The non-invasive diagnosis of the rejection after heart and lung transplantation

心肺移植排斥反应的无创诊断

基本信息

批准号：
06671323
负责人：
OUMI Mikio
金额：
$ 1.22万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

We visualized in vitro and in vivo myocardial beta-adrenergic receptors in rat iso-and allograft hearts (LV working model) with autoradiography, using an imasing plate and [3H]-CGP 12177. We also examined localization and alteration in lesioned graft hearts and found a close relationship with acute rejection. Receptor localization in the native hearts was homogeneous but patchy in the heterotopically transplanted allograft hearts, especially in the inner layr of the left ventricular wall, right ventricular wall and inter-ventricular septum. Mixed areas of high-and low-density caused this patchy hetero-geneity. High-density areas correlated with up-regulation and low-density areas with degeneration, collapse and necrosis of cardiac myofibers with infiltrating mononuclear cells, i. e., the existence of acute rejection. Both in vitro and in vivo images were characteristically similar. Acute rejection, using the in vivo technique, was identified as low-density areas in the right and left ventricu alls as early as 4 days following transplantation. This method which incorporated [3H]-CGP 12177 in vivo was useful to detect acute rejection in the early and late phase. Clinically, positron emission tomography with [11C]-CGP 12177 may be useful for early detection of acute rejection noninvasively and may have several important advantages over other radioisotopes and endomyocardial biopsy.

我们使用成像板和 [3H]-CGP 12177，通过放射自显影观察了大鼠同种和同种异体移植心脏（LV 工作模型）的体外和体内心肌 β-肾上腺素能受体。我们还检查了受损移植心脏的定位和变化，以及发现与急性排斥反应有密切关系。原生心脏中的受体定位是均匀的，但异位移植的同种异体移植心脏中的受体定位是斑片状的，特别是在左心室壁、右心室壁和室间隔的内层。高密度和低密度的混合区域造成了这种不均匀的异质性。高密度区域与上调相关，低密度区域与心肌纤维变性、塌陷和坏死以及浸润的单核细胞相关，即。 e.存在急性排斥反应。体外和体内图像在特征上相似。早在移植后 4 天，使用体内技术就将急性排斥反应识别为右心室和左心室中的低密度区域。这种在体内掺入[3H]-CGP 12177的方法可用于检测早期和晚期的急性排斥反应。临床上，[11C]-CGP 12177 正电子发射断层扫描可能有助于非侵入性早期检测急性排斥反应，并且可能比其他放射性同位素和心内膜心肌活检具有几个重要优势。