Studies on the Regulatory Genes of Gonadal Differentiation from the Viewpoint of Utilization in Animal Resource Science.

从动物资源科学利用的角度研究性腺分化的调控基因。

• 批准号: 06304021
• 负责人: TACHI Chikashi
• 金额: $ 9.73万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06304021/
• 关键词: SRY; c-kit; P450Aromatase; Sertoli cells; Sex determination; apoptosis; ovary; transgenic animals; cyclin; Sry; WAP; fos; P450 Aromatase; 黄体

Representative results obtained during the tenure of the grant are as follows. 1) Coplete genomic SRY (sex determining region on Y chromosome) DNA of Shiba goat (Capra hircus var Shiba) spanning 4.9 kb was cloned and sequenced. 2) Transformed cell lines were established by transfecting TM-4 cells derived from mouse Sertoli cells, with human and mouse SRY DNA.Curiously, as it turned out, the expression of P450aromatese genes was induced by the transfected mouse SRY DNA.This cell line is currently investigated in order to identify the genes which constitute the putative SRY cascade. 3) Bovine SRY cDNA isolated from a fetal testicular cDNA library is confirmed to possess circular conformation. 4) Caprine c-kit cDNA was cloned and sequenced. It turned out that the intracellular domain of caprine c-kit possess one extra alanine insert which have not been described in any other species except sheep so far examined. The insert appears to be limited to goats and sheep. Attempts are being made … More to determine the biological meaning of the insert. 5) Interesting mutant genes were identified in human patients suffering from the disorders of sexual differetiation. Investigations were made to clarify the function of the gene located on Y chromosome and putatively promote the growth of the body. 6) Detailed analysis of phenotypes of W^f and W^<sh> mouse mutants revealed several interesting features which may be useful to approach the understanding of functioning of c-kit/Sl system in ruminants. 7) Novel genes which might be responsible for the apoptosis of lutcal cells in the rat ovary, were identified. 8) Functions of oncognenes in apoptotic changes in the follicles have been investigated. 9) Role of cyclins in maturation division of oocytes has been investigated. 10) Tissue specificity of the WAP promoter was analyzed, and found to be a 'lcaky' promoter functioning in many tissues other than the mammary gland. 11) Attempts were made to improve efficiency of the production of transgenic animals by selecting embryos which successfully incorporated the transgenes into their genome by combined treatment with restrictions cnzymes and PCR analysis. Less

项目成果

Nakagawa Y,H.Takeuchi et al.: "Restriction fragment length polymorphisms of the CYP11B1 gene in the ........" Jap.J.Human Genet.38. 203-207 (1993)

Nakakawa Y,H.Takeuchi 等人：“……中 CYP11B1 基因的限制性片段长度多态性”Jap.J.Human Genet.38。

Tsutsumi O.,Iida T,et al.: "Intact sex determining region Y (SRY) in a patient with XY pure gonadal dysgenesis and a twin brother." Endocrine Journal. 41. 281-285 (1994)

Tsutsumi O.、Iida T 等人：“XY 纯性腺发育不全患者和双胞胎兄弟的完整性别决定区 Y (SRY)。”

R-Z Lu, et al.: "Activin A and superoxide dismutase syner-gistically enhance development of 1-cell mouse embryos to the blastocyst stage in vitro" J.Reprod.Dev.40. 33-38 (1994)

R-Z Lu 等人：“激活素 A 和超氧化物歧化酶协同促进 1 细胞小鼠胚胎在体外发育至囊胚阶段”J.Reprod.Dev.40。

O.Tsutsumi, et al.: "Intact sex determining region (SRY) in a patient with XY pure gonadal dysgenesis and a twin brother." Endocr.J.41. 281-285 (1994)

O.Tsutsumi 等人：“XY 纯性腺发育不全患者和双胞胎兄弟的完整性别决定区 (SRY)”。

R-Z. Lu,M. Takanashi et al: "Activin A and superoxide dismutase synegistically enhance development of l-cell" Journal of Reproduction and Development. 40. 279-284 (1994)

R-Z。