Interaction between Metallome and Proteome in the Marine Ecosystem

海洋生态系统中金属组和蛋白质组的相互作用

基本信息

批准号：
16204046
负责人：
SOHRIN Yoshiki
金额：
$ 32.2万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

1.Development of chemical speciation and in situ determination of bioactive trace metals(1)Determination of suspended particulate Co, Ni, Cu, Zn, Cd and Pb has been developed.(2)Determination of dissolved and acid-dissolvable Zr, Hf, Nb, Ta and W has been developed.(3)In-situ flow analysis of alkalinity and total carbonate has been developed.2.Development of analytical methods for enzymes and proteins of phytoplankton and bacteria(1)Detection of Fe-S protein PsaC and PsbA(D1) in photosynthetic system has been developed.(2)Anti-ferredoxin and anti-flavodoxin antibodies, which cross-react with ferredoxin and flavodoxin of marine diatoms, respectively, have been developed using synthetic peptides in order to estimate their Fe stress.3.Observation of the variation and relationship of metallome and proteome(1)Different distributions of Fe, Co, Ni, Cu, Zn and Cd in seawater between the Sulu Sea, South China Sea and Pacific Ocean were observed.(2)During the second mesoscale iron enrichment experiments in the western subarctic North Pacific (SEEDS-II), the effects of iron enrichment on the phytoplankton community structure and the speciation of bioactive trace metals were examined.(3)On the basis of analysis of photosynthetic proteins, Fe in seawater has been proved to be a major factor controlling the spring bloom of diatoms in the Oyashio region.(4)Diurnal dynamics of biomass of nano-flagellates and ciliates in Suruga Bay was investigated.(5)The effects of trace metals on the activity of extracellular enzymes of microbes have been proved.4.Study on metal uptake mechanism of phytoplankton in culture(1) The size distribution of Fe in culture media of phytoplankton was investigated. Fe ligands exuded from phytoplankton were analyzed by LC-MS.(2)Membrane proteins revealed in red tide forming phytoplankton Prymnesium parvum under Fe deficiency were analyzed.

1.开发了化学物种形成和原位确定生物活性痕量金属（1）开发了悬浮的颗粒Co，Ni，Cu，Cu，Zn，CD和PB的确定。（2）确定溶解的Zr，HF，NB，NB，NB，NB，NB和W的确定已开发出来。已经开发了浮游植物和细菌的酶和蛋白质（1）在光合系统中检测Fe-S蛋白PSAC和PSBA（D1）。（2）抗毒素和抗氟脱氧蛋白抗体的抗毒素和抗氟脱氧蛋白抗体，这些抗体与果氧蛋白和黄素diat剂量的交叉反应相结合，相应地构成了合成剂，并且已经形成了合成的。 stress.3.Observation of the variation and relationship of metallome and proteome(1)Different distributions of Fe, Co, Ni, Cu, Zn and Cd in seawater between the Sulu Sea, South China Sea and Pacific Ocean were observed.(2)During the second mesoscale iron enrichment experiments in the western subarctic North Pacific (SEEDS-II), the effects of iron enrichment on the phytoplankton community structure and the检查了生物活性痕量金属的形成。（3）基于光合蛋白的分析，已经证明，海水中的铁是控制oyashio地区硅藻弹簧的主要因素。（4）纳米 - 固定物的生物量和ciluiateS的生物量的昼夜动力学在Suruga bay的生物量进行了效果。已经证明了微生物的酶。4。培养物中浮游植物的金属摄取机理（1）研究了浮游植物培养基中Fe的大小分布。通过LC-MS分析了从浮游植物的Fe配体。（2）在FE缺乏下，在红潮形成浮游植物parvum中揭示的膜蛋白。