Function and regulation of intermediate filament organization during cell prolifenation and cell signalings.

细胞增殖和细胞信号传导过程中中间丝组织的功能和调节。

• 批准号: 10480205
• 负责人: INAGAKI Masaki
• 金额: $ 6.59万
• 依托单位: Aichi Cancer Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10480205/
• 关键词: Phosphorylation; Cleavage furrow; Phosphopeptide antibodies; Rho; Cytokinesis; Intermediate filament (IF); Rho-associated kinase (Rho-kinase); Rho-キナーゼ; ケラチン結合蛋白質; 中間径フィラメント構築制御; 細胞周期; 中間径フィラメント(1F)蛋白質; リン酸化反応; リン酸化ペプチド抗体; Rho

Intermediate filaments (IFs), which form the structural framework of cytoskeleton, have been found to be dramatically reorganized during mitosis. Some protein kinases activated in mitosis are thought to control spatial and temporal IF reorganization through phosphorylation of IF proteins. Rho-associated kinase (Rho-kinase), one of. the putative targets of the small GTPase Rho, does phosphorylate IF proteins, specifically at the cleavage furrow during cytokinesis. This cleavage furrow-specific phosphorylation plays an important role in the local IF breakdown and efficient separation of IF networks, Recent studies on Rho signaling pathways have allowed new models about the molecular mechanism of rearrangements of cytoskeletons including IFs during cytokinesis.

构成细胞骨架结构框架的中间细丝（IFS）已被发现在有丝分裂过程中已大量重组。如果通过IF蛋白的磷酸化进行重新组织，则认为在有丝分裂中激活的一些蛋白激酶可以控制空间和时间。 Rho相关激酶（Rho-kinase），之一。小GTPase Rho的推定靶标，如果蛋白质（特别是在细胞因子过程中的裂解沟中）会磷酸化。这种裂解犁沟特异性磷酸化在IF网络的局部IF和有效分离中起着重要作用，对RHO信号传导途径的最新研究允许有关细胞骨架重排的分子机制的新模型，包括细胞因子期间IF，包括IF。

项目成果

H.Kosako: "Specific Accumulation of Rho-associated kinase at the Cheavage Funow during cytokinesisi Cleauage furrow-spocific phesphory lation of intermediate filament." Oncogene. (in press).

H.Kosako：“在胞质分裂过程中，Rho 相关激酶在 Cheavage Funow 上的特定积累，中间丝的 Cleauage 沟特异性磷酸化。”

Inagaki, N., Nishizawa, M., Minakata, Y., Yamamoto, H., Takeuchi, Y., Miyamoto, E., Kaibuchi, K., and Inagaki, M.: "Activation of Ca^<2+>/calmodulin-dependent protein kinase llwithin post-synaptic dendritic spines of cultured hippocampal neurons."J.Biol.

Inagaki, N.、Nishizawa, M.、Minakata, Y.、Yamamoto, H.、Takeuchi, Y.、Miyamoto, E.、Kaibuchi, K. 和 Inagaki, M.：“Ca^<2 >/ 的激活”

M.Fujita: "Cell cycle-and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexesia Role for cdc2 Kinase" J.Biol.Chem. 273. 17095-17101 (1998)

M.Fujita：“人 MCM 异六聚体复合体的细胞周期和染色质结合状态依赖性磷酸化对 cdc2 激酶的作用”J.Biol.Chem。

Izawa,I., et al.: "Identification of Mrj,a DnaJ/Heat shock protein 40family protein,as a keratin 8/18 filament-regulatory protein."J.Biol.Chem.. 275. 34521-34527 (2000)

Izawa,I., et al.：“Mrj 的鉴定，一种 DnaJ/热休克蛋白 40 家族蛋白，作为角蛋白 8/18 细丝调节蛋白。”J.Biol.Chem.. 275. 34521-34527 (2000)

Yasui, Y., Amano, M., Nagata, K., Inagaki, N., Nakamura, H., Saya, H., Kaibuchi, K., and Inagaki, M.: "Roles of Rho-associated kinase in cytokinesis ; Mutations in Rho-associated kinase phosphorylation sites impair cytokinetic segregation of glial filamen

Yasui, Y.、Amano, M.、Nagata, K.、Inagaki, N.、Nakamura, H.、Saya, H.、Kaibuchi, K. 和 Inagaki, M.：“Rho 相关激酶在胞质分裂中的作用