Secretion and gene expression of ovarian hormone in the chicken

鸡卵巢激素的分泌及基因表达

• 批准号: 05660326
• 负责人: TOMOGANE Hiroshi
• 金额: $ 1.47万
• 依托单位: Nippon Veterinary and Animal Science Niversity (1994)Nagoya University (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660326/
• 关键词: P450_<17>alpha_<hydroxylase>; P450aromatase; mRNA; sexual differentiation; ovary; testis; estradiol; arginine vasotocin; ニワトリ; シクロオキシゲナーゼ; ノーザンハイブリダイゼーション; プロスタグランジン; 卵巣; 卵胞膜内細胞; 卵胞膜外細胞; 顆粒膜細胞; テストステロン; エストラジオール; P450アロマターゼ; 遺伝子発現

Study(1). Changes in messenger ribonucleic acid (mRNA) levels of P450_<17>alpha and P450arom of the ovary and testis in the chicken embryos. Since P450aromatase (P450arom) is a key enzyme converting androgen to estrogen, gene expression of P450arom during an early development of chick embryos seems important. In this study mRNA levels of P450_<17>alpha and P450arom of the ovary and testis were determined during the period between day 10 of incubation and day of hatching and the change were correlated with those in gonadal estradiol levels. P450_<17>alpha mRNA levels were higher in the left ovary than in the testis between day 10-14 of incubation but thereafter there was no difference between the left ovary and testis. P450arom mRNA levels wre always higher in the left ovary than in the testis and these changes were paralleled to these of estradiol. The results indicate that higher production of the ovarian estradiol in earlier stage in reflected by mRNA levelds of P450arom of the ovary … More . Study (2). Localization of P450arom mRNA in the gonad in relation to sexual defferentiation in the chicken. In situ hybridization was performed in the gonad of developing chick embryos using P450arom riboprobe. P450arom mRNA was not detected until day 6 of incubation but was observed after day 6.5 in the genetic female gonad, whereas no mRNA was observed in the genetic male gonad. Study (3). Gene expression of arginine vasotocin in the ovary of the laying hen. Using reverse trascription-polymerase chain reaction (RT-PCR) method, mRNA levels of arginine vasotocin were determined in the conpartmentalized cells of the ovarian follicles. During follicular maturation the mRNA levels of the granulosa decreased, but there was no change in the theca interna. Theca externa showed a significant reduction at the second largest follicle but increased at the largest. These results indicate that gene expression of arginine vasotocin in ovarian cells may by regulated differntially during folliclular growth in the chicken. Less

研究（1）。 P450_ <17> alpha和p450arom的卵巢和睾丸的P450_ <17>的信使色带计算器（mRNA）水平的变化。由于P450Aromatase（P450arom）是一种关键酶，将雄激素转化为雌激素，因此在鸡肉胚胎的早期发育过程中，P450arom的基因表达似乎很重要。在这项研究中，在孵化和孵化日的第10天之间，确定了卵巢和睾丸的P450_ <17> alpha和p450arom的mRNA水平，并且变化与性腺雌二醇水平的那些相关。 P450_ <17>左卵巢中的αmRNA水平高于孵化的第10-14天之间的睾丸，但此后左卵巢和睾丸之间没有差异。在左卵巢中，P450arom mRNA水平比睾丸中的P450arom mRNA水平始终高，并且这些变化与雌二醇的这些变化相似。结果表明，在卵巢的mRNA水平反映的卵巢雌二醇的产生中，卵巢雌二醇的产量更高。研究（2）。 P450arom mRNA在性腺中与鸡肉中的性防御有关。使用P450arom rubopobe在发育的雏鸡胚胎的性腺中进行原位杂交。直到孵育的第6天才检测到P450AROM mRNA，但在第6.5天的遗传性雌性性腺中观察到，而在遗传性男性腺中未观察到mRNA。研究（3）。精氨酸血管毒素在躺产的卵巢中的基因表达。使用反向扭转 - 聚合酶链反应（RT-PCR）方法，在卵巢卵泡的综合细胞中测定了精氨酸血管素的mRNA水平。在卵泡成熟期间，颗粒的mRNA水平降低，但国际没有变化。 theca外部显示出第二大叶叶的显着降低，但最大的叶子增加。这些结果表明，精氨酸血管素在卵巢细胞中的基因表达可以通过在鸡卵泡生长过程中受到差异性调节。较少的

项目成果

Tomogane,H.: "A factor(s) from a rat trophublast cell line inhibits prolaction secretion in vivo and in vitro." Biology of Reproduction. 48. 325-332 (1993)

Tomogane,H.：“来自大鼠滋养层细胞系的因子在体内和体外抑制泌乳分泌。”

Shimada/Kiyoshi: "Sanran seigyo no bunshi kiko" Gakujutsu geppo. Insatutyu. (1995)

岛田/清：《三乱世界的文士纪子》学术月炮。

Uchiyama,M.,Saito,N.and Shimada,K.: "Pituitary and plasma arginic vasotocin levels in the lamprey,Lamperta japonica." Comparative Biochemistry & Physiology. 107A. 23-26 (1994)

Uchiyama, M.、Saito, N. 和 Shimada, K.：“七鳃鳗 (Lamperta japonica) 中的垂体和血浆精氨酸催产素水平。”

Sechman, A.: "Tissue-specific expression of calbindin-D_<28k> gene during outogency of the chicken" J.Expt.Zool.269-5. 450-457 (1994)

Sechman, A.：“鸡外显子过程中钙结合蛋白-D_<28k> 基因的组织特异性表达”J.Expt.Zool.269-5。

Kansaku/Nobuo: "Chabo no shuso ni tomonau kasuitai horumon idenshi hatsugen" Toukai tikusan gakkaiho. 4-1. 38-41 (1994)

Kansaku/Nobuo：“Chabo no shuso ni tomonau kasuitai horumon idenshi hatsugen”Toukai tikusan gakkaiho。