Mechanism of molecular basis for "Tailor-made and measurable physical exercise program "and the educational application, evaluated from stress protein (molecular chaperone).

“量身定制、可测量的体育锻炼计划”的分子基础机制和教育应用，从应激蛋白（分子伴侣）进行评估。

基本信息

批准号：
17200039
负责人：
ATOMI Yoriko
金额：
$ 30.95万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

The principle of "tailor-made and measurable physical exercise program' has been studied from dual protein fiber structures of the cytoskeleton and extracellular matrix (ECM), and their molecular chaperone (stress proteins) for main tissues creating exercise, muscles and joints, and their constituting cells. Important results are as follows; 1) The C-terminal located crytallin domain in B-crystallin (B) works as chaperone against tubulin aggregation. 2) As GFP-1 B in beating cultured heart cells shows striation, it must function as chaperone for structural proteins. 3) The N terminal region of OB plays to decrease depolymerization of MAP-microtubule. 4) Disuse muscle atrophy induces the decrease of specific collagen chaperone HSP47 in ER. Similar results were obtained in cultured myoblasts. Possible new gravity-response element in the regulatory domain of HSP47 gene will be identified. 5) The increased temperature of 2 11 of cultured muscle cells facilitates muscle differentiation and increase slow myosin expression. 6) Mild running exercise induces type III collagen, hyaluronic acid synthase in rat knee joint, meaning existence of "good exercise" to contribute to change toward younger cellular environment in our body. 7) Phosphorylated CB/HSP27 and ubiquitinated proteins in human sedentary elderly skeletal muscle increase, suggesting lowered protein degradation. 8) HSP70 was coprecipitated with a key enzyme of glucocorticoid synthesis: StAR in adrenal gland, the peripheral tissue of HPA axis. Stress protein is available for a marker of good and measurable, adaptation connecting both cells and body. 1 earning program of cellular changes by exercise was introduced into compulsory curriculum of physical education and health science for 1st year students the University of Tokyo from 2006. Results of questionnaires by students indicated a contribution of the effectiveness of such a mixed education of knowledge learning and experiment using own body.

从细胞骨架和细胞外基质（ECM）的双蛋白纤维结构及其对产生运动的主要组织、肌肉和关节的分子伴侣（应激蛋白），研究了“量身定制、可测量的体育锻炼计划”的原理。其组成细胞的重要结果如下： 1) B-晶状体蛋白 (B) 中的 C 端晶状体蛋白结构域可作为对抗微管蛋白聚集的伴侣。跳动的培养心脏细胞显示条纹，它必须充当结构蛋白的伴侣。 3) OB 的 N 末端区域可减少 MAP 微管的解聚。 4) 废用性肌肉萎缩会导致 ER 中特定胶原蛋白伴侣 HSP47 的减少。在培养的成肌细胞中获得了结果。将鉴定HSP47基因调节域中可能的新重力响应元件。 5) 2的温度升高。 11 培养的肌肉细胞促进肌肉分化并增加慢肌球蛋白表达。 6) 温和的跑步运动会在大鼠膝关节中诱导III型胶原蛋白、透明质酸合酶，这意味着“良好的运动”的存在有助于改变我们体内细胞环境的年轻化。 7) 久坐的老年人骨骼肌中的磷酸化 CB/HSP27 和泛素化蛋白增加，表明蛋白质降解降低。 8) HSP70与HPA轴周围组织肾上腺中糖皮质激素合成的关键酶StAR共沉淀。应激蛋白可作为连接细胞和身体的良好且可测量的适应标记。从2006年起，东京大学一年级学生的体育和健康科学必修课程中引入了“通过运动改变细胞的1个收益计划”。学生的问卷调查结果表明，这种知识学习和知识学习的混合教育的有效性做出了贡献。用自己的身体做实验。