Mechanisms of the development of neuronal cell society in the central nervous system

中枢神经系统神经细胞群的发育机制

• 批准号: 16200027
• 负责人: NAKAJIMA Kazunori
• 金额: $ 25.63万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16200027/
• 关键词: cerebral cortex; neuronal migration; subventricular zone; marginal zone; ventricular zone; gene expression; multipolar migration; development; 神経細胞; 細胞移動; 子宮内胎児脳電気穿孔法; 樹状突起; 中間帯; 電気穿孔法; 層形成; 皮質板; 大脳皮質発生; ジーンチップ

In the developing cerebral cortex, most neurons that are born near the ventricle accumulate in the subventricular zone (SVZ) as multipolar migrating neurons and are then transformed to locomoting neurons prior to entering the cortical plate (CP). When the locomoting cells reach the layer beneath the marginal zone (MZ), we observed that they undergo various dramatic morphological changes, in addition to the termination of migration. For example, they begin to develop apical primitive dendrites and accumulate beneath the MZ, suggesting that they probably start to form new intercellular relationships with adjacent cells and thereby develop an appropriate layer structure. Furthermore, locomoting neurons guided by radial glial fibers in the CP ultimately undergo somal translocation once their leading processes reach the pial surface ("terminal translocation"). To understand the mechanisms that regulate these events, we took several approaches in this study. Firstly, we screened for genes that are specifically expressed in cells in each zone of the embryonic cortical wall: ventricular zone (VZ), SVZ, or cells beneath the MZ. Consequently, we identified approximately 4, 000 genes that are preferentially expressed in the VZ, among which 659 genes are secretory or transmembrane molecules. As for the SVZ and the cells beneath the MZ, we found about 20 genes and 40 genes expressed preferentially in each zone, respectively. We have analyzed the biological functions of some of these genes in this study. To clarify the regulatory mechanisms of gene expression profiles of these molecules in a migratory stage-dependent manner would be important in the future study.

在发育中的脑皮质中，大多数出生的神经元在心室附近出生的脑室下区（SVZ）作为多极迁移神经元积聚，然后在进入皮质板（CP）之前转化为机车神经元。当运动细胞到达边际区域（MZ）以下的层时，我们观察到它们还会发生各种戏剧性的形态变化，除了迁移的终止。例如，他们开始开发顶端原始树突并在MZ下积聚，这表明它们可能开始与相邻细胞形成新的细胞间关系，从而发展出适当的层结构。此外，一旦其领先过程到达PIAL表面（“末端易位”），由CP中的径向神经胶质纤维引导的运动神经元最终会经历som易位。为了了解调节这些事件的机制，我们在这项研究中采用了几种方法。首先，我们筛选了在胚胎皮质壁的每个区域中特异性表达的基因：心室区（VZ），SVZ或MZ下方的细胞。因此，我们确定了在Vz中优先表达的大约4,000个基因，其中659个基因是分泌或跨膜分子。至于MZ下方的SVZ和细胞，我们发现在每个区域中优先表达了大约20个基因和40个基因。我们在这项研究中分析了其中一些基因的生物学功能。为了阐明这些分子以迁移阶段依赖性方式的基因表达谱的调节机制将在未来的研究中很重要。

项目成果

Cell-autonomous roles of ARX in cell proliferation and neuronal migration during corticogenesis

• DOI: 10.1523/jneurosci.1067-08.2008
• 发表时间: 2008-05-28
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Friocourt, Gaeelle;Kanatani, Shigeaki;Parnavelas, John G.
• 通讯作者: Parnavelas, John G.

The cortical subventricular zone-specific molecule Svetl is part of the nuclear RNA coded by the putative Netrin receptor gene Unc5d and is expressed in multipolar migrating cells

皮质室下区特异性分子 Svetl 是由假定的 Netrin 受体基因 Unc5d 编码的核 RNA 的一部分，在多极迁移细胞中表达

• 发表时间: 2008
• 期刊: MoL CelL Neurosci 38
• 作者: Ma J;et. al.;Shinji Sasaki
• 通讯作者: Shinji Sasaki

Analysis of the mechanism of Reelin-induced tyrosine phosphorylation of Dabl

Reelin诱导Dabl酪氨酸磷酸化的机制分析

• 发表时间: 2006
• 作者: Komatsubara;T.;Ken-ichiro
• 通讯作者: Ken-ichiro

In silico analysis of cell sorting that recapitulates the pattern of cellular arrangement in a reproducible manner

细胞分选的计算机分析以可重复的方式概括细胞排列的模式

• 发表时间: 2006
• 作者: Nakamura;Ko.;Fujiyama;F.;Furuta;T.;Kaneko;T;Takuya Maeda
• 通讯作者: Takuya Maeda

PCM1 is recruited to the centrosome by the cooperative action of DISCI and BBS4 and is a candidate for psychiatric illness

PCM1 在 DISCI 和 BBS4 的协同作用下被招募到中心体，是精神疾病的候选者

• 发表时间: 2008
• 期刊: Arch.Gen.Psychiatry (In press)
• 作者: Li J-L;Xiong K;Pang Y-W;Dong Y;Kaneko T;Mizuno N;Atsushi Kamiya
• 通讯作者: Atsushi Kamiya