Development and its medical/biological application of a projection X-ray microscope using synchrotron radiation.

使用同步辐射的投影 X 射线显微镜的开发及其医学/生物学应用。

基本信息

批准号：
09309001
负责人：
SHINOHARA Kunio
金额：
$ 20.74万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

Soft X-ray projection microscopy using synchrotron radiation as a light source has been developed. The system consists of a Fresnel zone plate with an outermost zone width of 80nm, a pinhole (0.5-20μmφ), specimens on a specimen holder, and a back-illuminated CCD camera. In some experiments, a pre-pinhole (10-100μmφ) was placed in the upper stream of the zone plate to give coherent illumination to it. The alignment of the pinhole was achieved with the aid of piezo-driven stages with ten-nanometer precision. Monochromatic soft X-rays of the wavelengths of 1.5nm, 2.4nm and 3.6nm were obtained from the beamline BL-12A and BL-11A at the Photon Factory, Tsukuba, Japan. The highest spatial resolution of 0.2-0.3μm was attained with the combination of a 10μmφ prepinhole and a 1μmφ pinhole, judging from the resolution of line spacing on a test pattern consisting of lines and spaces with defined widths. With this system, we obtained images of EM-grids, dried mammalian cells, and dried human chromosomes. Granular structure in mammalian cells and fine structure in chromosomes were distinguished. In parallel with the image acquisition, computational studies were conducted 1) to evaluate optical properties of the combination of a zone plate and a pinhole, and 2) to develop the method for the improvement of the quality of images recovered from the experimentally obtained images distorted by the Fresnel diffraction. Further improvements of the system in 1) the improvement of the resolution, 2) the development for imaging biological specimens in wet environment and 3) the improvement of the quality of images by a computer processing, and its medical/biological application remained to be studied.

已经开发了使用同步加速器辐射作为光源的软X射线投影显微镜。该系统由最外面宽度为80nm，针孔（0.5-20μmφ），标本支架上的标本和后刷的CCD摄像头组成。在某些实验中，将前针（10-100μmφ）放置在区域板的上流流中，以使其具有相干的照明。针孔的对齐是在压电驱动的阶段以十纳米计精度实现的。从日本Tsukuba的Photon Factory的Beamine BL-12A和BL-11A获得了1.5nm，2.4nm和3.6nm波长的单色软X射线。最高的空间分辨率为0.2-0.3μm，与10μmφ前孔和1μmφ针孔的组合相连，从线间距的分辨率来看，这是由线间距的分辨率判断，该测试模式由带有定义宽度的线和空间组成。使用该系统，我们获得了EM网格，干燥的哺乳动物细胞和干染色体的图像。区分了哺乳动物细胞中的颗粒结构和染色体中的精细结构。与图像采集并行，进行了计算研究1）评估区域板和针孔组合的光学性质，以及2）开发方法，以提高从淡线衍射扭曲的实验获得的图像中恢复的图像质量。系统在1）分辨率的改进中进一步改进，2）在潮湿环境中成像生物学标本的开发以及3）通过计算机处理来提高图像质量，其医疗/生物应用程序仍待研究。