The response of a bony single cell to the quantitive mechanical stress by an atomic force microscope

原子力显微镜观察骨单细胞对定量机械应力的响应

• 批准号: 22890022
• 负责人: SUZUKI Makoto
• 金额: $ 1.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890022/
• 关键词: 骨細胞; 骨芽細胞; 原子間力顕微鏡; カルシウム応答; メカニカルストレス

We examined the form at the tip of cantilever, the quality of the material, and the processing conditions of the cell towards the establishment of a system which performs quantitive mechanical stress load to a single cell using an atomic force microscope. Then, quantity of the stimulus loaded on the cell was examined. When mechanical stress was loaded on the cell by 60nN and the cell was observed with the optical microscope after calcium response analysis, many cells which damaged the cell membrane were seen. Then, the quantity of the stimulus in which it is suitable for calcium response observation, and damage to a cell membrane cannot break out easily was determined. Although measurement data was due to be totaled based on these conditions, AFM equipment is damaged for an earthquake disaster and measurement is impossible. Now, it is preparing towards re-operation of AFM equipment.

我们检查了悬臂尖端的形式，材料的质量以及使用原子力显微镜向单个细胞执行定量机械应力载荷的系统的细胞的处理条件。然后，检查了在细胞上加载的刺激的数量。当钙反应分析后用光学显微镜观察机械应力在细胞上加载到细胞上时，许多细胞会损坏细胞膜。然后，确定适合钙反应观察的刺激的数量，并且对细胞膜的损伤无法轻易爆发。尽管将根据这些条件总计进行测量数据，但AFM设备因地震灾难而损坏，并且不可能进行测量。现在，它正在准备重新操作AFM设备。

项目成果

骨組織形成に関与するCCN2/CTGFのアンカーペプチドアプタマーの設計とその有用性

参与骨组织形成的CCN2/CTGF锚定肽适配体的设计及其用途

• 发表时间: 2010
• 作者: 川木晴美;鈴木誠;藤井俊哉;久保田聡;滝川正春;山本照子
• 通讯作者: 山本照子

Mandibular body lengthening by distraction osteogenesis for correction of skeletal class II problems with an impacted premolar.

通过牵引成骨延长下颌体，以矫正阻生前磨牙的 II 类骨骼问题。

• 发表时间: 2010
• 期刊: J Oral Maxillofac Surg.
• 作者: Takahashi I;Terao F;Suzuki M;Kawamura H;Takano-Yamamoto T.
• 通讯作者: Takano-Yamamoto T.