Evaluation of a biomarker-based approach for exposure assessment of furan in food

基于生物标志物的食品中呋喃暴露评估方法的评价

• 批准号: 437639884
• 负责人: Professorin Dr. Angela Mally
• 金额: --
• 依托单位: Lehrstuhl für Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/437639884?language=en
• 关键词: Evaluation; biomarker; based; approach; exposure

Furan occurs in a variety of food items that undergo heat treatment. Due to the high volatility of furan and limited understanding of the fate of furan during home cooking, assessment of human exposure based on food consumption data and furan content in food is unreliable. Considering the low margin between current exposure estimates and furan doses that cause adverse health effects in laboratory animals, there is an urgent need for improved exposure assessment as a basis for risk assessment.Taking into account the contribution of possible endogenous formation of furan, the overall aim of the project is to test the validity of a biomarker-based approach to assess exposure to furan via food. Administration of isotopically labelled [3,4-13C]-Furan to rats across a wide dose range that includes doses relevant to human exposure will allow us to precisely establish the correlation between external dose and concentration of furan metabolites in urine over time and to discriminate against endogenous formation and furan intake via feed. These experiments will provide critical information as to which metabolites (isolated or in combination) may be suitable to assess exposure to furan under realistic exposure scenarios. As proof-of-concept, potential biomarkers of furan exposure will be quantitatively analysed in urine of human volunteers following consumption of food with low vs. high furan content to estimate exposure.

Furan发生在接受热处理的各种食品中。由于Furan的高波动性和对家庭烹饪过程中Furan的命运有限的了解，因此基于食物消耗数据和食物中Furan含量的人类暴露是不可靠的。考虑到当前的暴露估计和呋喃剂量之间的较低边缘在实验室动物中造成不利的健康影响，迫切需要改善暴露评估作为风险评估的基础。考虑到可能的内源性呋喃形成的贡献，该项目的整体目的是测试基于生物标志物通过食物进行生物标志物评估呋喃的有效性的方法。在宽剂量范围内给予大鼠的同位素标记[3,4-13C] - 富兰，这将使我们能够精确地建立尿液中尿液代谢物在尿液中的外部剂量和浓度随着时间的流逝，并随着时间的流逝而与内源性形成和通过进料进行歧视。这些实验将提供有关哪些代谢产物（分离或组合）可能适合评估在现实暴露情况下暴露于Furan的关键信息。作为概念验证，在消费较低含量与高呋喃含量以估计暴露的食物之后，将在人类志愿者的尿液中进行定量分析狂暴暴露的潜在生物标志物。