Structural analysis and signal transduction of the host recognition factors expressed in hairpin loop linear DNA plasmid in the plant pathogenic fungus

植物病原真菌发夹环线性DNA质粒表达的宿主识别因子的结构分析和信号转导

• 批准号: 06454058
• 负责人: HASHIBA Teruyoshi
• 金额: $ 4.86万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454058/
• 关键词: hairpin loop linear plasmid; Signal transduction; specific protein; synthetic peptide; single leucine-rich motif; 細胞壁局在性; 変異タンパク質; 植物体への導入; 抵抗性の誘導; 合成ペプチド; Rhizoctonia solani; 菌体内タンパク質; プラスミドコード新規タンパク質; 宿主決定因子; タンパク質の一次構造; 特異的抗体; 菌体内所在

Hairpin loop plasmids were found in the field isolate of Rhizoctonia solani. The open reading frames found in pRS64-1, -2, and -3 (ORF1-1, ORF2-1, and ORF3-1) were 68 amino acid long, respectively. The amino acid sequence encoded by the ORF-1 showed MetThrLeuProLysAlaGlyAlapheThrAsnArgproAspArgArgLeupro-GlyValAsnSerAlaSerGlyAfgserTrpMetLysLeuproCysLysSerGluGlyAsnCysLEUSerMetProTrpGlyIleValLeuArgSerHisSerLeuSerGlyLeuAspArgLeuProLeuGGluProProLysValAla from N-terminal. The amino acid sequence encodid by these ORFs showed no significant homology with known proteins. Extracts from Escherichia coli cells expressing ORF-1 gene a specific new protein of 7 kDa. Anti-pRS64-encoded protein (RS64) antibodies raised against the synthetic peptide (35 and 33 aminoacid residues from ORF-1 N-and C-terminal) cross-reacted with the specific protein found in the mycelia. RS64 protein is located in cell wall of the mycelia. The results indicate that the single leucine-rich motif in RS64 protein involved in protein-protein interactions and transduction. In other words, the specific protein, which may be involved in the host recognition and the hyphal asnastomosis, was expressed by the hairpin loop linear plasmids in R.solani.

发夹环质粒在溶质根神经元的田间分离。在PRS64-1，-2和-3（ORF1-1，ORF2-1和ORF3-1）中发现的开放式读取框分别为68个氨基酸。 ORF-1编码的氨基酸序列显示 MetThrLeuProLysAlaGlyAlapheThrAsnArgproAspArgArgLeupro-GlyValAsnSerAlaSerGlyAfgserTrpMetLysLeuproCysLysSerGluGlyAsnCysLEUSerMetProTrpGlyIleValLeuArgSerHisSerLeuSerGlyLeuAspArgLeuProLeuGGluProProLysValAla from N-terminal.这些ORF包含的氨基酸序列与已知蛋白质没有明显的同源性。来自表达ORF-1基因的大肠杆菌细胞的提取物是7 kDa的特定新蛋白。抗PRS64编码的蛋白（RS64）抗体，抗体与与菌丝体中的特定蛋白质交叉反应的合成肽（35和33个氨基酸残基）。 RS64蛋白位于菌丝体的细胞壁中。结果表明，参与蛋白质 - 蛋白质相互作用和转导的RS64蛋白中的富含亮氨酸基序。换句话说，可能与宿主识别有关的特异性蛋白质和菌丝腺病与R.Solani中的发夹环线性质粒表示。

项目成果

Katsura, K.: "The complete nucleotide sequence and characterization of the linear DNA plasmids pRS64 from the plant pathogenic fungus Rhizoctonia solani" Curr.Genet.(in Press). (1997)

Katsura, K.：“来自植物病原真菌立枯丝核菌的线性 DNA 质粒 pRS64 的完整核苷酸序列和特征”Curr.Genet.（出版中）。

K. Katsura et al.: "The complete nucleotide sequence and characterization of the linear DNA plasmids pRS64 from the plant pathogenic fungus Rhizoctonia solani." Curr. Genet.30. (1997)

K. Katsura 等人：“来自植物病原真菌立枯丝核菌的线性 DNA 质粒 pRS64 的完整核苷酸序列和特征。”

桂 幸次他: "菌類のプラスミドDNA." 日菌報. 38. (1997)

Koji Katsura 等人：“真菌的质粒 DNA。”38。（1997 年）

T. Hashiba et al.: "Rhizoctonia Species : Toxonomy, Molecular Biology, Ecology, Pathology and Disease Control." Kluwer Academic Publishers, 578 (1996)

T. Hashiba 等人：“丝核菌种类：毒理学、分子生物学、生态学、病理学和疾病控制”。

Hashiba, T.: "The Dictionary of Plant Pathology" Yokendo Ltd.1260 (1995)

Hashiba, T.：“植物病理学词典” Yokendo Ltd.1260 (1995)