Evaluation of a biomarker-based approach for exposure assessment of furan in food

基于生物标志物的食品中呋喃暴露评估方法的评价

• 批准号: 437639884
• 负责人: Professorin Dr. Angela Mally
• 金额: --
• 依托单位: Lehrstuhl für Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/437639884?language=en
• 关键词: Evaluation; biomarker; based; approach; exposure

Furan occurs in a variety of food items that undergo heat treatment. Due to the high volatility of furan and limited understanding of the fate of furan during home cooking, assessment of human exposure based on food consumption data and furan content in food is unreliable. Considering the low margin between current exposure estimates and furan doses that cause adverse health effects in laboratory animals, there is an urgent need for improved exposure assessment as a basis for risk assessment.Taking into account the contribution of possible endogenous formation of furan, the overall aim of the project is to test the validity of a biomarker-based approach to assess exposure to furan via food. Administration of isotopically labelled [3,4-13C]-Furan to rats across a wide dose range that includes doses relevant to human exposure will allow us to precisely establish the correlation between external dose and concentration of furan metabolites in urine over time and to discriminate against endogenous formation and furan intake via feed. These experiments will provide critical information as to which metabolites (isolated or in combination) may be suitable to assess exposure to furan under realistic exposure scenarios. As proof-of-concept, potential biomarkers of furan exposure will be quantitatively analysed in urine of human volunteers following consumption of food with low vs. high furan content to estimate exposure.

呋喃存在于各种经过热处理的食品中。由于呋喃的高挥发性以及对家庭烹饪过程中呋喃去向的了解有限，根据食品消费数据和食品中呋喃含量评估人类暴露情况是不可靠的。考虑到目前的暴露估计值与对实验动物造成不良健康影响的呋喃剂量之间的差距较小，迫切需要改进暴露评估作为风险评估的基础。考虑到可能的内源性形成呋喃的贡献，总体而言该项目的目的是测试基于生物标记的方法的有效性，以评估通过食物接触呋喃的情况。对大鼠施用同位素标记的[3,4-13C]-呋喃，其剂量范围很广，包括与人体暴露相关的剂量，这将使我们能够精确地建立外部剂量与尿液中呋喃代谢物浓度随时间变化的相关性，并区分防止内源性形成和通过饲料摄入呋喃。这些实验将提供关于哪些代谢物（分离的或组合的）可能适合评估现实暴露场景下呋喃暴露的关键信息。作为概念验证，将对人类志愿者食用低呋喃含量与高呋喃含量的食物后的尿液中的呋喃暴露的潜在生物标志物进行定量分析，以估计暴露情况。