The effect of tumor necrosis factor-alpha on hypothalamopituitary -ovarian axis

肿瘤坏死因子-α对下丘脑垂体-卵巢轴的影响

• 批准号: 03807107
• 负责人: KOIKE Koji
• 金额: $ 1.15万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807107/
• 关键词: tumor necrosis factor-alpha; anterior pituitary; arachidonate; Hypothalamus; Paracrine; 視床下部; ド-パミン; LHーRH; TNFーα; インタ-ロイキン6; エストロジェン

There is an accumulating evidence of a close linkage between neuroendocrine and immune system. Studies are designed to investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on hypothalamo-pituitary-ovarian axis. First, we demonstrated that TNF-alpha stimulated interleukin-6 (IL-6) production by hypothalamic cells. Further we have shown that IL-6 stimulated GnRH release from hypothalamic cessl(1), suggesting that cytokines such as IL-6 and TNF-alpha are involved in the feedback mechanism of estrus cycle. We also demonstrated that TNF-alpha stimulated IL-6 production by anterior pituitary cells and our results also suggested that folliculo-stellate (FS ) cell is one of the candidates of IL-6-producing cells(2). In addition, we observed that TNF-alpha stimulated the release of arachidonate from anterior pituitary cells and arachidonate metabolites such as 5-lipoxygenase products are involved in the process of prolactin release(3).

越来越多的证据表明神经内分泌和免疫系统之间存在密切联系。研究旨在调查肿瘤坏死因子-α (TNF-α) 对下丘脑-垂体-卵巢轴的影响。首先，我们证明 TNF-α 刺激下丘脑细胞产生白细胞介素 6 (IL-6)。此外，我们还发现IL-6刺激下丘脑进程释放GnRH(1)，表明IL-6和TNF-α等细胞因子参与发情周期的反馈机制。我们还证明 TNF-α 刺激垂体前叶细胞产生 IL-6，我们的结果也表明滤泡星状 (FS) 细胞是 IL-6 产生细胞的候选细胞之一 (2)。此外，我们观察到 TNF-α 刺激垂体前叶细胞释放花生四烯酸，并且花生四烯酸代谢物（例如 5-脂氧合酶产物）参与催乳素释放过程 (3)。

项目成果

Yamaguchi M.: "Interleukin-6 stimulates gonadotropin-releasing hormone secretion from rat hypothalamic cells" Horm.Res.55. 199-203 (1992)

Yamaguchi M.：“Interleukin-6 刺激大鼠下丘脑细胞分泌促性腺激素释放激素”Horm.Res.55。

Yamaguchi M.: "The interferon family stimulates the secretions of prolactin and interleukin-6 by the pituitary bland in vitro." J.Endocrinol.Invest.14. 457-461 (1991)

Yamaguchi M.：“干扰素家族在体外刺激垂体分泌催乳素和白介素 6。”

Masumoto N.: "Pituitary follicuro-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pituitary adenylate cyclase-activating peptide),showing increased adenosine 3'5'-monophosphate and interleukin-6 secietion and cell proliferation."

Masumoto N.：“垂体滤泡星状细胞系 (TtT/GF) 对新型促垂体肽（垂体腺苷酸环化酶激活肽）有反应，显示腺苷 35-单磷酸和白细胞介素 6 部分和细胞增殖增加。

Masahide Ohmichi: "Immunohistochemical evidence that rat FSH cells contain β II-subspecies of protein kinase C" Endocrinol Japon. 39. 609-613 (1992)

Masahide Ohmichi：“大鼠 FSH 细胞含有蛋白激酶 C β II 亚种的免疫组织化学证据”Endocrinol Japon 39. 609-613 (1992)。

Koike K.et al.: "Tumor necrosis factor-alpha increases release of arachidonate and prolactin from anterior pituitary cells" Endocrinology. 128. 2791-2798 (1991)

Koike K.等人：“肿瘤坏死因子-α 增加垂体前叶细胞释放花生四烯酸和催乳素”内分泌学。