Developments of transmission-blocking vaccine for tick-borne protozoan diseases

蜱传原虫疾病传播阻断疫苗的研制

• 批准号: 19208026
• 负责人: FUJISAKI Kouzo
• 金额: $ 30.53万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19208026/
• 关键词: マダニ; Babesia原虫; マダニ生物活性分子; TBM; アミノ酸; TOR; オートファジー; ワクチン; TOR情報伝達経路; BD-TBM; TORシグナル伝達機構; S6K; GATA; TBV; TOR経路; リボソーム; 細胞内バイオマス; リジン分解; 多重ビテロジェニン

(1) We could identify for the first time vitellogenins (Vg), vitellogeni-receptor (VgR), and longipain as tick-bioactive molecules (TBM) specifically reacted with sexual and sporogonic stages (S-S satages) of Babesia parasites developing in the vector ticks. (2) Since Vg and VgR could inhibit completely the Babesia transmission in ticks treated with their dsRNA and could suppress effectively the Babesia transmission in animals vaccinated with them, the identification of Babesia molecules reacted with them is now concentrated. (3) However, longipain could promote Babesia development in vector ticks after RNAi, it is considered that its application as candidate molecule for the transmission-blocking vaccine may be difficult under the current research situation lacking of the TBM over0expression systems. (4) During the period of this research, we started to focus on TBMs involved in cellular biomass (CB)control in host cells for Babesia parasites because it was noticed that the development of intra-cellular parasites is fundamentally influenced by the essential nutrients supply under the control of CB. (5) Therefore, the molecular identification and characterization of a series of TBMs involving in transcription, translation, post translational modification, and vesicular transport, autophagy (AT) of intra-cellular proteins are concentrated for the first time. (6) These studies are elucidating the significance of TOR pathways (TORs) as candidate molecules for the transmission-blocking vaccine.

（1）我们可以首次识别卵黄蛋白（VG），Vitellogeni受体（VGR）和Longipain作为tick-Bioactive Molecules（TBM），该分子（TBM）与性和孢子型阶段（S-S-S Satages（S-S Satages）在载体中发生的Babesia parasites）专门反应。 （2）由于VG和VGR可以完全抑制用其dsRNA处理的壁虱中的Babesia传播，并可以有效地抑制接种疫苗的动物中的Babesia传播，因此与它们反应的Babesia分子的鉴定现已浓缩。 （3）但是，Longipain可以在RNAi之后促进Babesia在载体壁虱中的发育，因此在缺乏TBM过度表达系统的当前研究情况下，它作为传输阻滞疫苗的候选分子的应用可能很困难。 （4）在这项研究期间，我们开始专注于宿主细胞中涉及Babesia寄生虫细胞细胞中涉及细胞生物量的TBM，因为人们注意到细胞内寄生虫的发展从根本上受到CB控制下必需的营养供应的影响。 （5）因此，涉及转录，翻译，翻译后修饰和囊泡转运的一系列TBM的分子鉴定和表征，首次浓缩细胞内蛋白的自噬（AT）。 （6）这些研究阐明了TOR途径（TOR）作为传输阻滞疫苗的候选分子的重要性。

项目成果

Molecular interface between Babesia parasites and tick vitellogenesis associated molecules, especially vitellogenin receptor

巴贝虫寄生虫与蜱卵黄发生相关分子，特别是卵黄原受体之间的分子界面

• 发表时间: 2010
• 作者: Yabuta;Y.;藤崎幸蔵
• 通讯作者: 藤崎幸蔵

A cysteine protease is critical for Babesia spp. transmission in Haemaphysalis ticks.

• DOI: 10.1371/journal.ppat.1000062
• 发表时间: 2008-05-16
• 期刊: PLOS PATHOGENS
• 影响因子: 6.7
• 作者: Tsuji, Naotoshi;Miyoshi, Takeharu;Battsetseg, Badger;Matsuo, Tomohide;Xuan, Xuenan;Fujisaki, Kozo
• 通讯作者: Fujisaki, Kozo

創薬開発のマダニ生物活性分子

用于药物发现和开发的蜱生物活性分子

• 发表时间: 2009
• 作者: Uchida K;Shiuchi T;Inada H;Minokoshi Y;Tominaga M.;藤崎幸蔵
• 通讯作者: 藤崎幸蔵

Identification and characterization of Forkhead traqnscription factor from the hard tick, Haemaphysalis longicornis

长角血蜱硬蜱叉头转录因子的鉴定和表征

• 发表时间: 2010
• 作者: Boldbaatar D;白藤(梅宮)梨可;田仲哲也;松岡輝重;藤崎幸蔵
• 通讯作者: 藤崎幸蔵

マダニの吸血消化の分子基盤 : 最近の話題

蜱虫血液喂养和消化的分子基础：最新主题

• 发表时间: 2010
• 作者: Yamada;H.;Nakamura;F.;Watanabe Y.;Murakami;M. and Nogami;T. Reply to conkmenton 'Yalnada H;Nakamura F;Watanabe Y;Murakami M and Nogami T. 2005.;藤崎幸蔵
• 通讯作者: 藤崎幸蔵