Studies on suppressive mechanisms against soil borne diseases due to the root endophytic fungus, Heteroconium chaetospira.

根部内生真菌 Heterocium chaetospira 抑制土传病害机制的研究。

基本信息

批准号：
11460018
负责人：
HASHIBA Teruyoshi
金额：
$ 8.7万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460018/
关键词：
Heteroconium chaetospira Symbiosis in plants Induction of systemic resistance root endophyte Salicylic acid PR-1a PAL Suppression of symptoms against A. brassicae 黒班病菌 PDF1.2 地上部への抵抗性の誘導宿主植物根への定着エチレンの生成エチレン応答

项目摘要

Root-colonizing fungi were isolated from a total of 663 root segments of Chinese cabbage plants grown in soils collected from wheat, rape, Chinese cabbage, and napier grass fields. Sixteen isolates almost completely suppressed clubroot in sterile soil. Amongst these 16 isolates, two from Heteroconium chaetospira were also effective in nonsterile soil. Chinese cabbage seedlings from seed treated with these two isolates appeared healthy, and inoculation with one isolate promoted growth. Hyphae of the fungus covered the root surface and extensively colonized the inner cortical tissues.The root endophytic fungus, Heteroconium chaetospira isolate OGR-3, was tested for its ability to induce systemic resistance in Chinese cabbage against bacterial leaf spot caused by Pseudomonas syringae pv. macricola and Alternaria leaf spot caused by Alternaria brassicae. Chinese cabbage seedlings inoculated with the isolate of H. chaetospira were incubated in a growth chamber for 32 days. The induced seedlings were challenge inoculated with P. syringae pv. macuricola or A. brassicae. The inoculated Chinese cabbage showed a significant decrease in the lesion number of bacterial leaf spot or Alternaria leaf spot when compared to the non-treated control plants. This indicated that H. chaetospira can induce systemic resistance on foliar by infecting roots.

从总共有663个根部的中国卷心菜植物中分离出根殖民地真菌，这些木材中的中国白菜植物在小麦，强奸，中国卷心菜和纳皮尔草田中种植的土壤中种植。在无菌土壤中，有16种几乎完全抑制了Clubroot。在这16个分离株中，来自杂核的两个分离株在非周围土壤中也有效。用这两种分离株处理的种子中的中国白菜幼苗看起来很健康，接种一次分离株可促进生长。真菌的菌丝覆盖了根部表面并广泛定植了内部皮质组织。根本内生真菌，杂胆芯螺旋体分离株OGR-3，其能力诱导中国卷心菜对细菌叶片的全身性耐药性的能力进行了测试。甲壳虫植物会引起的Macricola和替代叶斑。将与H. Chaetospira分离株接种的中国白菜幼苗在生长室中孵育32天。诱导的幼苗是用丁香假单胞菌PV接种的挑战。 Macuricola或A. brassicae。与未经处理的对照植物相比，接种的中国白菜在细菌叶点或替代叶斑点的病变数量显着减少。这表明H. chaetospira可以通过感染根部引起叶面的全身抗性。