The role of sulfate-reducing bacteria on insolubilization of heavy metal ions at waste disposal sites

硫酸盐还原菌对垃圾处理场重金属离子不溶化的作用

• 批准号: 04650495
• 负责人: TAKAMIZAWA Kazuhiro
• 金额: $ 1.41万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04650495/
• 关键词: sulfate-reducing bacteria; waste disposal site; methane-producing bacteria; 不溶化; 金属硫化物

Distribution of sulfate-reducing bacteria (SRB) at two sea-based solid waste disposal sites and seven landfill sites were conducted from 1993 to 1995, resulting that SRB widely distributed in the sites. SRBs were accumulated and insolubilization experiments using several heavy metal ions were performed. Insolubilization rate of hexavalent chromium was very low of 16%, neverthless, Cd, Cu, Fe, and Zn ions were precipitated within 48 hr reaction with hydrogen sulfide produced by SRBs. In case of Cd ion, produced precipitate was determined as cadmium sulfide from SEM and EDX analysis. The role of SRBs for insolubilization of heavy metal ions in waste disposal sites turned evident. Among SRBs, Desulfotomaculum sp. was isolated. Methane-producing bacteria (MPB) were also widely distributed in sites and MPB number counted from boring-pits samples were larger than those of from leachates. If samples contained 20mg/l or more of sulfuric ion concentration, SRBs number were greater than MPB number. Two kinds of MPB were isolated. One was Methanosarcina sp., which utilized both methanol and acetic acid and the other was thermophilic Methanomicrobium sp. The difference between MPB and other bacteria was confirmed by fluorescence microscopy using 420nm of exciting wavelength, but it was difficult to differ MPB from SRBs by fluorescence microscopy.

从1993年至1995年进行了两个基于海的固体废物处理地点和七个垃圾填埋场，硫酸盐还原细菌（SRB）的分布，从而导致SRB广泛分布在该地点。积累了SRB，并使用几种重金属离子进行了无关实验。六价铬的不溶液速率非常低16％，毫无疑问，CD，Cu，Fe和Zn离子在48小时内与SRBS产生的硫化氢的48小时反应。在CD离子的情况下，通过SEM和EDX分析确定产生的沉淀为硫化物。 SRB在废物处理地点不溶解重金属离子的作用变化了。在SRB中，Deulfotomaculum sp。被隔离了。产生甲烷的细菌（MPB）也被广泛分布在位点，从钻孔样品中计数的MPB数量大于渗滤液中的样品。如果样品含有20mg/L或更多的硫酸离子浓度，则SRBS数大于MPB数。分离了两种MPB。一种是甲烷菌Sp。，它同时使用甲醇和乙酸，另一个是嗜热甲烷菌Sp。使用420nm的激动人心的波长通过荧光显微镜证实了MPB和其他细菌之间的差异，但是通过荧光显微镜很难与SRBS不同。

项目成果

Kazuhiro Takamizawa: "Distribution of sulfate-reducing bocteria at a sea-bared solid waste disposal site and their role on insolubilization of heary metal ions in leachate" 廃棄物学会論文誌. 5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原细菌在裸露的固体废物处理场的分布及其对渗滤液中重金属离子不溶性的作用”日本废物管理协会杂志 5. 89-97 (1994)。

Kazuhiro Takamizawa: "Distribution of sulfate-reducing bacteria at a sea-based solid waste site and their role on insolubilization of heavy metal ions in leachate." J.Japan Soc.Waste Management Experts.5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原菌在海基固体废物场的分布及其对渗滤液中重金属离子不溶性的作用。”

Kazuhiro Takamizawa: "Distribution of sulfute-reducing bacteria at a sea-based solid waste disposal site and their role on insolubilization of heavy metal ions in leachate" 廃棄物学会論文誌. 5. 89-97 (1994)

Kazuhiro Takamizawa：“硫酸盐还原菌在海基固体废物处理场的分布及其对渗滤液中重金属离子不溶性的作用”日本废物管理学会杂志 5. 89-97 (1994)。