These results suggest that cabbage suppresses the hepatoma-induced hypercholesterolemia by up-regulating cholesterol catabolism and that SMCS in cabbage is one … More of the factors suppressing hypercholesterolemia in the hepatoma-bearing rats.(2) Inhibitory Effect of Hepatoma Growth by Concurrent Administration of Cabbage Extract and Lipopolysaccharide :Donryu rats 10 days after implantation of AH109A were divided into 5 groups with equal tumor sizes, and given (1) oral intubation of water (control), (2) oral intubation of 1% cabbage juice, (3) water and intraperitoneal injection of lipopolysaccharide (LPS,10 mug/0.5ml/100g body weight), (4) 1% cabbage juice and LPS,and (5) 100% cabbage juice and LPS,for another 13 days. The concurrent administration of 1% cabbage juice and LPS could signifiantly suppress the growth of the solid hepatoma, while the administration of 100% cabbage juice and LPS in combination, 1% cabbage juice alone, or LPS alone failed to suppress the hepatoma growth.(3) Modes of Actions of Cabbage Juice to Stimulate Tumor Necrosis Factor-alpha Production : Cabbage juice succeeded in stimulating tumor necrosis factor-alpha (TNF-alpha) production in whole splenocytes in culture, whereas it failed to stimulate it in peritoneal macrophages primarily cultured from rat abdominal cavity. However, TNF-alpha production was stimulated by cabbage juice when the latter cells were co-cultured with T lymphocytes from spleen. These results suggest that cabbage juice may indirectly stimulate TNF-alpha production in macrophages through the activation of T cells.(4) Characterization of Effective Component (s) in cabbage Juice That Stimulates Tumor Necroisis Factor-alpha Production :Characterization of effective component (s) in Cabbage juice was conducted using TNF-alpha productivity as an index of anticancer activity in cultured whole splenoctes. Results indicated that effective component (s) had molecular weights less than 10 kDa and stability to heat and acid treatment. Thus, active component (s) may be non-proteinic substance (s) with thermostable and acid-stable properties. Less

(1) 卷心菜提取物抑制癌性胆汁过多症、其机制和有效成分：皮下植入大鼠腹水肝癌细胞系 AH109A 的 Donryu 大鼠，口服水（对照，1ml 蒸馏水/天/ 100克体重），卷心菜提取液（CJ，1毫升100％卷心菜CJ降低了血清总胆固醇（SMCS，1ml 2.5%SMCS水溶液/天/100g体重）溶液，每天一次，持续14天，这是由于极低密度的降低。 + 低密度脂蛋白（VLDL+LDL）-Ch 水平，同时增强粪便胆汁酸排泄和胆固醇 7α-羟化酶活性（卷心菜中胆汁酸合成的限速酶）可以模拟 Cj 的作用。这些结果表明卷心菜通过上调胆固醇分解代谢和抑制肝癌引起的高胆固醇血症。表明卷心菜中的SMCS是抑制肝癌大鼠高胆固醇血症的因素之一。(2)同时给予卷心菜提取物和脂多糖的肝癌生长：将AH109A植入后10天的Donryu大鼠分为肿瘤大小相等的5组，并给予(1)经口插管水(对照)、(2)经口插管1%卷心菜汁，（3）水和脂多糖（LPS，10）腹腔注射杯/0.5ml/100g体重），（4）1%卷心菜汁和LPS，和（5）100%卷心菜汁和LPS，再同时施用1%卷心菜汁和LPS 13天，可以显着抑制。实体肝癌的生长，而100%卷心菜汁和LPS联合给药、单独1%卷心菜汁或单独LPS未能抑制(3) 卷心菜汁刺激肿瘤坏死因子-α 产生的作用模式：卷心菜汁成功刺激了培养物中整个脾细胞的肿瘤坏死因子-α (TNF-α) 产生，但未能刺激它在主要从大鼠腹腔培养的腹膜巨噬细胞中，然而，当卷心菜汁与来自大鼠的淋巴细胞共培养时，TNF-α的产生受到刺激。这些结果表明，卷心菜汁可以通过T细胞的活化间接刺激巨噬细胞中TNF-α的产生。(4)卷心菜汁中刺激肿瘤坏死因子-α产生的有效成分的表征：有效成分的表征使用 TNF-α 生产力作为培养的整个脾脏的抗癌活性指标，对卷心菜汁中的（s）进行了研究，结果表明有效成分具有。分子量小于10kDa并且对热和酸处理的稳定性因此，活性成分可以是具有热稳定性和酸稳定性的非蛋白质物质。