Architecture of the Central Nervous System in Nemathelminthes

线虫中枢神经系统的结构

• 批准号: 5453208
• 负责人: Professor Dr. Andreas Schmidt-Rhaesa
• 金额: --
• 依托单位: Abteilung Wirbellose Tiere
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5453208?language=en
• 关键词: Architecture; Central; Nervous; System; Nemathelminthes

The project wants to provide detailed information on the nervous system of representatives ofthe Nemathelminthes based on immunohistological staining techniques and confocal laser scanning microscopy. The first two phases produced basic information on the information content of single stainings in reference to the entire nervous system as well as methodological tools to work with animals having a cuticle. Data collection has been successful in three of the five taxa included into the project. 26 species of Gastrotricha were investigated, spanning the entire range of gastrotrich diversity. In the third project phase, few gaps (such as representatives of Lepidodasyidae) should be filled and the available data will be phylogenetically analysed. Two species of Priapulida were investigated and two further ones were collected, which will be investigated partly in the remaining time in the second phase of the project and partly in the third phase. Data for two kinorhynch species were obtained, with developmental data and a further species being scheduled for the third phase. Staining of nematomorph larvae was unsuccessful, but now controlled access to material is possible and the staining procedure will be optimized. In the third phase, adults will also be investigated. The collection of Loricifera was unsuccessful, but the collection attempt should be repeated. Within the third phase of the project, the collection and investigation of the species should be finalized. In parallel, joint analyses will be made. The groups working on nervous systems within the SPP are currently working on a glossary. In phase 3, a data matrix will be assembled and analyzed and morphological data are made available for an analysis together with molecular data gained within the SPP.

该项目希望基于免疫组织学染色技术和共焦激光扫描显微镜提供有关线虫代表神经系统的详细信息。前两个阶段产生了有关整个神经系统的单次染色信息内容的基本信息以及用于具有角质层的动物的方法工具。该项目所包含的五个类群中的三个已成功收集数据。对 26 种腹毛虫进行了研究，涵盖了腹毛虫多样性的整个范围。在项目的第三阶段，应填补一些空白（例如 Lepidodasyidae 的代表），并对现有数据进行系统发育分析。对两种 Priapulida 进行了调查，并收集了另外两种，部分将在项目第二阶段的剩余时间内进行调查，部分将在第三阶段进行。获得了两种动吻动物的数据，以及发育数据和计划在第三阶段进行的其他物种的数据。线虫幼虫的染色不成功，但现在可以控制材料的获取，并且染色程序将得到优化。在第三阶段，还将对成年人进行调查。吸血蜂的采集未成功，但应重复采集尝试。在项目的第三阶段，应完成物种的收集和调查。同时，将进行联合分析。 SPP 内的神经系统研究小组目前正在制定术语表。在第 3 阶段，将组装和分析数据矩阵，并将形态学数据与 SPP 内获得的分子数据一起用于分析。