Creation of High Performance Biocompatible Surface by Surface modification agent which Constructs High Density Tethered Chains

通过构建高密度系链的表面改性剂创建高性能生物相容性表面

基本信息

批准号：
10558139
负责人：
NAGASAKI Yukio
金额：
$ 4.48万
依托单位：
SCIENCE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

We have synthesized seceral types of hydrophilic-hydrophobia block copolymers which forms supramolecular nanostructure in aqueous media due to the self-assembly of the block copolymers. For example, poly(ethylene glycol)/poly(lactide) block copolymer forms spherical core-shell type polymeric micelle in aqueous media, the size of which was 20-40 nm. The shell of the micelle was constructed by tethered PEG chains, whose density was very high in principle because of the thermodynamic balance.The objective of our study was to construct polymer micelle covered surface. If the surface was covered by spherical polymer micelles densely, the density of tethered chains should be much higher that conventional brushed chain surface.We prepared two types of polymer micelle. One of them is poly(2-hydroxyethyl methacrylate)-b-poly(alkylsilylstyrene) (HA) block copolymer micelle. By casting this polymer micelle on the substrate surface, the micelle adsorbed on the surface densely. Platelet adhesion to the modified surface was investigated. The adhesion on the HA-micelle modified surface was mush suppressed than that of not only native substrate but also poly(HEMA) surface. Entropy elastic effect of dense tethered chain may be the reason for this phenomena.Alternately, PEG/PLA block copolymers possessing aldehyde group at PEG chain end and methacryloyl group at PLA chain end was prepared. The block copolymer in aqueous media forms core shell type polymer micelle possessing aldehyde groups on the surface. The methacryloyl group in the core could be polymerized radically to form non dissociative micelle. To a primary-amino containing substrate surface, the aldehyde-micelle was modified covalently via reductive amination reaction. The obtained surface was very dence in polymer micelle and extremely high non-fouling character.These types of highly tethered chain surface was anticipating as new biocompatible surface modification agents.

我们已经合成了隔离类型的亲水性热恐惧症阻滞共聚物，该共聚物由于块共聚物的自组装而在水性培养基中形成超分子纳米结构。例如，聚（乙二醇）/聚（乳酸）嵌段共聚物形成球形核壳型聚合物胶束，其大小为20-40 nm。胶束的壳是由束缚的钉链构造的，由于热力学平衡，其原理的密度非常高。我们研究的目的是构造聚合物胶束覆盖的表面。如果表面被球形聚合物胶束密集地覆盖，则束缚链的密度应比常规拉丝链表面高得多。我们准备了两种类型的聚合物胶束。其中之一是聚（2-羟基乙基甲基丙烯酸酯）-b-poly（烷基甲硅烷基苯乙烯）（HA）块共聚物胶束。通过将此聚合物胶束铸在底物表面上，胶束被密集地吸附在表面上。研究了对修饰表面的血小板粘附。与不仅是天然底物而且是poly（hema）表面相比，HA-MICELLE修饰的表面上的粘附被抑制。密集的束缚链的熵弹性效应可能是这种现象的原因。隔离，在PEG链端具有醛基的PEG/PLA块共聚物，并制备了PLA链端的甲基丙烯酰基组。水性培养基中的块共聚物形成核心壳型聚合物胶束，表面上具有醛基。核心中的甲基丙烯酰基可以从根本上聚合以形成非解离胶束。对于含有底物表面的原代氨基，通过还原性胺化反应共价修饰醛 - 丝甲。所获得的表面在聚合物胶束中非常稳定，并且非常高的非污染特征。这些类型的高度束缚链表面被预期为新的生物相容性表面修饰剂。