Pathogenesis of Pulmonary Emphysema

肺气肿的发病机制

• 批准号: 05670539
• 负责人: NAGAI Atsushi
• 金额: $ 1.34万
• 依托单位: Toky Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670539/
• 关键词: Emphysema; Lung injury; Oxidant; Elastase; Niacin; Nicotinamide; DNA damage; Apoptosis; アポートシス; ニコチン; 好中球; DNA; 喫煙; 電子顕微鏡

Neutrophil1. Effects of nicotine in the tabacco smoke on neutrophil survival were investigated. Neutrophil survival prolonged with nicotine in dose related fashion. A transmission electron microscopy and electrophoresis of the exracted DNA revealed that nicotine inhibits neutrophil apoptosis.2. Filtrability of neutrophils exposed to tabacco smoke was investigated. Neutrophils exposed to the smoke showed ultrastructural abnormalities and a decreased filtrability. The decrease in filtrability was due to exposure of oxidants in the gas components of the smoke. Nicotine prevented the decrease in concentration dependent fashion and the effect of nicotine was due to antioxidant effects.Lung injury induced by oxidant1. Lung injury induced by oxidant was inhibited by niacin or nicotinamide. These agents prevented a decrease in NAD which was induced by oxidant exposure.2. Cultured bronchial epithelial cells were damaged by H202 exposure in concentration- and time-dependent fashion. The epithelial damage induced by H202 was inhibited by an addition of niacin, nicotinamide, theophilline, or DBcAMP.Niacin inhibited a decrease in intracellular ATP which was brought about by H202 exposure.

中性粒细胞1.研究了烟草烟雾中尼古丁对中性粒细胞存活的影响。尼古丁以剂量相关的方式延长中性粒细胞的存活时间。提取DNA的透射电镜和电泳结果表明尼古丁具有抑制中性粒细胞凋亡的作用。 2．研究了暴露于烟草烟雾的中性粒细胞的过滤性。暴露于烟雾的中性粒细胞表现出超微结构异常和过滤性下降。过滤性下降是由于烟雾气体成分中暴露了氧化剂。尼古丁阻止了浓度依赖性方式的下降，尼古丁的作用是由于抗氧化作用。氧化剂引起的肺损伤。烟酸或烟酰胺可抑制氧化剂引起的肺损伤。这些药物阻止了由氧化剂暴露引起的NAD的减少。2．培养的支气管上皮细胞因暴露于H2O2而受到浓度和时间依赖性的损害。通过添加烟酸、烟酰胺、茶碱或DBcAMP来抑制H 2 O 2 诱导的上皮损伤。烟酸抑制由H 2 O 2 暴露引起的细胞内ATP的减少。

项目成果

Kazutetsu Aoshiba: "Nicotine prevents a reduction in neutrophil filtrability induced by cigarette smoke exposure" Am Rev Respir Dis. (in press).

Kazutetsu Aoshiba：“尼古丁可防止因接触香烟烟雾而引起的中性粒细胞过滤性降低”Am Rev Respir Dis。

K Aoshiba et al.: "Nicotine prevents a reduction in neutrophil filtrability induced by cigarette smoke exposure" Am J Respir Crit Care Med. 150. 1101-1107 (1994)

K Aoshiba 等人：“尼古丁可防止因接触香烟烟雾而引起的中性粒细胞滤过性降低”Am J Respir Crit Care Med。

A Nagai, et al.: "Effects of nicotinamide and niacin on bleomycin-induced acute injury. and subsequent fibrosis in hamster lungs" Exp Lung Res. 20. 263-281 (1994)

A Nagai 等人：“烟酰胺和烟酸对博来霉素诱导的急性损伤和仓鼠肺随后纤维化的影响”Exp Lung Res。

Kazutetsu Aoshiba: "Transient neutropenia after intravenous injection of vindesine in patients with lung cancer" Eur J Clin Pharmacol.47. 25-32 (1994)

Kazutetsu Aoshiba：“肺癌患者静脉注射长春地辛后出现暂时性中性粒细胞减少症”Eur J Clin Pharmacol.47。

Kazutetsu Aoshiba: "Nicotine prevents a reduction in neutrophil filtrability induced by cigarette smoke exposure" American Journal of Respiratory Critical Care Medicine. 150. 1101-1107 (1994)

Kazutetsu Aoshiba：“尼古丁可防止因接触香烟烟雾而引起的中性粒细胞过滤性降低”《美国呼吸重症监护医学杂志》。