Observation of wet biological specimens by imaging x-ray microscope with the use of zone plates

使用波带板通过成像 X 射线显微镜观察湿生物标本

• 批准号: 04680277
• 负责人: KIHARA Hiroshi
• 金额: $ 1.34万
• 依托单位: Kansai Medical University (1993)Jichi Medical School, School of Nursing (1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680277/
• 关键词: imaging x-ray microscope; zone plate; synchrotron radiation; UVSOR; X線顕微鏡

We have mounted imaging x-ray microscope with two zone plates at UVSOR(Okazaki, Japan), and applied it to observe various bio-specimens dry or wet. With a zone plate as a test specimen, we observed zones as fine as 64 nm, which was very close to theoretical resolution limit (55nm).As dry specimens, we observed specimens such as diatom, myofibril, clamidomonas, of which pictures are shown in the main volume of the present activity report. As wet specimen, we only observed an image of myofibril or its fragment, as the limitation of usable beam time.we realized that we still have several probelems to be solved in future, mainly concerning the condenser zone plate ; specimens are not illuminated uniformly, probably due to the mismatching of optical parameters of condenser zone plate to the micro zone plate, or due to the deformation of the consenser zone plate from the designed values.

我们已经在UVSOR（日本冈崎）上安装了带有两个区域板的X射线显微镜，并将其应用于观察各种生物特异性的干燥或潮湿。以区域板作为测试标本，我们观察到了64 nm的区域，它非常接近理论分辨率极限（55nm）。作为干样本，我们观察到标本，例如硅藻，肌膜，肌纤维，clamidomonas，显示了哪些图片，其中显示了哪些图片显示为其中的图片。在本活动报告的主要卷中。作为湿标本，我们只观察到肌原纤维或其碎片的图像，因为可用的光束时间的限制。我们意识到，将来我们仍然有几个概率可以解决，主要是关于冷凝器区域板的。标本并非均匀照亮，这可能是由于冷凝器区域板的光学参数不匹配到微区板，或者是由于设计值与共有区域板的变形所致。

项目成果

N.Watanabe et al.: "Observation of Wet Bio-Specimen by X-Ray Imaging Microscope with ZonePlate at UVSOR" Jpn.J.Appl.Phys.31. L1571-L1573 (1992)

N.Watanabe 等人：“通过 X 射线成像显微镜在 UVSOR 下使用 ZonePlate 观察湿生物样本”Jpn.J.Appl.Phys.31。

N.Watanabe et al.: "Soft X-Ray Microscope with Sub-Optical Resolution at UVSOR" UVSOR activity report. 1992. 137-138 (1993)

N.Watanabe 等人：“UVSOR 具有亚光学分辨率的软 X 射线显微镜”UVSOR 活动报告。

N.Watanabe et al.: "X‐Ray Microscopy III" Soft X‐Ray Microscopy with Zone Plates at UVSOR, 491(147-150) (1992)

N. Watanabe 等人：“X 射线显微镜 III”UVSOR 波带板软 X 射线显微镜，491(147-150) (1992)

N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates II" UVSOR activity report. 1991. 72-73 (1992)

N.Watanabe 等人：“带有波带板 II 的软 X 射线显微镜”UVSOR 活动报告。

N.Watanabe et al.: "Soft X-Ray Microscope with Zone Plates at UVSOR" UVSOR activity report. 1993. 163-164 (1994)

N.Watanabe 等人：“在 UVSOR 处配备波带板的软 X 射线显微镜”UVSOR 活动报告。