Anticardiolipin antibody and the development of arterial and venous thrombosis.

抗心磷脂抗体与动脉和静脉血栓形成的发展。

• 批准号: 06454249
• 负责人: KOIKE Takao
• 金额: $ 4.54万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454249/
• 关键词: Anticardiolipin antibodies; beta2-glycoprotein I; Antiphospholipid syndrome; Thrombosis; Human monoclonal aCL; Oxidized LDL; 習慣流産

The appearance of anticardiolipin antibodies (aCL) in patients with systemic lupus erythematosus (SLE) and other collagen diseases is associated with variety of clinical features, including arterial and venous thromboembolic manifestation, recurrent fetal loss and thrombocytopenia. These symptoms called antipholipid syndrome (APS).We investigated the specificity of aCL from patients with APS and from its animal model and obtained following results.1) aCL recognized an altered structure of beta2-glycoprotein I (beta2-GPI) .2) The epitope on beta2-GPI is expressed by a conformational change occurring when beta2-GPI interacts with an oxygen-substituted solid-phase surface.3) Human monoclonal aCL-producing cell lines were established from APS patients' lymphocytes.4) Oxidized lipoproteins, especially oxidized LDL,are sequentially targeted by beta2-GPI and aCL.

系统性红斑狼疮 (SLE) 和其他胶原性疾病患者中抗心磷脂抗体 (aCL) 的出现与多种临床特征相关，包括动脉和静脉血栓栓塞表现、反复流产和血小板减少。这些症状称为抗磷脂综合征 (APS)。我们研究了 APS 患者及其动物模型的 aCL 特异性，并获得了以下结果。1) aCL 识别出 β2-糖蛋白 I (beta2-GPI) 结构的改变。2) beta2-GPI 上的表位通过当 beta2-GPI 与氧取代的固相表面相互作用时发生的构象变化来表达。3) 从 APS 建立人单克隆 aCL 生成细胞系4）氧化脂蛋白，尤其是氧化LDL，依次为β2-GPI和aCL的靶向目标。

项目成果

Tsutsumi, A., K.Ichikawa, E.Matsuura, M.Mukai, A.Fujisaku, S.Kobayashi, T.Koike: "Antibodies to beta2-glycoprotein I and clinical manifestaton in patients with systemic lupus erythematousus." Arthritis Rheum. (in press).

Tsutsumi, A.、K.Ichikawa、E.Matsuura、M.Mukai、A.Fujisaku、S.Kobayashi、T.Koike：“系统性红斑狼疮患者的 β2-糖蛋白 I 抗体和临床表现。”

Matsuura.E., Y.Igarashi. T.Yasuda. D.A.Tnplen. T Koike: "Anticardiolipin antibodies recognize β_2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface." J Exp.Med.179. 457-462 (1994)

Matsuura.E.、Y.Igarashi.D.A.Tnplen.T Koike：“抗心磷脂抗体可识别通过与氧修饰的固相表面相互作用而改变的 β_2-糖蛋白 I 结构。” (1994)

E.Matsuura,M.Igarashi,Y.Igarashi,T.Katahira,H.Nagae,K.Ichikawa,D.A.Triplett,T.Koike: "Moleccular studies on phospholipid-binding sites and crypic epitopes appearing on β 2-glycoprotein I structure recognized by anticardiolipin antibodies." Lupus. (in pres

E. Matsuura、M. Igarashi、Y. Igarashi、T. Katahira、H. Nagae、K. Ichikawa、D. A. Triplett、T. Koike：“对 β 2-糖蛋白 I 结构上出现的磷脂结合位点和隐藏表位的分子研究被抗心磷脂抗体识别。”狼疮。（预

K.Ichikawa,Munther A.Khamashta,T.Koike,E.Matsuura,Graham R.V.Hughes: "β 2-glycoprotein I reactivity of monoclonal anticardiolipin antibodies from patients with the antiphospholipid syndrome" Arthritis & Rheumatism. 37. 1453-1461 (1994)

K. Ichikawa、Munther A. Khamashta、T. Koike、E. Matsuura、Graham R. V. Hughes：“抗磷脂综合征患者单克隆抗心磷脂抗体的 β2-糖蛋白 I 反应性”关节炎与风湿病 37。1453-1461（1994 年） ）

T.Koike: "Anticardiolipin antibodies and beta2-glycoprotein I." Clin Immunol Immunopathol. 72. 187-192 (1994)

T.Koike：“抗心磷脂抗体和 β2-糖蛋白 I。”