ANALYSIS OF GENOMIC STRUCTURE OF HEPATITIS G VIRUS (GBV-C/HGV) AND DEVELOPMENT OF SENSITIVE DETECTION SYSTEM OF THE VIRAL GENOME

庚型肝炎病毒(GBV-C/HGV)基因组结构分析及病毒基因组灵敏检测系统的开发

基本信息

批准号：
09470087
负责人：
OKAMOTO Hiroaki
金额：
$ 6.66万
依托单位：
JICHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

During 1995 and 1996, a possible agent for non-A to E hepatitis, designated hepatitis G virus (HGV) or GB virus C (GBV-C), was discovered in the United States. In this research project, the entire nucleotide sequence of Asian HGV strain (GT230 [9390 nucleotides]) was determined and its genomic organization was characterized. The GT230 isolate lacked a clearly identifiable core gene as in the reported isolates. The 3'-untranslated region was highly conserved and utilized as a target for the sensitive detection of HGV RNA of all five genotypes (G1-G5) by the RT-PCR.HGV RNA was detected in 15 (1.2%) of the 1303 healthy blood donors. Of the 2979 women, 32 (1.1%) were positive for HGV RNA.Twenty-six (77%) of 34 babies born to these viremic women were positive for the virus, indicating that HGV is frequently transmitted from mother to infant in the general population. The most critical factor was the HGV viral load in the maternal serum. The genotype G3 was prevalent in healthy individuals and hepatitis patients. Whereas other genotypes of HGV were frequently found in patients with hemophilia who had received imported coagulation factor concentrates in the past.The HGV prevalence in patients with non-B non-C chronic liver disease was comparable with that in patients with type B or type C chronic liver disease, and no difference was observed in demographic features, biochemical factors and histological findings among patients with or without HGV, suggesting that the contribution of HGV infection to liver diseases of unknown etiology would be small. However, certain HGV strain (s) or emerging variant (s) may be pathogenic under special clinical and epidemiological settings. We must keep HGV under suspicion and under study. More evidence is needed before we can reach a final decision about the pathogenesis of HGV.

在1995年和1996年，在美国发现了一种非A肝炎的可能药物，指定为乙型肝炎病毒（HGV）或GB病毒C（GBV-C）。在该研究项目中，确定了亚洲HGV菌株（GT230 [9390核苷酸]）的整个核苷酸序列，并表征了其基因组组织。与报道的分离株中，GT230分离物缺乏可明显识别的核心基因。 3'-非翻译区是高度保守的，并用作通过RT-PCR.HGV RNA对所有五种基因型（G1-G5）敏感检测的靶标，在1303名健康血液供体的15（1.2％）中被检测到。在2979名妇女中，有32名（1.1％）的HGV RNA为阳性。这些病毒性妇女出生的34名婴儿中有十六名（77％）对该病毒呈阳性，这表明HGV经常从母亲传播到普通人群中的婴儿。最关键的因素是母体血清中的HGV病毒载量。基因型G3在健康个体和肝炎患者中普遍存在。而在过去接受过进口凝血因子浓度的血友病患者中经常发现其他HGV的基因型。非B非C慢性肝病患者的HGV患病率与B型或C型C慢性肝病患者中的患者相当，并且在Democraphy Trapity和Intotical Chistigation the Inspient ang not the HING中没有发现HIN的患者的HGV患病率，而HGV患者的HGV患病率与Demography Tription和Notistical Chemogation Chemitigation nof Demoction Hymograpent Chemitigation Hymogation Firving the HING Indervient Intervient Interigation H.对未知病因的肝病会很小。但是，在特殊的临床和流行病学环境下，某些HGV菌株或新兴变体可能是致病性的。我们必须保持HGV的怀疑和研究。在我们可以做出有关HGV发病机理的最终决定之前，需要更多证据。