THREE DIMENSIONAL RECONSTRUCTIONS OF THE CLEAVAGE FURROW

乳沟沟的三维重建

• 批准号: 8362553
• 负责人: JOHN SCHIEL
• 金额: $ 3.19万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362553
• 关键词: Actins; Actomyosin; Anaphase; Cell physiology; Cellular Structures; Cytokinesis; Cytoskeleton; Endosomes; Event; Excision; Funding; Grant; Laboratories; Lipids; Mediating; Mitosis; Modeling; National Center for Research Resources; Organelles; Principal Investigator; Recycling; Regulation; Research; Research Infrastructure; Resources; Role; Source; Staging; Tomogram; United States National Institutes of Health; Vesicle; Work; cost; daughter cell; electron tomography; reconstruction; spatiotemporal; telophase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cytokinesis beginning in early anaphase represents the last stages of mitosis where two daughter cells become separated via an event termed abscission. As the daughter cells progress through cytokinesis they form a cleavage furrow that ingresses by the action of an actomyosin contractile ring. This forms a narrow cytoplasmic bridge that needs to be resolved to produce two separate daughter cells. Additionally, it has been shown that recycling endosomes are required for successful cytokinesis and abscission. Unfortunately, the mechanism and function of endosomes at the cleavage furrow remain unclear. Two hypotheses have been proposed that try to explain the role of endosomes during cytokinesis. The first hypothesis, a luminal filling model, suggests synchronized endosome fusion at the furrow mediates the scission of daughter cells. The second hypothesis, a delivery model, proposes a function for endosomes in the regulation of actin cytoskeleton dynamics and/or lipid composition at the furrow through the delivery of endosomal vesicles. Previous work from our laboratory and others have established a role for recycling endosomes in mediating late stage cytokinesis. Rab11 and FIP3 have been shown to be involved in targeting of recycling endosomes to the cleavage furrow during cytokinesis . To determine the spatiotemporal dynamics of Rab11/FIP3 associated endosomes within the cleavage furrow, we will use electron tomography to three dimensionally reconstruct the cleavage furrow. Comparison of early and late telophase cleavage furrow tomograms will yield a sense of what organelles populate the furrow during telophase. Additionally the use of immunoEM will provide for the identification of organelles populating the cleavage furrow.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 从早期后期开始的细胞因子是有丝分裂的最后阶段，其中两个子细胞通过称为脱落的事件分离。随着子细胞通过细胞因子的发展，它们会形成裂解沟，通过肌动肌球蛋白收缩环的作用将其进出。这形成了一个狭窄的细胞质桥，需要解决以产生两个独立的子细胞。此外，已经表明，成功的细胞因子和脱落需要回收内体。不幸的是，内体的机理和功能尚不清楚。已经提出了两个假设，试图解释内体在细胞因子中的作用。第一个假设是一种腔内填充模型，表明在犁沟时同步的内体融合介导了子细胞的细胞。第二个假设是一个递送模型，提出了通过内体囊泡的递送在犁沟调节肌动蛋白细胞骨架动力学和/或脂质组成的函数。我们实验室和其他人的先前工作已经确立了回收内体中介导晚期细胞因子的作用。 RAB11和FIP3已显示与细胞因子期间的裂解内体靶向裂解。为了确定裂解沟中RAB11/FIP3相关内体的时空动力学，我们将使用电子断层扫描来三维重建裂解沟。比较早期和晚期的末期裂解沟断层图将对末期过程中的细胞器填充沟渠的感觉。另外，使用免疫系统将提供识别散布裂解沟的细胞器。