Clinical application of diagnosis method of bovine major histocompatibility complex genes associated with resistanceand susceptibility to bovine leukemia virus-induced lymphomain East Asia

牛主要组织相容性复合体基因与牛白血病病毒东亚淋巴瘤耐药和易感相关的诊断方法的临床应用

• 批准号: 22405040
• 负责人: AIDA Yoko
• 金额: $ 11.56万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22405040/
• 关键词: BoLA クラスIIDRB3; PCR-Sequence based typing(SBT)法; BLV,BLV nested PCR; フィリピン; ホルスタイン; ブラーマン; Native cattle; BoLAクラスIIDRB3; PCR-SBT法; 新規・既知アリル; 東南アジア; 固有品種; 交雑種; 多型性; BoLAクラスII DRB3・DQA1; BLV nested PCR; 中国山東省

We previously demonstrated that bovine leukocyte antigens (BoLA)-DRB3 alleles associated with resistance or susceptibility to development of bovine leukemia virus (BLV)-induced B lymphoma and analyzed distribution of these alleles in Japan and South-America. However, until now, none of the South-east Asian breed had been characterized for BoLA-DRB3 genes by PCR-sequence based typing (SBT). Hence, we here sequenced exon 2 of the BoLA class II DRB3 gene from 981 cattle including Holstein, Sahiwal, Brahman, Philippine Native and its cross breed, corrected from five islands of Philippine using modified SBT method which is suitable for Philippine cattle. In addition, we detected BLV by a new BLV-CoCoMo-qPCR. We identified the 75 previously reported alleles and 6 novel alleles. Interestingly, the number found in each breed is extent high level, indicating that that Asian breeds reveal extent high level of polymorphism for BoLA-DRB3 distributions. Our result also showed BLV-infected cattle with the susceptibility-associated allele was significantly lower than that in Japan.

我们先前证明了牛白细胞抗原（Bola）-DRB3等位基因与抗抗性或易感性有关牛白血病病毒（BLV）诱导的B淋巴瘤的易感性以及日本和南美洲的这些等位基因的分布。但是，到目前为止，没有通过基于PCR序列的键入（SBT）来表征Bola-DRB3基因的东南亚品种。因此，我们在这里对Bola II类DRB3基因的外显子2进行了测序，其中包括荷斯坦，Sahiwal，Brahman，Brahman，Philippine及其Cross Broud，使用适合菲律宾牛的改良SBT方法，从菲律宾的五个岛屿上校正了。此外，我们通过新的BLV-Cocomo-QPCR检测到BLV。我们确定了75个先前报道的等位基因和6个新等位基因。有趣的是，在每个品种中发现的数量是高水平的，表明亚洲品种揭示了Bola-DRB3分布的高度多态性。我们的结果还表明，受易感性相关的等位基因的受BLV感染的牛明显低于日本。

项目成果

A new method for typing bovine major histocompatibility complex class II DRB3 alleles by combining two established PCR sequence-based techniques

• DOI: 10.1111/j.1399-0039.2011.01708.x
• 发表时间: 2011-09-01
• 期刊: TISSUE ANTIGENS
• 作者: Takeshima, S. -N.;Matsumoto, Y.;Aida, Y.
• 通讯作者: Aida, Y.

HIV-1 Vprによる細胞周期G2期停止に関与する宿主因子のハイスループットスクリーニング法の確立

HIV-1 Vpr诱导细胞周期G2期阻滞相关宿主因子高通量筛选方法的建立

• 发表时间: 2012
• 作者: 村上知行;間陽子
• 通讯作者: 間陽子

Diversity of BOLA class II DRB3 and DQA1 alleles among different herds of Japanese Black and Holstein cattle in Japan

日本不同群体的日本黑牛和荷斯坦牛中 BOLA II 类 DRB3 和 DQA1 等位基因的多样性

• 发表时间: 2010
• 作者: 宮坂卓;竹嶋伸之輔;松本有生;小林直彦;松橋珠子;石橋和樹;泉對博;間陽子
• 通讯作者: 間陽子

Characterization of Bovine Leukemia Virus Tax protein and its mutants

牛白血病病毒Tax蛋白及其突变体的表征

• 发表时间: 2010
• 作者: Arainga Ramirez Mariluz Anamelva;武田英里;間陽子
• 通讯作者: 間陽子

Identification of bovine leukocyte antigen (BoLA) class II haprotypes that are regulated bovine leukemia virus (BLV) proviral load in Japanese Black cattle

日本黑牛中受牛白血病病毒 (BLV) 前病毒载量调节的牛白细胞抗原 (BoLA) II 类单体型的鉴定

• 发表时间: 2012
• 作者: Aida Y.;Miyasaka T.;Jimba M.;Matsumoto Y.;Kobayashi N.;Matsuhashi T.;Sentsui H.,Takeshima S-n.
• 通讯作者: Sentsui H.,Takeshima S-n.