Dynamics of trace bioelements and their effects on the ecosystem in the marine environment

海洋环境中微量生物元素动态及其对生态系统的影响

基本信息

批准号：
13440166
负责人：
SOHRIN Yoshiki
金额：
$ 9.66万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440166/
关键词：
oceanography analytical chemistry trace metals bioelements phytoplankton chelating ligands carbon dioxide global warming 分析学

项目摘要

1. An analytical method for the determination of trace metals in seawater has been developed on the basis of chelating adsorbent column extraction-ICP mass spectrometry. The distributions of Fe, Co, Ni, Cu, Zn, Cd, and Pb in the North Pacific have been observed. The concentration of acid-dissolvable Fe is higher than that of dissolved Fe, showing the dominance, of labile particulate Fe in seawater. Although dissolved Fe and Zn in summer surface water are depleted in the subarctic North Pacific, they are abundant in the subtropical North Pacific.2. We have found that there was an east-west gradient in the photosynthetic, competence of phytoplankton in the subarctic North Pacific in the summer of 1999 and that the difference was probably caused by Fe levels in seawater. A dramatic floristic shift was found by pigment signatures.3. We have participated a mesoscale Fe enrichment in the western subarctic North Pacific (SEEDS2001). The photochemical quantum efficiency of algal photosystem suggested that the phytoplankton community was suffered from Fe stress and the Fe enrichment improved the physiological condition. There was some 5 nM of labile particulate Fe in the field. It had been supplied by aeolian dust, but it was not readily available for phytoplankton. The decrease in dissolved Co, Ni, Cu, Zn, and Cd occurred during the phytoplankton bloom.4. A sensitive analytical method has been developed using chelating ligand exchange-adsorptive cathodic stripping voltammetry, and Fe ligands derived from eukaryotic phytoplankton have been detected.5. We have developed a technique to separate and purify RNA from phytoplankton under Fe stress. The composition change in membrane proteins of Fe-stressed phytoplankton has been observed.6. Incubation experiments of phytoplankton have shown that the growth of phytoplankton is controlled by the species and concentrations of co-existing organic ligands.

1。基于螯合吸附剂柱提取-ICP质谱的分析方法是开发了海水中痕量金属的分析方法。已经观察到北太平洋中Fe，Co，Co，Ni，Cu，Zn，Cd和Pb的分布。可溶解酸的Fe的浓度高于溶解的Fe的浓度，显示了海水不稳定的颗粒Fe的优势。尽管夏季地表水中溶解的Fe和Zn在北太平洋亚北太平洋中耗尽，但在亚热带北太平洋2中却很丰富。2。我们发现，1999年夏天的北极亚北太平洋浮游植物的光合作用，浮游植物的能力中有一个东西方梯度，这种差异可能是由于海水中的铁水平引起的。颜料特征发现3。我们参加了北太平洋西部亚亚葡萄酒（Seeds2001）的中尺度Fe富集。藻类光系统的光化学量子效率表明，浮游植物群落受到FE压力的折磨，而Fe富集改善了生理状况。该场中有大约5 nm不稳定的颗粒Fe。它是由Aeolian Dust提供的，但不容易用于浮游植物。溶解的CO，Ni，Cu，Zn和CD的减少发生在浮游植物Bloom期间。4。已经使用螯合配体交换 - 吸收阴极剥离伏安法制定了一种敏感的分析方法，并检测到了来自真核浮游植物的Fe配体。5。我们已经开发了一种技术，可以将RNA与FE应力下的浮游植物分离和纯化。已经观察到了Fe胁迫浮游植物的膜蛋白的组成变化。6。浮游植物的孵育实验表明，浮游植物的生长受共存有机配体的物种和浓度控制。