Quantitation of oxygen radaical production of splanchnic organs in animals with various types of shock

不同类型休克动物内脏器官氧自由基产生的定量

基本信息

批准号：
04670942
负责人：
OGAWA Ryo
金额：
$ 1.28万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Three models of organ injury induced by shock (hemorrhagic, splanchnic ischemia and lung injury) were made on adult rats. The methods were reported previously. New techniques were emplyed for trapping of radicals using PBN as a trapper. The details were reprted previously. Radaicals trapped into PBN were analyzed quantitatively using NSR spectrometry.Spin aducts production in liver was increased to 270% after 2 hour of hemorrhagic shock. A small and tempoary elevation of spin aducts prodeuction in bowels was observed immediately after release of splanchinic artery occlusion, followed by reduction to control levels. Spin aducts production increased markedly after 30 minutes after reperfusion and show a peak at 90 minutes after in splanchnic ischemia shock model. On lung injury model radical production elevated linearly after injection of xanthine-xanthine oxidase solution into trachea. Lipid peroxidation increased pararelly to production of oxygen free radaicals in organ of animals subjected to 3 types of injury.Oxygen radical scavengers such as allopurinol and superoxide dismutase were applied on animals with shock. Alopurinol did not show any effective insults, Superoxide dismutase showed a suppressive effect of oxygen radical production in splanchnic ischemia shock and lung injury models.In rats which have no white blood corpuscle by pre-treatment of nitrogen mastard spinaducts production of bowel remained in a low state, suggesting a possible role of these blood cells in produciton of tissue injuries.

由成年大鼠引起的冲击引起的三种器官损伤（出血性，鼻子缺血和肺损伤）。这些方法先前报道了。将新技术充满了使用，用于使用PBN作为捕捞者捕获自由基。详细信息先前已重复。使用NSR光谱法定量分析被困在PBN中的径径。在出血性休克2小时后，肝脏中的Spin Aducts产生增加到270％。释放单张动脉闭塞后立即观察到肠道产量的旋转性产物产生的小而温短的升高，然后降低至对照水平。再灌注后30分钟后，旋转量产生的产生显着增加，并在Splanchnic缺血模型后90分钟显示峰值。在肺损伤模型上，自由基产生在将黄嘌呤 - 黄嘌呤氧化酶溶液注入气管后线性升高。脂质的过氧化增加了遭受3种损伤的动物器官中无氧根本的产生。氧气自由基清除剂（例如别嘌醇和超氧化物歧化酶）被震惊地应用于动物上。丙铂未显示出任何有效的损伤，超氧化物歧化酶在脱骨缺血性缺血和肺损伤模型中表现出氧激活产生的抑制作用。在没有白细胞肌肉的大鼠中，通过预先治疗氮肥大的细胞激素，在低状态下将肠子释放出来，表明在低状态下，表明这些血液的作用表明这些血液的作用可能是这些血液的作用，这些作用可能是这些血液的生产细胞的生产量为生产的生产。