Body size evolution in Priapulida – ancestrally or secondarily small?

普里普利达的体型进化——先祖小还是继而小？

• 批准号: 454319699
• 负责人: Professor Dr. Andreas Schmidt-Rhaesa
• 金额: --
• 依托单位: Abteilung Wirbellose Tiere
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/454319699?language=en
• 关键词: Body; size; evolution; Priapulida; ancestrally

We are interested in changes of body size during evolution. The taxon Priapulida is ideal to investigate changes of body size, as among the only 21 described species are microscopic (2-3 mm) and macroscopic (several cm, up to 40 cm) species. With the ultimate goal to investigate genetic changes and drivers of evolutionary body size changes, we propose in this project to work out a robust phylogeny and compare morphological changes during ontogeny and between microscopic and macroscopic species. For a phylogeny (work package 1) we want to sequence transcriptomes of six species from the genera Priapulus, Priapulopsis, Halicryptus and Tubiluchus and complement these with data from other working groups on further species and genera (Meiopriapulus, Maccabeus). In three further work packages we will compare different organs (body cavity, musculature, cuticle, excretory organs, gonads) with different techniques (µCT, semithin and histological sectioning, phalloidin staining, tubulin staining). The work packages include comparison through the entire development of Priapulus caudatus, comparison between small (Tubiluchus) and large (Priapulus) species and intrageneric comparison of small (Halicryptus spinulosus) and large (H. higginsi) species.

我们对进化过程中身体尺寸的变化感兴趣，普利阿普利达分类群是研究身体尺寸变化的理想选择，因为在仅有的 21 个描述的物种中，有微观（2-3 毫米）和宏观（几厘米，最大 40 厘米）物种。为了研究遗传变化和进化体型变化的驱动因素，我们建议在这个项目中制定一个稳健的系统发育并比较个体发育过程中以及微观和宏观物种之间的形态变化。在系统发育（工作包 1）中，我们希望对 Priapulus、Priapulopsis、Halicryptus 和 Tubiluchus 属的 6 个物种的转录组进行测序，并用其他工作组关于其他物种和属（Meiopriapulus、Maccabeus）的数据来补充这些数据。将使用不同的技术（μCT、半薄和工作包包括 Priapulus caudatus 整个发育过程的比较、小型 (Tubiluchus) 和大型 (Priapulus) 物种之间的比较以及小型 (Halicryptus spinulosus) 和大型 (H. higginsi) 的属内比较。 ） 物种。