Genome analysis of bovine major histocompatibility complex region and development of cattle with resistance to disease

牛主要组织相容性复合体区域基因组分析及牛抗病能力的培养

基本信息

批准号：
14206035
负责人：
AIDA Yoko
金额：
$ 33.95万
依托单位：
RIKEN
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14206035/
关键词：
bovine MHC (BoLA) region BoLA-DRB3 BoLA-DQA mastitis BLV-induced lymphoma BLV epitope diseases susceptibility PCR-sequence based typing BoLA-DQA1及び-DQA2 多型性 PCR-Sequence Based Typing BoLA-DOA及び-DQB TAPトランスポーター BoLAクラスI遺伝子 BoLA-

项目摘要

Genomic organization of bovine major histocompatibility complex (BoLA) : The draft sequence of entire bovine genome has been announced by Human Genome Sequencing Center, and the information has been available in NCBI genome project database (http://www.ncbi.nlm.nih.gov/). Here, we analyzed the comparative map of human MHC region on chromosome 6 (HSP6p) and BoLA region on chromosome 23 (BTA23), using the draft sequence of BoLA and complete sequence of human MHC region. Although the existence of two class II subregions in cattle gene map was known before (cf.class IIa and IIb), and is also confirmed in our comparative gene map based on the whole genome draft sequencing, some of the newly constructed gene correspondences show differences from the previous linkage map.Relationship between BoLA class IIa region and development of mastitis and bovine leukemia virus (BLV)-induced lymphoma : BoLA class IIa region revealed to associate in various diseases and economic trait. The class IIa region contains BoLA-DRA, -DRB, -DQA and -DQB genes and shows a high degree of polymorphism. Recently, we developed new methods for sequence based typing (SBT) which allow identification of alleles of BoLA-DQA1 gene and BoLA-DRB3 gene of large numbers of animals with relative case, and showed that the DRB3^*0701-DQA1^*0101 and DRB3^*14012-DQA1^*10012 might protect against development of BLV-induced lymphoma. We now progress to identify BLV epitope which related to both alleles. Next, we performed the typing of DQA1 alleles of 188 cattle with mastitis and 95 normal Holstein cattle and calculated each allele frequency in two groups. The DQA1^*1201 were apparently low frequency in mastitis group as compared with a normal group (p=0.006663), suggesting that this allele might be positively related to resistance to mastitis. Collectively, the breeding by the marker selection using BoLA class II haplotype might probably contribute to the improvement of disease resistance traits in domestic cattle.

牛主要组织相容性复合物（BOLA）的基因组组织：人类基因组测序中心已经宣布了整个牛基因组的草稿序列，并且该信息已在NCBI基因组项目数据库（http://wwww.ncbi.ncbi.nlm.nlm.nih.gov/）中获得。在这里，我们使用BOLA的草稿序列和人类MHC区域的完整序列分析了第6染色体上的人类MHC区域（HSP6P）和BOLA区域上人类MHC区域的比较图。尽管之前已经知道了牛基因图中两个II类子区域的存在（cf.class IIA和IIB），并且在基于整个基因组测序的比较基因图中也得到了证实，但一些新构建的基因对应关系与先前的链接图显示了与先前的链接IIA和BOLA CLEVICALIGHAIG。 Bola IIA级地区透露了各种疾病和经济特征的同事。 IIA类区域包含Bola -Dra，-DRB，-DQA和-DQB基因，并显示出高度的多态性。最近，我们开发了用于基于序列的键入（SBT）的新方法，这些方法允许鉴定大量具有相对情况的动物的Bola-DQA1基因和Bola-Drb3基因的等位基因，并表明DRB3^*0701-DQA1^*0101和DRB3^*14012-DQA1^*14012-DQA1^*1001^*1001可能会抗衡。我们现在进步以识别与两个等位基因相关的BLV表位。接下来，我们进行了188个患有乳腺炎和95个正常荷斯坦牛的188牛的DQA1等位基因，并以两组计算了每个等位基因频率。与正常组相比，乳腺炎组的DQA1^*1201显然是低频率的（P = 0.006663），这表明该等位基因可能与对乳腺炎的耐药性呈正相关。总体而言，使用Bola II类单倍型的标记选择繁殖可能有助于改善家养牛的抗病性状。