The role of sulfate-reducing bacteria on insolubilization of heavy metal ions at waste disposal sites

硫酸盐还原菌对垃圾处理场重金属离子不溶化的作用

• 批准号: 04650495
• 负责人: TAKAMIZAWA Kazuhiro
• 金额: $ 1.41万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04650495/
• 关键词: sulfate-reducing bacteria; waste disposal site; methane-producing bacteria; 不溶化; 金属硫化物

Distribution of sulfate-reducing bacteria (SRB) at two sea-based solid waste disposal sites and seven landfill sites were conducted from 1993 to 1995, resulting that SRB widely distributed in the sites. SRBs were accumulated and insolubilization experiments using several heavy metal ions were performed. Insolubilization rate of hexavalent chromium was very low of 16%, neverthless, Cd, Cu, Fe, and Zn ions were precipitated within 48 hr reaction with hydrogen sulfide produced by SRBs. In case of Cd ion, produced precipitate was determined as cadmium sulfide from SEM and EDX analysis. The role of SRBs for insolubilization of heavy metal ions in waste disposal sites turned evident. Among SRBs, Desulfotomaculum sp. was isolated. Methane-producing bacteria (MPB) were also widely distributed in sites and MPB number counted from boring-pits samples were larger than those of from leachates. If samples contained 20mg/l or more of sulfuric ion concentration, SRBs number were greater than MPB number. Two kinds of MPB were isolated. One was Methanosarcina sp., which utilized both methanol and acetic acid and the other was thermophilic Methanomicrobium sp. The difference between MPB and other bacteria was confirmed by fluorescence microscopy using 420nm of exciting wavelength, but it was difficult to differ MPB from SRBs by fluorescence microscopy.

1993年至1995年，对2个海基固体废物处置场和7个垃圾填埋场进行了硫酸盐还原菌（SRB）的分布调查，硫酸盐还原菌在场内广泛分布。积累SRB并进行使用几种重金属离子的不溶化实验。六价铬的不溶化率很低，仅为16%，但与SRBs产生的硫化氢反应48小时内，Cd、Cu、Fe和Zn离子就沉淀出来。在Cd离子的情况下，通过SEM和EDX分析确定产生的沉淀物为硫化镉。 SRB 在废物处理场所不溶解重金属离子方面的作用变得明显。在 SRB 中，Desulfotomaculum sp。被孤立了。产甲烷菌（MPB）在场地中也广泛分布，钻孔样品中MPB的数量多于渗滤液中的MPB数量。如果样品含有20mg/l或更高的硫酸离子浓度，SRB数大于MPB数。分离出两种MPB。一种是甲烷八叠球菌 (Methanosarcina sp.)，它同时利用甲醇和乙酸，另一种是嗜热的甲烷微生物 (Methanomicrobium sp.)。使用420nm激发波长的荧光显微镜证实了MPB与其他细菌之间的差异，但通过荧光显微镜很难区分MPB与SRB。

项目成果

Kazuhiro Takamizawa: "Distribution of sulfate-reducing bocteria at a sea-bared solid waste disposal site and their role on insolubilization of heary metal ions in leachate" 廃棄物学会論文誌. 5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原细菌在裸露的固体废物处理场的分布及其对渗滤液中重金属离子不溶性的作用”日本废物管理协会杂志 5. 89-97 (1994)。

Kazuhiro Takamizawa: "Distribution of sulfate-reducing bacteria at a sea-based solid waste site and their role on insolubilization of heavy metal ions in leachate." J.Japan Soc.Waste Management Experts.5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原菌在海基固体废物场的分布及其对渗滤液中重金属离子不溶性的作用。”

Kazuhiro Takamizawa: "Distribution of sulfute-reducing bacteria at a sea-based solid waste disposal site and their role on insolubilization of heavy metal ions in leachate" 廃棄物学会論文誌. 5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原菌在海基固体废物处理场的分布及其对渗滤液中重金属离子不溶性的作用”日本废物管理学会杂志 5. 89-97 (1994)。